Summary of Study ST000681
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000481. The data can be accessed directly via it's Project DOI: 10.21228/M8W902 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000681 |
Study Title | Retinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO - TY and LysM cre) |
Study Type | MS analysis |
Study Summary | ABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue. T:RPE specific, L:macrophage specific. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2017-06-05 |
Num Groups | 5 |
Total Subjects | 16 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2017-12-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000481 |
Project DOI: | doi: 10.21228/M8W902 |
Project Title: | Retinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO) |
Project Type: | MS analysis |
Project Summary: | ABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue. |
Institute: | Washington University in St. Louis |
Department: | Department of Ophthalmology and visual sicences |
Laboratory: | Apte Lab |
Last Name: | Ban |
First Name: | Norimitsu |
Address: | 660 S. Euclid Ave. Box 8118 St. Louis, MO 63110 |
Email: | BanN@vision.wustl.edu |
Phone: | 314-362-6690 |
Subject:
Subject ID: | SU000704 |
Subject Type: | Mouse |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | CRE | SEX |
---|---|---|---|
SA039003 | S00027118 | LysM Cre | f |
SA039004 | S00027117 | LysM Cre | f |
SA039005 | S00027116 | LysM Cre | f |
SA039006 | S00027115 | LysM Cre | f |
SA039011 | S00027114 | negative | f |
SA039012 | S00027105 | negative | f |
SA039013 | S00027104 | negative | f |
SA039014 | S00027111 | negative | m |
SA039015 | S00027106 | negative | m |
SA039016 | S00027103 | negative | m |
SA039017 | S00027112 | negative | m |
SA039018 | S00027113 | negative | m |
SA039007 | S00027108 | Tyrosinase Cre | f |
SA039008 | S00027109 | Tyrosinase Cre | m |
SA039009 | S00027107 | Tyrosinase Cre | m |
SA039010 | S00027110 | Tyrosinase Cre | m |
Showing results 1 to 16 of 16 |
Collection:
Collection ID: | CO000698 |
Collection Summary: | - |
Sample Type: | Retina |
Treatment:
Treatment ID: | TR000718 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP000711 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
Analysis ID | AN001048 | AN001049 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | Triple TOF | Triple TOF |
MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE | POSITIVE |
Units | peak area normalized | peak area normalized |
Chromatography:
Chromatography ID: | CH000744 |
Instrument Name: | Shimadzu CTO-20A Nexera X2 |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Column Temperature: | 55 |
Flow Rate: | 0.400uL/min |
Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000943 |
Analysis ID: | AN001048 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
MS ID: | MS000944 |
Analysis ID: | AN001049 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |