Summary of Study ST000687
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000486. The data can be accessed directly via it's Project DOI: 10.21228/M87G8X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000687 |
| Study Title | Nucleotide Analysis following Adenylate Kinase 4 knockdown |
| Study Type | MS analysis |
| Study Summary | Nucleotide Analysis following Adenylate Kinase 4 knockdown |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 2 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000486 |
| Project DOI: | doi: 10.21228/M87G8X |
| Project Title: | Metabolic changes driven by Adenylate Kinase 4 |
| Project Type: | MS analysis |
| Project Summary: | Identify metabolic changed driven by Adenylate Kinase 4 |
| Institute: | Van Andel Research Institute |
| Department: | Center for Cancer and Cell Biology |
| Laboratory: | MacKeigan Lab |
| Last Name: | Lanning |
| First Name: | Nate |
| Address: | 333 Bostwick Ave N.E. Grand Rapids, MI 49503 |
| Email: | nate.lanning@vai.org |
| Phone: | 000-000-0000 |
Subject:
| Subject ID: | SU000710 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | TYPE |
|---|---|---|
| SA039118 | S00012747 | AK4 |
| SA039119 | S00012748 | AK4 |
| SA039120 | S00012749 | AK4 |
| SA039121 | S00012746 | AK4 |
| SA039122 | S00012745 | AK4 |
| SA039123 | S00012741 | Control |
| SA039124 | S00012742 | Control |
| SA039125 | S00012743 | Control |
| SA039126 | S00012744 | Control |
| SA039127 | S00012740 | Control |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000704 |
| Collection Summary: | - |
Treatment:
| Treatment ID: | TR000724 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000717 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A012_-_NAD-Related_Metabolites.pdf |
Chromatography:
| Chromatography ID: | CH000750 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Gradient: | 0min, 80%B, 15min, 0%B, 20min, 0%B, 35min, 80%B |
| Flow Rate: | 75ul/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN001060 |
| Analysis Type: | MS |
| Analysis Protocol File: | A012_-_NAD-Related_Metabolites.pdf |
| Instrument Name: | 6520B Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000750 |
| Num Factors: | 2 |
| Num Metabolites: | 17 |
| Units: | nmol/plate |
