Summary of study ST000757

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000546. The data can be accessed directly via it's Project DOI: 10.21228/M8397K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000757
Study TitleTubuloglomerular feedback and salt-sensitive hypertension
Study TypeMS analysis
Study SummaryCollect whole blood in tubes containing EDTA, gently invert tubes 10 times and cool as soon as practical on ice. Centrifuge to separate plasma as soon as practical, transfer to appropriately labeled tubes, and freeze immediately. I test basal glucose and plasma store plasma in -20C for one day then transfer to -80C. The next day, inject STZ 60 mg/kg ip. After 3 days, check for increased glucose and extract plasma for early stage diabetic sample >300 mg/dl. Store in -20C and then -80C. 30 days after initial injection, extract plasma and store in -20C and then -80C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups9
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8397K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000546
Project DOI:doi: 10.21228/M8397K
Project Title:Tubuloglomerular feedback and salt-sensitive hypertension
Project Type:MS analysis
Project Summary:When TGF changes, does metabolics change
Institute:University of South Florida
Department:Molecular Pharmacology & Physiology
Laboratory:Liu Lab
Last Name:Liu
First Name:Ruisheng
Address:Tampa, FL
Email:ruisheng@health.usf.edu
Phone:813-396-9107

Subject:

Subject ID:SU000780
Subject Type:Rat
Subject Species:Rattus
Taxonomy ID:10114
Species Group:Mammal

Factors:

Subject type: Rat; Subject species: Rattus (Factor headings shown in green)

mb_sample_id local_sample_id Days Gene STZ injection
SA041605S0001935130 days non wild type yes
SA041606S0001934830 days non wild type yes
SA041607S0001934530 days non wild type yes
SA041602S000193443 days non wild type yes
SA041603S000193503 days non wild type yes
SA041604S000193463 days wild type no
SA041608S00019347NA non wild type yes
SA041609S00019349NA wild type no
SA041610S00019343NA wild type no
SA041611S00020155Pooled Pooled plasma Pooled plasma
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000774
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000794
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000787
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001188 AN001189
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000834
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001083
Analysis ID:AN001188
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001084
Analysis ID:AN001189
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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