Summary of Study ST000724

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000521. The data can be accessed directly via it's Project DOI: 10.21228/M89X00 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000724
Study TitleRed squirrels age related changes
Study TypeMS analysis
Study SummaryMetabolomics has recently been used to document age-related changes in key metabolic pathways in laboratory animals and as a biomarker to predict age. We study the ecology, evolution, behavior, and physiology of wild North American red squirrels where we are able to follow individual squirrels across their lifetime from birth until death. We are beginning to document aging in this natural population and are interested in understanding whether there is a signature of aging using metabolomics. We collected plasma samples from the oldest female and male squirrels in our study population and also from an equivalent number of younger squirrels. We will use an untargeted approach to provide an assessment of what metabolites differ among very old and young squirrels. The aim of these analyses is to allow us to identify if the same metabolites that have been identified as biomarkers of advanced age from laboratory mice are also biomarkers of advanced age in red squirrels. After we complete these untargeted analyses, we aim to develop a metabolomics panel for this species so that we can use a more targeted approach to assess how the metabolic profiles of specific pathways (glucose/fatty acid metabolism, redox homeostasis) change with age in offspring exposed to increased perinatal stress in our study species.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups6
Total Subjects33
Study CommentsMetabolomics has recently been used to document age-related changes in key metabolic pathways in laboratory animals and as a biomarker to predict age. We study the ecology, evolution, behavior, and physiology of wild North American red squirrels where we are able to follow individual squirrels across their lifetime from birth until death. We are beginning to document aging in this natural population and are interested in understanding whether there is a signature of aging using metabolomics.
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M89X00
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000521
Project DOI:doi: 10.21228/M89X00
Project Title:Age related changes in metabolomics in red squirrels
Project Type:MS analysis
Project Summary:Pilot analyses for age-related changes in physiology in wild North American red squirrels
Institute:University of Michigan
Department:Psychology
Laboratory:Dantzer Lab
Last Name:Dantzer
First Name:Ben
Address:Ann Arbor, MI
Email:dantzer@umich.edu
Phone:734-615-2352

Subject:

Subject ID:SU000747
Subject Type:Animal
Subject Species:Tamiasciurus hudsonicus
Taxonomy ID:10009
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Tamiasciurus hudsonicus (Factor headings shown in green)

mb_sample_id local_sample_id Age Category Sex of individual
SA040688S00026266Old Female
SA040689S00026261Old Female
SA040690S00026258Old Female
SA040691S00026260Old Female
SA040692S00026264Old Female
SA040693S00026242Old Female
SA040694S00026263Old Female
SA040695S00026262Old Male
SA040696S00026257Old Male
SA040697S00026234Old Male
SA040698S00026265Old Male
SA040699S00026259Old Male
SA040700S00026255Old Male
SA040701S00026239Old Male
SA040702S00026241Old Male
SA040703S00026236Old Male
SA040704S00026249Old Young
SA040705S00026253Unknown Male
SA040706S00026256Young Female
SA040707S00026247Young Female
SA040708S00026238Young Female
SA040709S00026237Young Female
SA040710S00026245Young Female
SA040711S00026251Young Female
SA040712S00026252Young Female
SA040713S00026254Young Female
SA040714S00026250Young Male
SA040715S00026240Young Male
SA040716S00026235Young Male
SA040717S00026243Young Male
SA040718S00026244Young Male
SA040719S00026246Young Male
SA040720S00026248Young Male
Showing results 1 to 33 of 33

Collection:

Collection ID:CO000741
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000761
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000754
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001134 AN001135
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000797
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001029
Analysis ID:AN001134
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001030
Analysis ID:AN001135
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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