Summary of Study ST000922
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000638. The data can be accessed directly via it's Project DOI: 10.21228/M86M4G This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000922 |
| Study Title | Crab Urine Study |
| Study Summary | Metabolites from blue crab urine associated with different diets |
| Institute | Georgia Institute of Technology |
| Department | School of Chemistry and Biochemistry |
| Laboratory | SyMSC |
| Last Name | Gaul |
| First Name | David |
| Address | 311 Ferst Drive, Atlanta, GA, 30332, USA |
| david.gaul@chemistry.gatech.edu | |
| Phone | 4043850377 |
| Submit Date | 2017-12-14 |
| Num Groups | 2 |
| Total Subjects | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000638 |
| Project DOI: | doi: 10.21228/M86M4G |
| Project Title: | Metabolites associated with risk perception and predator detection |
| Project Summary: | Blue Crab fed either mud crab or oyster diet to investigator behavior changes of mud crabs due to compounds released in blue crab urine. |
| Institute: | Georgia Institute of Technology |
| Department: | School of Chemistry and Biochemistry |
| Laboratory: | Systems Mass Spectrometry Core (SyMSC) |
| Last Name: | Gaul |
| First Name: | David |
| Address: | 311 Ferst Drive, Atlanta, GA, 30332, USA |
| Email: | david.gaul@chemistry.gatech.edu |
| Phone: | 4043850377 |
| Funding Source: | NSF |
Subject:
| Subject ID: | SU000960 |
| Subject Type: | Animal |
| Subject Species: | Callinectes sapidus |
| Taxonomy ID: | 6763 |
| Species Group: | Invertebrates |
Factors:
Subject type: Animal; Subject species: Callinectes sapidus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA054697 | MA | mud crab diet |
| SA054698 | MD | mud crab diet |
| SA054699 | MC | mud crab diet |
| SA054700 | MB | mud crab diet |
| SA054701 | OD | oyster diet |
| SA054702 | OA | oyster diet |
| SA054703 | OB | oyster diet |
| SA054704 | OC | oyster diet |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO000954 |
| Collection Summary: | Urine was collected from Blue Crabs |
| Sample Type: | Urine |
| Collection Method: | 22-gauge needle with gentle suction |
| Collection Location: | Skidway Institute of Oceanography |
| Collection Frequency: | every other day |
| Storage Conditions: | -20C |
Treatment:
| Treatment ID: | TR000974 |
| Treatment Summary: | Blue crabs were fed mud crabs or oysters |
Sample Preparation:
| Sampleprep ID: | SP000967 |
| Sampleprep Summary: | Dilute and shoot, urine |
| Processing Method: | mixed 1 (sample): 5 (MeOH), centrifuged, supernatent analyzed |
| Processing Storage Conditions: | on ice |
Combined analysis:
| Analysis ID | AN001511 | AN001512 |
|---|---|---|
| Chromatography ID | CH001065 | CH001065 |
| MS ID | MS001394 | MS001395 |
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH Amide (75 x 2.1mm,1.7um) | Waters Acquity BEH Amide (75 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001065 |
| Chromatography Summary: | Basic pH HILIC method |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH Amide (75 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | (time, %A): 0, 5; 3, 63; 7, 63; 7.1, 5; 7.2, 0.5; 9.5, 5; 9.9,5. |
| Flow Rate: | (time, rate (ml/min)): 0, 0.3; 3, 0.3; 7, 0.3; 7.1, 0.3; 7.2, 0.5; 9.5, 0.5; 9.9, 0.3. |
| Sample Injection: | 2 ul |
| Solvent A: | 100% water; 10 mM sodium acetate; 0.05% ammonium hydroxide |
| Solvent B: | 100% acetonitrile; 0.05% ammonium hydroxide |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001394 |
| Analysis ID: | AN001511 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 275 C |
| Collision Energy: | 10, 30, 50 NCE stepped |
| Collision Gas: | nitrogen |
| Fragmentation Method: | HCD |
| Spray Voltage: | 3.00 kV |
| Automatic Gain Control: | 3.00E+6 |
| Resolution Setting: | 240,000 |
| Scanning Range: | 70-1050 |
| MS ID: | MS001395 |
| Analysis ID: | AN001512 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 275 C |
| Collision Energy: | 10, 30, 50 NCE stepped |
| Collision Gas: | nitrogen |
| Fragmentation Method: | HCD |
| Spray Voltage: | 2.80 kV |
| Automatic Gain Control: | 3.00E+6 |
| Resolution Setting: | 240,000 |
| Scanning Range: | 70-1050 |
