Summary of Study ST000687

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000486. The data can be accessed directly via it's Project DOI: 10.21228/M87G8X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000687
Study TitleNucleotide Analysis following Adenylate Kinase 4 knockdown
Study TypeMS analysis
Study SummaryNucleotide Analysis following Adenylate Kinase 4 knockdown
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M87G8X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000486
Project DOI:doi: 10.21228/M87G8X
Project Title:Metabolic changes driven by Adenylate Kinase 4
Project Type:MS analysis
Project Summary:Identify metabolic changed driven by Adenylate Kinase 4
Institute:Van Andel Research Institute
Department:Center for Cancer and Cell Biology
Laboratory:MacKeigan Lab
Last Name:Lanning
First Name:Nate
Address:333 Bostwick Ave N.E. Grand Rapids, MI 49503
Email:nate.lanning@vai.org
Phone:000-000-0000

Subject:

Subject ID:SU000710
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id TYPE
SA039118S00012747AK4
SA039119S00012748AK4
SA039120S00012749AK4
SA039121S00012746AK4
SA039122S00012745AK4
SA039123S00012741Control
SA039124S00012742Control
SA039125S00012743Control
SA039126S00012744Control
SA039127S00012740Control
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000704
Collection Summary:-

Treatment:

Treatment ID:TR000724
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000717
Sampleprep Summary:-
Sampleprep Protocol Filename:A012_-_NAD-Related_Metabolites.pdf

Combined analysis:

Analysis ID AN001060
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520B QTOF
Ion Mode NEGATIVE
Units nmol/plate

Chromatography:

Chromatography ID:CH000750
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Flow Gradient:0min, 80%B, 15min, 0%B, 20min, 0%B, 35min, 80%B
Flow Rate:75ul/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS000955
Analysis ID:AN001060
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A012_-_NAD-Related_Metabolites.pdf
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