Summary of Study ST000940

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000940
Study TitleActivity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv3285
Study Typetimecourse
Study SummaryThese experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.
Institute
Weill Cornell Medicine
Last NameRhee
First NameKyu
AddressDepartment of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY
Emailkyr9001@med.cornell.edu
Phone979-229-1494
Submit Date2018-03-07
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Kyu Rhee Kyu Rhee
https://dx.doi.org/10.21228/M8388M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000187
Project DOI:doi: 10.21228/M8388M
Project Title:FLUTE: Functionalizing Lists of Unknown TB Entities
Project Type:NIH U19
Project Summary:functional genomics of proteins of unknown function in M. tuberculosis
Institute:Weill Cornell Medicine
Department:Microbiology and Immunology
Last Name:Rhee
First Name:Kyu
Address:#N/A
Email:kyr9001@med.cornell.edu

Subject:

Subject ID:SU000979
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Species Group:Bacteria

Factors:

Subject type: Bacteria; Subject species: Mycobacterium tuberculosis H37Rv (Factor headings shown in green)

mb_sample_id local_sample_id Time Protein
SA0559133285_B_1515 Active
SA0559143285_A_1515 Active
SA0559153285_C_1515 Active
SA055916Inactivated-3285_C_1515 Inactivated
SA055917Inactivated-3285_B_1515 Inactivated
SA055918Inactivated-3285_A_1515 Inactivated
SA0559193285_C_3030 Active
SA0559203285_B_3030 Active
SA0559213285_A_3030 Active
SA055922Inactivated-3285_C_3030 Inactivated
SA055923Inactivated-3285_A_3030 Inactivated
SA055924Inactivated-3285_B_3030 Inactivated
SA0559073285_A_0- Active
SA0559083285_C_0- Active
SA0559093285_B_0- Active
SA055910Inactivated-3285_B_0- Inactivated
SA055911Inactivated-3285_C_0- Inactivated
SA055912Inactivated-3285_A_0- Inactivated
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000973
Collection Summary:Rv3285 from M. tuberculosis H37Rv was expressed in E. coli cells and purified.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000993
Treatment Summary:Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated).

Sample Preparation:

Sampleprep ID:SP000986
Sampleprep Summary:Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated).

Chromatography:

Chromatography ID:CH001083
Chromatography Summary:Aqueous normal phase diamond hydride
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Chromatography Type:Normal phase

Analysis:

Analysis ID:AN001541
Analysis Type:MS
Instrument Name:Agilent 6220 TOF
Chromatography ID:CH001083
Num Factors:6
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST000940_AN001541_Results.txt
Units:peak counts
  
Analysis ID:AN001542
Analysis Type:MS
Instrument Name:Agilent 6220 TOF
Chromatography ID:CH001083
Num Factors:6
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST000940_AN001542_Results.txt
Units:peak counts
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