Summary of study ST000838

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000598. The data can be accessed directly via it's Project DOI: 10.21228/M8CD6Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000838
Study TitleObesity and marrow and marrow adipose tissue (MAT) metabolomics
Study TypeMS analysis
Study SummaryMale C57Bl6j mice were fed HFD for 16-20 weeks and marrow adipose tissue (MAT) and bone marrow collected to evaluate lipid content/lipid type. In addition, marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be performed.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups4
Total Subjects6
Raw Data AvailableYes
Raw Data File Type(s).D
Analysis Type DetailLC-MS
Release Date2018-08-27
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8CD6Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000598
Project DOI:doi: 10.21228/M8CD6Q
Project Title:Obesity and marrow and MAT metabolomics Studies
Project Type:MS analysis
Project Summary:MAT and bone marrow collected to evaluate lipid content/lipid type. In addition, marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be performed.
Institute:University of Michigan
Department:Pedatric Endocrinology
Laboratory:Singer Lab
Last Name:Singer
First Name:Kanakadurga
Address:Ann Arbor, MI
Email:ksinger@umich.edu
Phone:734-764-5175

Subject:

Subject ID:SU000865
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Sample type
SA046636S00028172HFD Adipose Tissue
SA046637S00028170HFD Adipose Tissue
SA046638S00028171HFD Adipose Tissue
SA046639S00028177HFD Bone Marrow
SA046640S00028176HFD Bone Marrow
SA046641S00028178HFD Bone Marrow
SA046642S00028167ND Adipose Tissue
SA046643S00028169ND Adipose Tissue
SA046644S00028168ND Adipose Tissue
SA046645S00028173ND Bone Marrow
SA046646S00028174ND Bone Marrow
SA046647S00028175ND Bone Marrow
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000859
Collection Summary:-
Sample Type:Bone marrow

Treatment:

Treatment ID:TR000879
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000872
Sampleprep Summary:-
Sampleprep Protocol Filename:A008_Acyl_Carnitines.pdf

Combined analysis:

Analysis ID AN001342 AN001343 AN001344
Analysis type MS MS MS
Chromatography type Reversed phase HILIC HILIC
Chromatography system Agilent Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Phenomenex Luna NH2 (150 x 1mm, 3um) Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI ESI ESI
MS instrument type Triple quadrupole QTOF QTOF
MS instrument name Agilent 6490A QQQ Agilent 6520B QTOF Agilent 6520B QTOF
Ion Mode POSITIVE NEGATIVE NEGATIVE
Units uM pmol/mg counts/mg

Chromatography:

Chromatography ID:CH000938
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000939
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH000940
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001234
Analysis ID:AN001342
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001235
Analysis ID:AN001343
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001236
Analysis ID:AN001344
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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