Summary of study ST000953

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000654. The data can be accessed directly via it's Project DOI: 10.21228/M84M4V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000953
Study TitleIdentification of putative cryoprotectant metabolites in Aphaenogaster picea
Study Typethree treatments (control, cold shock, cold acclimation)
Study SummaryQuantification of metabolite abundances produced in response to acute or chronic cold stress (4 replicates for each of three treatments)
Institute
University of Florida
DepartmentSECIM
Last NameCahan
First NameSara
AddressDepartment of Biology, Univ. of VT, Burlington, VT 05405
Emailscahan@uvm.edu
Phone8026562962
Submit Date2018-04-11
Num Groups3
Total Subjects12
Study CommentsSECIM pilot and feasibility, NIH U24 DK097209 (if applicable)
Raw Data AvailableYes
Raw Data File Type(s).raw, .RST
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Sara Cahan Sara Cahan
https://dx.doi.org/10.21228/M84M4V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000654
Project DOI:doi: 10.21228/M84M4V
Project Title:The effect of a trehalose diet on cold tolerance in Apheanogaster picea
Project Summary:Investigation of physiological resistance to low temperatures in a northern freeze-avoidant ant species.
Institute:University of Vermont
Department:Biology
Last Name:Cahan
First Name:Sara
Address:Department of Biology, Univ. of VT, Burlington, VT 05405
Email:scahan@uvm.edu
Phone:(802)656-2962

Subject:

Subject ID:SU000992
Subject Type:Insect
Subject Species:Aphaenogaster picea
Taxonomy ID:602698
Genotype Strain:Field colonies of forest ant

Factors:

Subject type: Insect; Subject species: Aphaenogaster picea (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA057136EW-06-ACCACC
SA057137EW-02-ACCACC
SA057138EW-10-ACCACC
SA057139EW-07-ACCACC
SA057140EW-07-RCHRCH
SA057141EW-06-RCHRCH
SA057142EW-02-RCHRCH
SA057143EW-10-RCHRCH
SA057144EW-07-RTRT
SA057145EW-06-RTRT
SA057146EW-02-RTRT
SA057147EW-10-RTRT
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000986
Collection Summary:Live animals flash-frozen in liquid nitrogen and kept at -80. Sample source is whole animal tissue (~40 workers/sample)
Sample Type:Whole Tissue

Treatment:

Treatment ID:TR001006
Treatment Summary:Control - at 25 degrees C. Cold shock - 0 degrees C for one hour, recovery at 25 degrees one hour. Acclimation - two weeks at 4 degrees C. Acronyms used in study design for treatment (ACC - acclimation, RCH - cold shock, RT - room temperature)

Sample Preparation:

Sampleprep ID:SP000999
Sampleprep Summary:none
Sampleprep Protocol Filename:GMetabolomics_LCMS_Protocol_092117.pdf
Appendix_A_Internal_Standard_Prep_GLCMS.pdf
Sample Spiking:Appendix_A_Internal_Standard_Prep_GLCMS.pdf

Combined analysis:

Analysis ID AN001562 AN001563
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Height peak height

Chromatography:

Chromatography ID:CH001096
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:350 ul/min
Solvent A:0.1% Formic Acid in Water
Solvent B:Acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001440
Analysis ID:AN001562
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001441
Analysis ID:AN001563
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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