Summary of Study ST001049

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000703. The data can be accessed directly via it's Project DOI: 10.21228/M8T10P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001049
Study TitleP4HA1 knockdown in the breast cell line MDA231 (part I)
Study Typeisotope tracer
Study SummaryDetermine the effect of knocking down P4HA1 in the MDA231 cell line (including alterations to metabolic pathways). Control versus knockdown
Institute
University of Kentucky
DepartmentMarkey Cancer Center
Last NameXiong
First NameGaofeng
AddressBBSRC361, 741 South Limestone, Lexington, KY 40536, USA
Emailgaofeng.xiong@uky.edu
Phone000-000-0000
Submit Date2018-09-12
Study CommentsFor additional comments about the study, like listing a publication.
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2019-03-06
Release Version1
Gaofeng Xiong Gaofeng Xiong
https://dx.doi.org/10.21228/M8T10P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000703
Project DOI:doi: 10.21228/M8T10P
Project Title:P4HA1 knockdown in the breast cell line MDA231
Project Summary:Determine the effect of knocking down P4HA1 in the MDA231 cell line (including alterations to metabolic pathways).
Institute:University of Kentucky
Department:Markey Cancer Center
Last Name:Xu
First Name:Ren
Address:BBSRC361, 741 South Limestone, Lexington, KY 40536, USA
Email:ren.xu2010@uky.edu
Phone:000-000-0000

Subject:

Subject ID:SU001198
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id protocol.id
SA0782611_MDA231_12CGlc_shCTL_rep1-cells-polar_GCMS_A12CGlc_shCTL
SA0782624_MDA231_12CGlc_shP4HA1_rep1-cells-polar_GCMS_A12CGlc_shP4HA1
SA0782633_MDA231_13CGlc_shCTL_rep2-cells-polar_GCMS_A13CGlc_shCTL
SA0782642_MDA231_13CGlc_shCTL_rep1-cells-polar_GCMS_A13CGlc_shCTL
SA0782655_MDA231_13CGlc_shP4HA1_rep1-cells-polar_GCMS_A13CGlc_shP4HA1
SA0782666_MDA231_13CGlc_shP4HA1_rep2-cells-polar_GCMS_A13CGlc_shP4HA1
Showing results 1 to 6 of 6

Collection:

Collection ID:CO001192
Collection Summary:Collection and quench of cells in culture
Collection Protocol ID:cell_quench_collection
Collection Protocol Filename:3A_Cells_Quench_Cell_Fan_20170412.pdf
Sample Type:cultured cells

Treatment:

Treatment ID:TR001213
Treatment Summary:Cells grown in unlabeled glucose and infected with empty vector. Cells grown in unlabeled glucose and infected with shP4HA1 lentivirus. Cells grown in labeled glucose and infected with empty vector. Cells grown in labeled glucose and infected with shP4HA1 lentivirus.
Treatment Protocol ID:12CGlc_shCTL 12CGlc_shP4HA1 13CGlc_shCTL 13CGlc_shP4HA1

Sample Preparation:

Sampleprep ID:SP001206
Sampleprep Summary:Polar extraction from cells. Protein extraction and quantification.
Sampleprep Protocol ID:polar_extraction protein_extraction
Sampleprep Protocol Filename:4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf
4D_17Jun4_Fan_Prot_Quant.pdf

Combined analysis:

Analysis ID AN001864
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units normalized peak area

Chromatography:

Chromatography ID:CH001349
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

MS:

MS ID:MS001724
Analysis ID:AN001864
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).
Ion Mode:POSITIVE
  logo