Summary of Study ST000883
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000612. The data can be accessed directly via it's Project DOI: 10.21228/M8FM7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000883 |
| Study Title | Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants |
| Study Summary | Current evidence suggests that malaria infection could alter patient breath metabolites, a phenomenon that could be exploited to create a breath-based diagnostic test. Indications include the preferential attraction of the Anopheles mosquito vector upon infection and a distinct breath profile with the progression of experimental, sub-microscopic malaria. However, these observations have yet to be extended to the clinic. To investigate whether natural human malaria infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from pediatric patients with and without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we find that children with malaria have a distinct shift in overall breath composition. Leveraging these differences, highly accurate classification of infection status was achieved with a suite of six compounds. In addition, we find that malaria-infected children have significantly higher breath levels of two mosquito-attractant terpenes, α-pinene and 3-carene. Thus, our work attests to the viability of breath analysis for malaria diagnosis, identifies candidate compounds for follow-up studies, and identifies biologically plausible chemical mediators for increased mosquito attraction to malaria-infected patients. |
| Institute | Washington University in St. Louis |
| Department | School of Medicine |
| Last Name | Schaber |
| First Name | Chad |
| Address | 4938 Parkview Place, MPRB/FLoor 6, Entry 5, St. Louis, MO, 63110, USA |
| chadschaber@wustl.edu | |
| Phone | 3142862040 |
| Submit Date | 2017-10-08 |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-02-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000612 |
| Project DOI: | doi: 10.21228/M8FM7S |
| Project Title: | Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants |
| Project Summary: | Current evidence suggests that malaria infection could alter patient breath metabolites, a phenomenon that could be exploited to create a breath-based diagnostic test. Indications include the preferential attraction of the Anopheles mosquito vector upon infection and a distinct breath profile with the progression of experimental, sub-microscopic malaria. However, these observations have yet to be extended to the clinic. To investigate whether natural human malaria infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from pediatric patients with and without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we find that children with malaria have a distinct shift in overall breath composition. Leveraging these differences, highly accurate classification of infection status was achieved with a suite of six compounds. In addition, we find that malaria-infected children have significantly higher breath levels of two mosquito-attractant terpenes, α-pinene and 3-carene. Thus, our work attests to the viability of breath analysis for malaria diagnosis, identifies candidate compounds for follow-up studies, and identifies biologically plausible chemical mediators for increased mosquito attraction to malaria-infected patients. |
| Institute: | Washington University in St. Louis |
| Department: | School of Medicine |
| Last Name: | Schaber |
| First Name: | Chad |
| Address: | 4938 Parkview Place, MPRB/FLoor 6, Entry 5, St. Louis, MO, 63110, USA |
| Email: | chadschaber@wustl.edu |
| Phone: | 3142862040 |
Subject:
| Subject ID: | SU000917 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Malaria Infection Status |
|---|---|---|
| SA051168 | Patient 14 | Negative |
| SA051169 | Patient 13 | Negative |
| SA051170 | Patient 12 | Negative |
| SA051171 | Patient 15 | Negative |
| SA051172 | Patient 16 | Negative |
| SA051173 | Patient 1 | Negative |
| SA051174 | Patient 17 | Negative |
| SA051175 | Patient 11 | Negative |
| SA051176 | Patient 18 | Negative |
| SA051177 | Patient 4 | Negative |
| SA051178 | Patient 3 | Negative |
| SA051179 | Patient 10 | Negative |
| SA051180 | Patient 5 | Negative |
| SA051181 | Patient 2 | Negative |
| SA051182 | Patient 9 | Negative |
| SA051183 | Patient 6 | Negative |
| SA051184 | Patient 8 | Negative |
| SA051185 | Patient 7 | Negative |
| SA051186 | Patient 31 | Positive |
| SA051187 | Patient 29 | Positive |
| SA051188 | Patient 32 | Positive |
| SA051189 | Patient 30 | Positive |
| SA051190 | Patient 34 | Positive |
| SA051191 | Patient 28 | Positive |
| SA051192 | Patient 35 | Positive |
| SA051193 | Patient 33 | Positive |
| SA051194 | Patient 23 | Positive |
| SA051195 | Patient 21 | Positive |
| SA051196 | Patient 20 | Positive |
| SA051197 | Patient 19 | Positive |
| SA051198 | Patient 22 | Positive |
| SA051199 | Patient 24 | Positive |
| SA051200 | Patient 26 | Positive |
| SA051201 | Patient 25 | Positive |
| SA051202 | Patient 27 | Positive |
| Showing results 1 to 35 of 35 |
Collection:
| Collection ID: | CO000911 |
| Collection Summary: | Breath collection was performed as previously reported with alterations detailed here (see PMID: 25810441). In brief, ≥ 1 L of exhaled breath was collected in a 3 L SamplePro Flexfilm sample bag (SKC Inc.). Using a set flow pump (ACTI-VOC, Markes International), exactly 1 L of breath was pumped through an inert stainless-steel sorbent tube with a triple bed sorbent composed of Tenax 60/80/ Carbograph 1 60/80/ Carboxen 1003 40/60 (Camsco). Sorbent tubes were stored at -20°C prior to shipment on black ice for later mass spectrometric analysis. |
| Sample Type: | Breath |
Treatment:
| Treatment ID: | TR000931 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP000924 |
| Sampleprep Summary: | N/A |
Chromatography:
| Chromatography ID: | CH001011 |
| Chromatography Summary: | All samples were run with a TurboMatrix 650 ATD (Perkin Elmer) connected to a Leco Pegasus 4D GCxGC-TOFMS system. Before analysis, sorbent tubes were brought to room temperature and purged for 5 min with BiP N2 (Airgas) at 60 mL/min. A gaseous standard mixture (20.1 ng fluorobenzene, 18.6 ng toluene-D8, 21.7 ng bromofluorobenzene, 20.3 ng 1,2-dichlorobenzene-D4) was added to each tube by the TurboMatrix 650 immediately prior to analysis. Tubes were desorbed at 270°C, 40 mL/min He flow, with recollection on a secondary Tenax cold trap at 10°C. Analytes were released from the secondary trap by heating to 295°C with 20% transferred to the GC/MS. The GC had a 30 m length x 0.25 mm ID x 0.25 µm film thickness DB-5 column (Agilent). The GC oven was programmed to hold at 40°C for 3 min, ramp 5°C/min to 200°C, then ramp 10°C/min to 250°C, final ramp 25°C/min to 300°C, then hold at 300°C for 3 min. The TOFMS had a sampling frequency of 50 Hz and a mass recording range of 34-400 amu. |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Flow Rate: | 1.2 mL/min |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN001440 |
| Analysis Type: | MS |
| Chromatography ID: | CH001011 |
| Num Factors: | 2 |
| Num Metabolites: | 147 |
| Units: | raw abundance |
