Summary of study ST000702

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000499. The data can be accessed directly via it's Project DOI: 10.21228/M8JS5P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000702
Study TitleNIH 3T3 fibroblast cells with Ceftriaxone Treatment
Study TypeMS analysis
Study SummaryNIH 3T3 cells were plated at 1.5million cells per 10cM dish into 12 10cm dishes. 24hrs post plating media was changed on all plates. At the time of media change, 4 dishes were treated with a final concentration of 50uM ceftriaxone (24hrs ceftriaxone n=4). 23 hrs post media change, 4 separate dishes were treated with 50uM ceftriaxone for 1hr (1hr ceftriaxone n=4). 4 dishes were untreated (0hr, basal, n=4). 24 hrs post media change, media was quickly removed from all 12 plates, each plate was rapidly washed with MilliQ H2O and rapidly frozen with liquid nitrogen poured directly into the dishes. All plates were transferred to dry ice and stored at -80 until shipped.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups3
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8JS5P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000499
Project DOI:doi: 10.21228/M8JS5P
Project Title:NIH 3T3 fibroblast cells with Ceftriaxone Treatment
Project Type:MS analysis
Project Summary:3T3 cells treated with 50uM Ceftriaxone for 24hrs, 1hr, or 0 hrs.
Institute:Vanderbilt University
Department:Pharmacology
Laboratory:Blakely Lab
Last Name:Blakely
First Name:Randy
Address:Nashville, TN
Email:randy.blakely@vanderbilt.edu
Phone:615-936-1700

Subject:

Subject ID:SU000725
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10088
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Timepoint
SA039539S00020154-
SA039540S000193310hr
SA039541S000193340hr
SA039542S000193320hr
SA039543S000193330hr
SA039544S000193381hr
SA039545S000193371hr
SA039546S000193361hr
SA039547S000193351hr
SA039548S0001934224hr
SA039549S0001934124hr
SA039550S0001934024hr
SA039551S0001933924hr
Showing results 1 to 13 of 13

Collection:

Collection ID:CO000719
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000739
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000732
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001091 AN001092
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000767
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000986
Analysis ID:AN001091
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000987
Analysis ID:AN001092
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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