Summary of Study ST000684
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000483. The data can be accessed directly via it's Project DOI: 10.21228/M8MS59 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000684 |
| Study Title | Effect of fatty acids on macrophage (wild type versus knockout) lipid levels |
| Study Type | MS analysis |
| Study Summary | Macrophages from wild type or knockout mice with various treatments [none, BSA (control), palmitate + oleate, conditioned media from adipocytes treated with either BSA or palmitate + oleate). N=4/group. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 7 |
| Total Subjects | 40 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000483 |
| Project DOI: | doi: 10.21228/M8MS59 |
| Project Title: | Effect of fatty acids on macrophage lipid levels |
| Project Type: | MS analysis |
| Project Summary: | Effect of fatty acids on macrophage lipid levels |
| Institute: | University of Michigan |
| Department: | Internal Medicine-Nephrology |
| Laboratory: | Pennathur Lab |
| Last Name: | Afshinnia |
| First Name: | Farsad |
| Address: | Ann Arbor, MI |
| Email: | fafshin@med.umich.edu |
| Phone: | 734-615-1293 |
Subject:
| Subject ID: | SU000707 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | genotype | treatment |
|---|---|---|---|
| SA039033 | S00027664 | rage knockout | BSA |
| SA039034 | S00027666 | rage knockout | BSA |
| SA039035 | S00027665 | rage knockout | BSA |
| SA039036 | S00027667 | rage knockout | BSA |
| SA039037 | S00027682 | rage knockout | conditioned media with BSA |
| SA039038 | S00027681 | rage knockout | conditioned media with BSA |
| SA039039 | S00027680 | rage knockout | conditioned media with BSA |
| SA039040 | S00027683 | rage knockout | conditioned media with BSA |
| SA039041 | S00027684 | rage knockout | conditioned media with palm + oleate |
| SA039042 | S00027686 | rage knockout | conditioned media with palm + oleate |
| SA039043 | S00027685 | rage knockout | conditioned media with palm + oleate |
| SA039044 | S00027687 | rage knockout | conditioned media with palm + oleate |
| SA039045 | S00027653 | rage knockout | none |
| SA039046 | S00027654 | rage knockout | none |
| SA039047 | S00027655 | rage knockout | none |
| SA039048 | S00027652 | rage knockout | none |
| SA039049 | S00027671 | rage knockout | palm + oleate |
| SA039050 | S00027669 | rage knockout | palm + oleate |
| SA039051 | S00027670 | rage knockout | palm + oleate |
| SA039052 | S00027668 | rage knockout | palm + oleate |
| SA039053 | S00027658 | wild type | BSA |
| SA039054 | S00027659 | wild type | BSA |
| SA039055 | S00027657 | wild type | BSA |
| SA039056 | S00027656 | wild type | BSA |
| SA039057 | S00027675 | wild type | conditioned media with BSA |
| SA039058 | S00027673 | wild type | conditioned media with BSA |
| SA039059 | S00027674 | wild type | conditioned media with BSA |
| SA039060 | S00027672 | wild type | conditioned media with BSA |
| SA039061 | S00027679 | wild type | conditioned media with palm + oleate |
| SA039062 | S00027678 | wild type | conditioned media with palm + oleate |
| SA039063 | S00027677 | wild type | conditioned media with palm + oleate |
| SA039064 | S00027676 | wild type | conditioned media with palm + oleate |
| SA039065 | S00027650 | wild type | none |
| SA039066 | S00027648 | wild type | none |
| SA039067 | S00027649 | wild type | none |
| SA039068 | S00027651 | wild type | none |
| SA039069 | S00027662 | wild type | palm + oleate |
| SA039070 | S00027661 | wild type | palm + oleate |
| SA039071 | S00027660 | wild type | palm + oleate |
| SA039072 | S00027663 | wild type | palm + oleate |
| Showing results 1 to 40 of 40 |
Collection:
| Collection ID: | CO000701 |
| Collection Summary: | - |
| Sample Type: | Macrophages |
Treatment:
| Treatment ID: | TR000721 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000714 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Chromatography:
| Chromatography ID: | CH000747 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001054 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000747 |
| Num Factors: | 10 |
| Num Metabolites: | 199 |
| Rt Units: | Minutes |
| Units: | peak area normalized |
| Analysis ID: | AN001055 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000747 |
| Num Factors: | 10 |
| Num Metabolites: | 234 |
| Rt Units: | Minutes |
| Units: | peak area normalized |
