Summary of study ST000753

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000543. The data can be accessed directly via it's Project DOI: 10.21228/M8GH3J This work is supported by NIH grant, U2C- DK119886.

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Study IDST000753
Study TitleComparison of the metabolome and lipidome of wild type and mdx/mTR mice
Study TypeMS analysis
Study SummaryThere are two groups of mice in the study, wild type mice (WT) and mdx/mTR mice (mdx or mdx/mTR) that are a model of musclar dystrophy. We collected the tibialis anterior muscles from these mice (N=6 in each group), and would like to perform TCA-plus analysis and shotgun lipidomics analysis on the mice, and compare across the two groups.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8GH3J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000543
Project DOI:doi: 10.21228/M8GH3J
Project Title:Comparison of the metabolome and lipidome of wild type and mdx/mTR mice
Project Type:MS analysis
Project Summary:A comparison of the metabolome and lipidome of wild type and mdx/mTR mice
Institute:University of Michigan
Department:Orthopaedic Surgery
Laboratory:Mendias Lab
Last Name:Mendias
First Name:Christopher
Address:Ann Arbor, MI
Email:cmendias@umich.edu
Phone:734-764-3250

Subject:

Subject ID:SU000776
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA041490S00025567mdx/mTR
SA041491S00025566mdx/mTR
SA041492S00025562mdx/mTR
SA041493S00025563mdx/mTR
SA041494S00025564mdx/mTR
SA041495S00025565mdx/mTR
SA041484S00025556WT
SA041485S00025561WT
SA041486S00025560WT
SA041487S00025557WT
SA041488S00025558WT
SA041489S00025559WT
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000770
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000790
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000783
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001181 AN001182 AN001183
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase HILIC
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2 Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI ESI ESI
MS instrument type Triple TOF Triple TOF QTOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF Agilent 6520B QTOF
Ion Mode NEGATIVE POSITIVE NEGATIVE
Units peak area normalized peak area normalized counts

Chromatography:

Chromatography ID:CH000829
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000830
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001076
Analysis ID:AN001181
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001077
Analysis ID:AN001182
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001078
Analysis ID:AN001183
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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