Summary of Study ST000769

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000555. The data can be accessed directly via it's Project DOI: 10.21228/M8XM3M This work is supported by NIH grant, U2C- DK119886.


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Study IDST000769
Study TitleMetabolomics of Lin-Sca1+Kit+ (LSK) cells in NM mice
Study TypeMS analysis
Study SummaryLSKs from transgenic mice are harvested and sorted via flow cytometry. They are collected after two sorts as DAPI-, Lineage-, Sca1+, c-kit+ cells.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000555
Project DOI:doi: 10.21228/M8XM3M
Project Title:Oncogenic Ras-induced metabolic perturbations
Project Type:MS analysis
Project Summary:Oncogenic Ras-induced metabolic perturbations
Institute:University of Michigan
Department:Internal Med and Cellular and Molecular Biology
Laboratory:Li, Qing Lab
Last Name:Li
First Name:Qing
Address:Ann Arbor, MI


Subject ID:SU000792
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal


Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Type
SA042090S00026009mutant ras
SA042089S00026008WT ras
Showing results 1 to 2 of 2


Collection ID:CO000786
Collection Summary:-
Sample Type:Bone marrow


Treatment ID:TR000806
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000799
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_-_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001216
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Agilent HP5-MS (15m × 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Units uM/ng protein


Chromatography ID:CH000852
Instrument Name:Agilent 7890N
Column Name:Agilent HP5-MS (15m × 0.25mm, 0.25 um)
Chromatography Type:GC


MS ID:MS001111
Analysis ID:AN001216
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Analysis Protocol File:A011_-_Glycolysis-TCA-nucleotides.pdf