Summary of Study ST000736
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000533. The data can be accessed directly via it's Project DOI: 10.21228/M8S10C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000736 |
| Study Title | Metabolic change in macrophages after efferocytosis |
| Study Type | MS analysis |
| Study Summary | Study metabolic pathway alteration in macrophages after efferocytosis (when macrophages eating apoptotic cells). Peritoneal macrophages were purified and plated in the cell-culture dishes. After culturing overnight. Apoptotic cells were overlayed to macrophages for 2 hours, then un-engulfed cells were removed through washing. Macrophages were left in the plate for another 4 hours before being lifted from the plate. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 5 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000533 |
| Project DOI: | doi: 10.21228/M8S10C |
| Project Title: | Metabolic change in macrophages after efferocytosis |
| Project Type: | MS analysis |
| Project Summary: | To study metabolic change in macrophages after efferocytosis |
| Institute: | Northwestern University |
| Department: | Pathology |
| Laboratory: | Thorp Lab |
| Last Name: | Thorp |
| First Name: | Edward |
| Address: | Evanston, IL |
| Email: | ebthorp@northwestern.edu |
| Phone: | 312-503-4309 |
Subject:
| Subject ID: | SU000759 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA041101 | S00020917 | apoptotic cells for 2 hours |
| SA041102 | S00020916 | apoptotic cells for 2 hours |
| SA041103 | S00020915 | apoptotic cells for 2 hours |
| SA041104 | S00020921 | ko treated with apoptotic cells 2 hrs |
| SA041105 | S00020922 | ko treated with apoptotic cells 2 hrs |
| SA041106 | S00020920 | ko treated with apoptotic cells 2hrs |
| SA041107 | S00020918 | ko untreated |
| SA041108 | S00020919 | ko untreated |
| SA041109 | S00020914 | none |
| SA041110 | S00020913 | none |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000753 |
| Collection Summary: | - |
| Sample Type: | Macrophages |
Treatment:
| Treatment ID: | TR000773 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000766 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A011_-_Glycolysis-TCA-nucleotides.pdf |
Chromatography:
| Chromatography ID: | CH000810 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN001150 |
| Analysis Type: | MS |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
| Instrument Name: | 6520B Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000810 |
| Num Factors: | 5 |
| Num Metabolites: | 15 |
| Units: | counts |
