Summary of Study ST000774

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000560. The data can be accessed directly via it's Project DOI: 10.21228/M88T0N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000774
Study TitleMetabolic effects of novel aldehyde dehydrogenase inhibitors (ALDH) inhibitors
Study TypeMS analysis
Study SummaryEffect of novel aldehyde dehydrogenase inhibitors. Before an experiment medium was replaced to medium without glucose with addition of labeled glucose on C13. Next, cells were treated with ALDH inhibitors 673 or 773 for 1, 4, or 8 hr (673&773-Jan26)
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups9
Total Subjects36
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M88T0N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000560
Project DOI:doi: 10.21228/M88T0N
Project Title:Metabolic effects of novel aldehyde dehydrogenase inhibitors (ALDH) inhibitors
Project Type:MS analysis
Project Summary:Effect of novel aldehyde dehydrogenase inhibitors (N3 Flux-673-773)
Institute:University of Michigan
Department:Hemtology/Oncology
Laboratory:Buckanovich Lab
Last Name:Buckanovich
First Name:Ronald
Address:Ann Arbor, MI
Email:ronaldbu@umich.edu
Phone:734-764-2395

Subject:

Subject ID:SU000797
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id cell type time treatment
SA042282S00017249Cd133 1hr Compound 673
SA042283S00017251Cd133 1hr Compound 673
SA042284S00017250Cd133 1hr Compound 673
SA042285S00017253Cd133 1hr Compound 773
SA042286S00017252Cd133 1hr Compound 773
SA042287S00017254Cd133 1hr Compound 773
SA042288S00017257Cd133 4hr Compound 673
SA042289S00017256Cd133 4hr Compound 673
SA042290S00017255Cd133 4hr Compound 673
SA042291S00017260Cd133 4hr Compound 773
SA042292S00017259Cd133 4hr Compound 773
SA042293S00017258Cd133 4hr Compound 773
SA042294S00017263Cd133 8hr Compound 673
SA042295S00017262Cd133 8hr Compound 673
SA042296S00017261Cd133 8hr Compound 673
SA042297S00017265Cd133 8hr Compound 773
SA042298S00017266Cd133 8hr Compound 773
SA042299S00017264Cd133 8hr Compound 773
SA042300S00017246Cd133 NA Control
SA042301S00017247Cd133 NA Control
SA042302S00017248Cd133 NA Control
SA042303S00017270OvCAR8 4hr Compound 673
SA042304S00017272OvCAR8 4hr Compound 673
SA042305S00017271OvCAR8 4hr Compound 673
SA042306S00017275OvCAR8 4hr Compound 773
SA042307S00017274OvCAR8 4hr Compound 773
SA042308S00017273OvCAR8 4hr Compound 773
SA042309S00017277OvCAR8 8hr Compound 673
SA042310S00017276OvCAR8 8hr Compound 673
SA042311S00017278OvCAR8 8hr Compound 673
SA042312S00017281OvCAR8 8hr Compound 773
SA042313S00017280OvCAR8 8hr Compound 773
SA042314S00017279OvCAR8 8hr Compound 773
SA042315S00017267OvCAR8 NA Control
SA042316S00017269OvCAR8 NA Control
SA042317S00017268OvCAR8 NA Control
Showing results 1 to 36 of 36

Collection:

Collection ID:CO000791
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000811
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000804
Sampleprep Summary:-
Sampleprep Protocol Filename:A016 - Vitamins.pdf

Combined analysis:

Analysis ID AN001221
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent
Column C18 (150 x 2mm) (Waters Corporation)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode POSITIVE
Units pmol/ug protein

Chromatography:

Chromatography ID:CH000857
Instrument Name:Agilent
Column Name:C18 (150 x 2mm) (Waters Corporation)
Chromatography Type:Reversed phase

MS:

MS ID:MS001116
Analysis ID:AN001221
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A016 - Vitamins.pdf
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