Summary of study ST000690

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000489. The data can be accessed directly via it's Project DOI: 10.21228/M8V89T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000690
Study TitleHuman oral keratinocytes growth and proliferation based on culture medium volume
Study TypeMS analysis
Study SummaryWe seeded cells at density of 500k/T75 and cultured them at two different medium volume, one at 30ml, the other at 15ml. Culture medium is composed of 1x EDGS and 0.06mM calcium. We changed medium every day for 30ml culture, and every two days for 15ml culture. We collected spent medium when we changed medium. The collected spent medium was filtered through low protein binding 0.22um pore size filter and then stored at -80 degree C. For OA and SMT, spent medium was collected on the same day of D5 and D9 after cell seeding as marked on the tubes. For GK samples, 15ml and 30ml cultures were collected when cells reached at around 90% confluence, that is D5 for 15ml and D6 for 30 ml culture. The numbers 151, 152 were duplicated cell cultures in two T75s, same for 301 and 302. One major differences among GK, OA, and SMT is GK was from frozen cells; OA and SMT were fresh cells that were never frozen. Blank15 and Blank30 were complete medium without cells incubating in incubator for two days for Blank15 and one day for Blank30. Medium was collected in the same manner as others.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups10
Total Subjects5
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8V89T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000489
Project DOI:doi: 10.21228/M8V89T
Project Title:Human oral keratinocytes growth and proliferation based on culture medium volume
Project Type:MS analysis
Project Summary:Using different culture medium volume to affect cultured human oral keratinocytes growth and proliferation rates
Institute:University of Michigan
Department:Oral & Maxillofacial Surgery
Laboratory:Feinberg Lab
Last Name:Feinberg
First Name:Stephen
Address:Ann Arbor, MI
Email:sefein@umich.edu
Phone:734-763-5963

Subject:

Subject ID:SU000713
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Day Line Volume
SA039169S000143192 days Blank 15ml
SA039170S000143202 days Blank 30ml
SA039171S000143085 days GK 15ml
SA039172S000143075 days GK 15ml
SA039173S000143115 days OA 15ml
SA039174S000143135 days OA 30ml
SA039175S000143155 days SMT 15ml
SA039176S000143175 days SMT 30ml
SA039177S000143096 days GK 30ml
SA039178S000143106 days GK 30ml
SA039179S000143129 days OA 15ml
SA039180S000143149 days OA 30ml
SA039181S000143169 days SMT 15ml
SA039182S000143189 days SMT 30ml
SA039168S00020128- - -
Showing results 1 to 15 of 15

Collection:

Collection ID:CO000707
Collection Summary:-
Sample Type:Keratinocytes

Treatment:

Treatment ID:TR000727
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000720
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001065 AN001066
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000753
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000960
Analysis ID:AN001065
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000961
Analysis ID:AN001066
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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