Summary of study ST000767

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000553. The data can be accessed directly via it's Project DOI: 10.21228/M86095 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000767
Study TitleMetabolite analysis of regional neural stem/progenitor cells
Study TypeMS analysis
Study SummaryThe developing mammalian brain generates a variety of Neural Progenitor Cells (NPCs). Primary NPCs throughout the neuraxis are derived from the ventricular zone. Intermediate progenitor cells (IPCs) are produced uniquely in the telencephalon and contribute extensively to the neurons that comprise the cerebral cortex and basal ganglia. It is known that the fate of the diverse NPC populations is determined by the interplay of transcription factors and regulation by regional humoral cues. However, despite our recent appreciation that nutrient-regulated intracellular metabolic milieu (pO2, energy, and redox state) significantly influence cell fate, an unexplored area is whether NPCs have intrinsic metabolic identity, and if so, the mechanism by which molecular metabolism contributes to brain development.Little is known however, if intrinsic differences in cellular metabolism of regional NPCs make certain NPCs susceptible while others resistant to genetic and environmental insults. We conjectured that regional (fore-and hindbrain) NPCs are metabolically distinct.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s).D
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M86095
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000553
Project DOI:doi: 10.21228/M86095
Project Title:Metabolic uniqueness of neural stem/progenitor cells Studies
Project Type:MS analysis
Project Summary:Cell-intrinsic metabolic uniqueness of regional neural stem/progenitor cells
Institute:Cincinnati Children's Hospital Medical Center
Department:Oncology
Laboratory:Dasgupta Lab
Last Name:Dasgupta
First Name:Biplab
Address:Cincinnati, OH
Email:biplab.dasgupta@cchmc.org
Phone:513-803-1370

Subject:

Subject ID:SU000790
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id NPC source
SA042081S00026132forebrain
SA042082S00026133forebrain
SA042083S00026134hindbrain
SA042084S00026135hindbrain
Showing results 1 to 4 of 4

Collection:

Collection ID:CO000784
Collection Summary:-
Sample Type:Stem cells

Treatment:

Treatment ID:TR000804
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000797
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_-_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001213
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent
Column Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520B QTOF
Ion Mode NEGATIVE
Units pmol/mg protein

Chromatography:

Chromatography ID:CH000850
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001108
Analysis ID:AN001213
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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