Summary of Study ST000944
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000649. The data can be accessed directly via it's Project DOI: 10.21228/M8SD52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000944 |
Study Title | Amino Acids, Acylcarnitine, & Insulin for P20 Participants |
Study Type | MS analysis |
Study Summary | See attached document. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2018-03-09 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Chear Study | No |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2020-09-12 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000649 |
Project DOI: | doi: 10.21228/M8SD52 |
Project Title: | Metabolomic profiles and development of metabolic risk during puberty in the ELEMENT cohort |
Project Type: | MS analysis |
Project Summary: | Prospective investigation of amino acids and acylcarnitine metabolites with change in metabolic biomarkers during the pubertal transition among 208 peripubertal youth. |
Institute: | University of Michigan |
Department: | Nutritional Sciences |
Laboratory: | Peterson Lab |
Last Name: | Perng |
First Name: | Wei |
Address: | Ann Arbor, MI |
Email: | perngwei@umich.edu |
Phone: | 734-764-7594 |
Subject:
Subject ID: | SU000983 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | SampleType |
---|---|---|
SA055979 | S00027034 | Blinded |
SA055980 | S00027033 | Blinded |
SA055981 | S00027035 | Blinded |
SA055982 | S00027036 | Blinded |
SA055983 | S00027037 | Blinded |
SA055984 | S00027032 | Blinded |
SA055985 | S00027031 | Blinded |
SA055986 | S00027027 | Blinded |
SA055987 | S00027028 | Blinded |
SA055988 | S00027029 | Blinded |
SA055989 | S00027030 | Blinded |
SA055990 | S00027038 | Blinded |
SA055991 | S00027040 | Blinded |
SA055992 | S00027047 | Blinded |
SA055993 | S00027046 | Blinded |
SA055994 | S00027048 | Blinded |
SA055995 | S00027049 | Blinded |
SA055996 | S00027050 | Blinded |
SA055997 | S00027045 | Blinded |
SA055998 | S00027044 | Blinded |
SA055999 | S00027026 | Blinded |
SA056000 | S00027041 | Blinded |
SA056001 | S00027042 | Blinded |
SA056002 | S00027043 | Blinded |
SA056003 | S00027039 | Blinded |
SA056004 | S00027024 | Blinded |
SA056005 | S00027008 | Blinded |
SA056006 | S00027007 | Blinded |
SA056007 | S00027009 | Blinded |
SA056008 | S00027010 | Blinded |
SA056009 | S00027011 | Blinded |
SA056010 | S00027006 | Blinded |
SA056011 | S00027005 | Blinded |
SA056012 | S00027001 | Blinded |
SA056013 | S00027002 | Blinded |
SA056014 | S00027003 | Blinded |
SA056015 | S00027004 | Blinded |
SA056016 | S00027012 | Blinded |
SA056017 | S00027013 | Blinded |
SA056018 | S00027021 | Blinded |
SA056019 | S00027020 | Blinded |
SA056020 | S00027022 | Blinded |
SA056021 | S00027023 | Blinded |
SA056022 | S00027051 | Blinded |
SA056023 | S00027019 | Blinded |
SA056024 | S00027018 | Blinded |
SA056025 | S00027014 | Blinded |
SA056026 | S00027015 | Blinded |
SA056027 | S00027016 | Blinded |
SA056028 | S00027017 | Blinded |
SA056029 | S00027025 | Blinded |
SA056030 | S00027053 | Blinded |
SA056031 | S00027087 | Blinded |
SA056032 | S00027086 | Blinded |
SA056033 | S00027088 | Blinded |
SA056034 | S00027089 | Blinded |
SA056035 | S00027090 | Blinded |
SA056036 | S00027085 | Blinded |
SA056037 | S00027084 | Blinded |
SA056038 | S00027080 | Blinded |
SA056039 | S00027081 | Blinded |
SA056040 | S00027082 | Blinded |
SA056041 | S00027083 | Blinded |
SA056042 | S00027091 | Blinded |
SA056043 | S00027092 | Blinded |
SA056044 | S00027100 | Blinded |
SA056045 | S00027099 | Blinded |
SA056046 | S00027101 | Blinded |
SA056047 | S00027102 | Blinded |
SA056048 | S00027064 | Blinded |
SA056049 | S00027098 | Blinded |
SA056050 | S00027097 | Blinded |
SA056051 | S00027093 | Blinded |
SA056052 | S00027094 | Blinded |
SA056053 | S00027095 | Blinded |
SA056054 | S00027096 | Blinded |
SA056055 | S00027079 | Blinded |
SA056056 | S00027078 | Blinded |
SA056057 | S00027060 | Blinded |
SA056058 | S00027059 | Blinded |
SA056059 | S00027061 | Blinded |
SA056060 | S00027062 | Blinded |
SA056061 | S00027063 | Blinded |
SA056062 | S00027058 | Blinded |
SA056063 | S00027057 | Blinded |
SA056064 | S00027000 | Blinded |
SA056065 | S00027054 | Blinded |
SA056066 | S00027055 | Blinded |
SA056067 | S00027056 | Blinded |
SA056068 | S00027065 | Blinded |
SA056069 | S00027066 | Blinded |
SA056070 | S00027074 | Blinded |
SA056071 | S00027073 | Blinded |
SA056072 | S00027075 | Blinded |
SA056073 | S00027076 | Blinded |
SA056074 | S00027077 | Blinded |
SA056075 | S00027072 | Blinded |
SA056076 | S00027071 | Blinded |
SA056077 | S00027067 | Blinded |
SA056078 | S00027068 | Blinded |
Collection:
Collection ID: | CO000977 |
Collection Summary: | None |
Sample Type: | Plasma;Feces;Cells;Tissue |
Treatment:
Treatment ID: | TR000997 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP000990 |
Sampleprep Summary: | Amino acids are analyzed using the Phenomenex EZfaast kit. Samples are extracted, semi-purified, derivitized and measured by EI-GCMS using norvaline as an internal standard for normalization. See reference for User Guide and analytical details. Analytes are reported as uM with CV's generally 10%, but Histidine is exceptional with a CV ~30 |
Sampleprep Protocol Filename: | Amino_acids_analysis.pdf |
Combined analysis:
Analysis ID | AN001549 | AN001550 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | GC | Reversed phase |
Chromatography system | Agilent 7890N | Agilent 6490A |
Column | Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
MS Type | EI | ESI |
MS instrument type | Single quadrupole | Triple quadrupole |
MS instrument name | Agilent | Agilent 6490A QQQ |
Ion Mode | POSITIVE | POSITIVE |
Units | uM | uM |
Chromatography:
Chromatography ID: | CH001087 |
Chromatography Summary: | None |
Methods Filename: | EZGCMS_1.M |
Instrument Name: | Agilent 7890N |
Column Name: | Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex) |
Chromatography Type: | GC |
Chromatography ID: | CH001088 |
Methods Filename: | QM-004-HSS_50mm_ACar+_MRM-Insert-gradtest3 |
Instrument Name: | Agilent 6490A |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001428 |
Analysis ID: | AN001549 |
Instrument Name: | Agilent |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
MS ID: | MS001429 |
Analysis ID: | AN001550 |
Instrument Name: | Agilent 6490A QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |