Summary of study ST000737

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000533. The data can be accessed directly via it's Project DOI: 10.21228/M8S10C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000737
Study TitleMetabolic change in macrophages after efferocytosis (part II)
Study TypeMS analysis
Study SummaryThe goal of the experiment is to study metabolic pathway alteration in macrophages after efferocytosis (when macrophages eating apoptotic cells). Peritoneal macrophages were purified and plated in the cell-culture dishes. After culturing overnight. Apoptotic cells were overlayed to macrophages for 2 hours, then un-engulfed cells were removed through washing. Macrophages were left in the plate for another 4 hours before being lifted from the plate.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8S10C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000533
Project DOI:doi: 10.21228/M8S10C
Project Title:Metabolic change in macrophages after efferocytosis
Project Type:MS analysis
Project Summary:To study metabolic change in macrophages after efferocytosis
Institute:Northwestern University
Department:Pathology
Laboratory:Thorp Lab
Last Name:Thorp
First Name:Edward
Address:Evanston, IL
Email:ebthorp@northwestern.edu
Phone:312-503-4309

Subject:

Subject ID:SU000760
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA041111S00024017apoptotic cells for 2hrs
SA041112S00024016apoptotic cells for 2hrs
SA041113S00024015apoptotic cells for 2hrs
SA041114S00024021ko treated with apoptotic cells 2hrs
SA041115S00024022ko treated with apoptotic cells 2hrs
SA041116S00024020ko treated with apoptotic cells 2hrs
SA041117S00024018ko untreated
SA041118S00024019ko untreated
SA041119S00024014none
SA041120S00024013none
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000754
Collection Summary:-
Sample Type:Macrophages

Treatment:

Treatment ID:TR000774
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000767
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_-_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001151
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Agilent HP5-MS (15m × 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units pmol/ug protein

Chromatography:

Chromatography ID:CH000811
Instrument Name:Agilent 7890N
Column Name:Agilent HP5-MS (15m × 0.25mm, 0.25 um)
Chromatography Type:GC

MS:

MS ID:MS001046
Analysis ID:AN001151
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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