Summary of study ST000922

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000638. The data can be accessed directly via it's Project DOI: 10.21228/M86M4G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000922
Study TitleCrab Urine Study
Study SummaryMetabolites from blue crab urine associated with different diets
Institute
Georgia Institute of Technology
DepartmentSchool of Chemistry and Biochemistry
LaboratorySyMSC
Last NameGaul
First NameDavid
Address311 Ferst Drive, Atlanta, GA, 30332, USA
Emaildavid.gaul@chemistry.gatech.edu
Phone4043850377
Submit Date2017-12-14
Num Groups2
Total Subjects8
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
David Gaul David Gaul
https://dx.doi.org/10.21228/M86M4G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000638
Project DOI:doi: 10.21228/M86M4G
Project Title:Metabolites associated with risk perception and predator detection
Project Summary:Blue Crab fed either mud crab or oyster diet to investigator behavior changes of mud crabs due to compounds released in blue crab urine.
Institute:Georgia Institute of Technology
Department:School of Chemistry and Biochemistry
Laboratory:Systems Mass Spectrometry Core (SyMSC)
Last Name:Gaul
First Name:David
Address:311 Ferst Drive, Atlanta, GA, 30332, USA
Email:david.gaul@chemistry.gatech.edu
Phone:4043850377
Funding Source:NSF

Subject:

Subject ID:SU000960
Subject Type:Animal
Subject Species:Callinectus sapidus
Taxonomy ID:6763

Factors:

Subject type: Animal; Subject species: Callinectus sapidus (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA054697MAmud crab diet
SA054698MDmud crab diet
SA054699MCmud crab diet
SA054700MBmud crab diet
SA054701ODoyster diet
SA054702OAoyster diet
SA054703OBoyster diet
SA054704OCoyster diet
Showing results 1 to 8 of 8

Collection:

Collection ID:CO000954
Collection Summary:Urine was collected from Blue Crabs
Sample Type:Urine
Collection Method:22-gauge needle with gentle suction
Collection Location:Skidway Institute of Oceanography
Collection Frequency:every other day
Storage Conditions:-20C

Treatment:

Treatment ID:TR000974
Treatment Summary:Blue crabs were fed mud crabs or oysters

Sample Preparation:

Sampleprep ID:SP000967
Sampleprep Summary:Dilute and shoot, urine
Processing Method:mixed 1 (sample): 5 (MeOH), centrifuged, supernatent analyzed
Processing Storage Conditions:on ice

Combined analysis:

Analysis ID AN001511 AN001512
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters Acquity BEH Amide (75 x 2.1mm, 1.7um) Waters Acquity BEH Amide (75 x 2.1mm, 1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH001065
Chromatography Summary:Basic pH HILIC method
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH Amide (75 x 2.1mm, 1.7um)
Column Temperature:50
Flow Gradient:(time, %A): 0, 5; 3, 63; 7, 63; 7.1, 5; 7.2, 0.5; 9.5, 5; 9.9,5.
Flow Rate:(time, rate (ml/min)): 0, 0.3; 3, 0.3; 7, 0.3; 7.1, 0.3; 7.2, 0.5; 9.5, 0.5; 9.9, 0.3.
Sample Injection:2 ul
Solvent A:10mM NaOAc, 0,05% NH4OH
Solvent B:ACN, 0.05% NH4OH
Chromatography Type:HILIC

MS:

MS ID:MS001394
Analysis ID:AN001511
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:275 C
Collision Energy:10, 30, 50 NCE stepped
Collision Gas:nitrogen
Fragmentation Method:HCD
Spray Voltage:3.00 kV
Automatic Gain Control:3.00E+6
Resolution Setting:240,000
Scanning Range:70-1050
  
MS ID:MS001395
Analysis ID:AN001512
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:275 C
Collision Energy:10, 30, 50 NCE stepped
Collision Gas:nitrogen
Fragmentation Method:HCD
Spray Voltage:2.80 kV
Automatic Gain Control:3.00E+6
Resolution Setting:240,000
Scanning Range:70-1050
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