Summary of Study ST000841

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000599. The data can be accessed directly via it's Project DOI: 10.21228/M87Q3F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000841
Study TitleMetabolomics of muscle in insulin sensitive and resistant obese indivduals.
Study TypeMS analysis
Study SummaryOC samples: muscle biopsy samples collected from insulin sensitive / insulin resistant obese indivduals before undergoing a hyperinsulinemic-euglycemic clamp. Subjects were previously categorized for the rate of fatty acid release from adipose tissue into the bloodstream as a measure of insuline resistance. Low-FA, Med-FA, and High-FA stand for low, medium, and high rate of release.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups4
Total Subjects28
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M87Q3F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000599
Project DOI:doi: 10.21228/M87Q3F
Project Title:Prelim data for NIH exercise consortium
Project Type:MS analysis
Project Summary:Plasma samples before and after exercise training in obese adults
Institute:University of Michigan
Department:Kinesiology
Laboratory:Horowitz Lab
Last Name:Horowitz
First Name:Jeff
Address:Ann Arbor, MI
Email:jeffhoro@umich.edu
Phone:734-647-3354

Subject:

Subject ID:SU000868
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Subject group
SA046693S00020440HIGH-FA
SA046694S00020450HIGH-FA
SA046695S00020451HIGH-FA
SA046696S00020449HIGH-FA
SA046697S00020452HIGH-FA
SA046698S00020447HIGH-FA
SA046699S00020445HIGH-FA
SA046700S00020448HIGH-FA
SA046701S00020439HIGH-FA
SA046702S00020427LOW-FA
SA046703S00020442LOW-FA
SA046704S00020436LOW-FA
SA046705S00020430LOW-FA
SA046706S00020428LOW-FA
SA046707S00020431LOW-FA
SA046708S00020433LOW-FA
SA046709S00020434LOW-FA
SA046710S00020446MID-FA
SA046711S00020441MID-FA
SA046712S00020432MID-FA
SA046713S00020429MID-FA
SA046714S00020444MID-FA
SA046715S00020435MID-FA
SA046716S00020437MID-FA
SA046717S00020438MID-FA
SA046718S00020443MID-FA
SA046719S00020453N/A
SA046720S00020454N/A
Showing results 1 to 28 of 28

Collection:

Collection ID:CO000862
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000882
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000875
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Chromatography:

Chromatography ID:CH000944
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000945
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH000946
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Rate:0.250 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase
  
Chromatography ID:CH000947
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Flow Rate:0.075 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC

Analysis:

Analysis ID:AN001349
Analysis Type:MS
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
Instrument Name:6530 Q-TOF LC/MS (Agilent)
Chromatography ID:CH000944
Num Factors:4
Num Metabolites:81
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST000841_AN001349_Results.txt
Units:counts
  
Analysis ID:AN001350
Analysis Type:MS
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
Instrument Name:6530 Q-TOF LC/MS (Agilent)
Chromatography ID:CH000944
Num Factors:4
Num Metabolites:77
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST000841_AN001350_Results.txt
Units:Counts
  
Analysis ID:AN001351
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000945
Num Factors:4
Num Metabolites:156
Rt Units:Minutes
Units:counts norm
  
Analysis ID:AN001352
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000945
Num Factors:4
Num Metabolites:231
Rt Units:Minutes
Units:counts norm
  
Analysis ID:AN001353
Analysis Type:MS
Analysis Protocol File:A008_-_Acyl_Carnitines.pdf
Instrument Name:6490A QQQ LC/MS (Agilent)
Chromatography ID:CH000946
Num Factors:4
Num Metabolites:27
Units:pmol/mg
  
Analysis ID:AN001354
Analysis Type:MS
Analysis Protocol File:A011_-_Glycolysis-TCA-nucleotides.pdf
Instrument Name:6520B Q-TOF LC/MS (Agilent)
Chromatography ID:CH000947
Num Factors:4
Num Metabolites:7
Units:counts
  
Analysis ID:AN001355
Analysis Type:MS
Analysis Protocol File:A011_-_Glycolysis-TCA-nucleotides.pdf
Instrument Name:6520B Q-TOF LC/MS (Agilent)
Chromatography ID:CH000947
Num Factors:4
Num Metabolites:23
Units:ug/100 mg
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