Summary of Study ST000982

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000671. The data can be accessed directly via it's Project DOI: 10.21228/M8XX0K This work is supported by NIH grant, U2C- DK119886.

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Study IDST000982
Study TitleAnalysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala.
Study SummaryAnalysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala by HPLC-MS/MS.
Institute
Huazhong Agricultural University
Last NameXu
First NameJia
AddressLion rock street, hung shan district, Wuhan, HuBei, 430070, China
Emailxujia2018hzau@163.com
Phone13018097215
Submit Date2018-06-25
Analysis Type DetailLC-MS
Release Date2018-08-27
Release Version1
Jia Xu Jia Xu
https://dx.doi.org/10.21228/M8XX0K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000671
Project DOI:doi: 10.21228/M8XX0K
Project Title:Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala.
Project Summary:Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephalab by HPLC-MS/MS
Institute:Huazhong Agricultural University
Last Name:Xu
First Name:Jia
Address:Lion rock street, hung shan district, Wuhan, HuBei, 430070, China
Email:xujia2018hzau@163.com
Phone:13018097215

Subject:

Subject ID:SU001021
Subject Type:Fish
Subject Species:Megalobrama amblycephala
Taxonomy ID:75352
Species Group:Fish

Factors:

Subject type: Fish; Subject species: Megalobrama amblycephala (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA06003615Betaine supplementary for 16 weeks
SA06003716Betaine supplementary for 16 weeks
SA06003818Betaine supplementary for 16 weeks
SA06003914Betaine supplementary for 16 weeks
SA06004017Betaine supplementary for 16 weeks
SA06004113Betaine supplementary for 16 weeks
SA06004220Betaine supplementary for 4 weeks
SA06004319Betaine supplementary for 4 weeks
SA06004421Betaine supplementary for 4 weeks
SA06004522Betaine supplementary for 4 weeks
SA06004623Betaine supplementary for 4 weeks
SA06004724Betaine supplementary for 4 weeks
SA0600483Control group
SA0600492Control group
SA0600504Control group
SA0600515Control group
SA0600526Control group
SA0600531Control group
SA06005410High carbohydrates group
SA0600559High carbohydrates group
SA06005611High carbohydrates group
SA06005712High carbohydrates group
SA0600588High carbohydrates group
SA0600597High carbohydrates group
Showing results 1 to 24 of 24

Collection:

Collection ID:CO001015
Collection Summary:After 16 weeks of the feeding trial, approximately 24h after the last feeding, fish in all tanks were anaesthetized with MS-222 (Sigma Aldrich, USA) at the concentration of 100 mg L-1. Fifteen specimens from each tank were selected randomly to obtain the blood. Blood was obtained from the caudal vein using sterile syringes with pre-added anticoagulant solution and then centrifuged (3000×g) at 4°C for 10 min to obtain the serum, which was quickly frozen in liquid nitrogen and stored at -80°C for biochemical assays.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR001035
Treatment Summary:Control diet (CD group) was formulated to contain 27.11±0.38% carbohydrates. The high-carbohydrate diet (HCD group) was formulated to contain over 36.75±0.92% of easily digestible carbohydrates (mostly from flour). The betaine treatment diet (HCB) to contain a high level of carbohydrates (35.64±0.43%) with 1% betaine supplemented for 16 weeks. HC4B group was fed a combination of HCD (first twelve weeks) and HCB diets (last four weeks).
Treatment Protocol Filename:Experiment_methods.docx

Sample Preparation:

Sampleprep ID:SP001028
Sampleprep Summary:Fifty μl of protein precipitant (containing NVL) was added to 50 μl of serum solution of each sample, mixed thoroughly, and centrifuged at 13200 rpm for 4 min. The supernatant (10 μl) was mixed with 50 μl methanol borate buffer (0.1 M, pH=8.8) thoroughly. For amino acids, the above solution was mixed with 20 μl of diethyl ethoxymethylene-malonate at 55°C for 15 min.

Chromatography:

Chromatography ID:CH001130
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Unspecified
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN001608
Analysis Type:MS
Instrument Name:API 3200Q TRAP
Chromatography ID:CH001130
Num Factors:4
Num Metabolites:16
Units:umol/L or ng/mL
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