Summary of Study ST001063
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000713. The data can be accessed directly via it's Project DOI: 10.21228/M8HM48 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001063 |
| Study Title | Lipidomics analysis for aged mice epididymal adipose tissue (part-I) |
| Study Type | MS lipidomics analysis |
| Study Summary | Lipidomics studies on samples from aged and young mice. |
| Institute | Takeda Pharmaceutical Company Limited |
| Last Name | Ando |
| First Name | Ayumi |
| Address | 26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa 251-8555, Japan |
| ayumi.kawamura@takeda.com | |
| Phone | +81466-32-4216 |
| Submit Date | 2018-09-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | gda |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-10-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000713 |
| Project DOI: | doi: 10.21228/M8HM48 |
| Project Title: | Lipidomics analysis for aged mice organs |
| Project Type: | MS lipidomics analysis |
| Project Summary: | Lipidomics analysis for aged mice organs |
| Institute: | Takeda Pharmaceutical Company Limited |
| Last Name: | Ando |
| First Name: | Ayumi |
| Address: | 26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa, 251-8555, Japan |
| Email: | ayumi.kawamura@takeda.com |
| Phone: | +81-466-32-4216 |
Subject:
| Subject ID: | SU001107 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J mice |
| Age Or Age Range: | 8 week (“young”) and 112 week (“old”). |
| Weight Or Weight Range: | young: 20.2g (mean), aged: 34.3g (mean) |
| Gender: | Male |
| Animal Animal Supplier: | CLEA (Tokyo, Japan) |
| Animal Light Cycle: | a 12-hour light/12-hour dark schedule |
| Animal Feed: | CE-2, CLEA |
| Animal Water: | tap water |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Age | Tissue |
|---|---|---|---|
| SA071883 | Cortex_60_aged | Aged 112 weeks | Cerebral Cortex |
| SA071884 | Cortex_58_aged | Aged 112 weeks | Cerebral Cortex |
| SA071885 | Cortex_56_aged | Aged 112 weeks | Cerebral Cortex |
| SA071886 | Cortex_54_aged | Aged 112 weeks | Cerebral Cortex |
| SA071887 | Cortex_52_aged | Aged 112 weeks | Cerebral Cortex |
| SA071888 | Adipose_60_aged | Aged 112 weeks | Epididymal adipose tissue |
| SA071889 | Adipose_58_aged | Aged 112 weeks | Epididymal adipose tissue |
| SA071890 | Adipose_52_aged | Aged 112 weeks | Epididymal adipose tissue |
| SA071891 | Adipose_54_aged | Aged 112 weeks | Epididymal adipose tissue |
| SA071892 | Adipose_56_aged | Aged 112 weeks | Epididymal adipose tissue |
| SA071893 | Femoral_52_aged | Aged 112 weeks | Femoral muscle |
| SA071894 | Femoral_58_aged | Aged 112 weeks | Femoral muscle |
| SA071895 | Femoral_60_aged | Aged 112 weeks | Femoral muscle |
| SA071896 | Femoral_56_aged | Aged 112 weeks | Femoral muscle |
| SA071897 | Femoral_54_aged | Aged 112 weeks | Femoral muscle |
| SA071898 | Liver_60_aged | Aged 112 weeks | Liver |
| SA071899 | Liver_58_aged | Aged 112 weeks | Liver |
| SA071900 | Liver_56_aged | Aged 112 weeks | Liver |
| SA071901 | Liver_52_aged | Aged 112 weeks | Liver |
| SA071902 | Liver_54_aged | Aged 112 weeks | Liver |
| SA071903 | Cortex_59_young | Young 8 weeks | Cerebral Cortex |
| SA071904 | Cortex_57_young | Young 8 weeks | Cerebral Cortex |
| SA071905 | Cortex_51_young | Young 8 weeks | Cerebral Cortex |
| SA071906 | Cortex_55_young | Young 8 weeks | Cerebral Cortex |
| SA071907 | Cortex_53_young | Young 8 weeks | Cerebral Cortex |
| SA071908 | Adipose_59_young | Young 8 weeks | Epididymal adipose tissue |
| SA071909 | Adipose_57_young | Young 8 weeks | Epididymal adipose tissue |
| SA071910 | Adipose_55_young | Young 8 weeks | Epididymal adipose tissue |
| SA071911 | Adipose_51_young | Young 8 weeks | Epididymal adipose tissue |
| SA071912 | Adipose_53_young | Young 8 weeks | Epididymal adipose tissue |
| SA071913 | Femoral_57_young | Young 8 weeks | Femoral muscle |
| SA071914 | Femoral_59_young | Young 8 weeks | Femoral muscle |
| SA071915 | Femoral_55_young | Young 8 weeks | Femoral muscle |
| SA071916 | Femoral_53_young | Young 8 weeks | Femoral muscle |
| SA071917 | Femoral_51_young | Young 8 weeks | Femoral muscle |
| SA071918 | Liver_59_young | Young 8 weeks | Liver |
| SA071919 | Liver_57_young | Young 8 weeks | Liver |
| SA071920 | Liver_55_young | Young 8 weeks | Liver |
| SA071921 | Liver_53_young | Young 8 weeks | Liver |
| SA071922 | Liver_51_young | Young 8 weeks | Liver |
| Showing results 1 to 40 of 40 |
Collection:
| Collection ID: | CO001101 |
| Collection Summary: | All mice were killed by decapitation for sample collection. Collected tissues were immediately frozen by liquid nitrogen then stored -80 C until use. (Femoral, Cortex, Liver, Adipose) tissues. |
| Sample Type: | Adipose tissue |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001121 |
| Treatment Summary: | Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics. |
Sample Preparation:
| Sampleprep ID: | SP001114 |
| Sampleprep Summary: | Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics. |
Combined analysis:
| Analysis ID | AN001739 | AN001740 |
|---|---|---|
| Chromatography ID | CH001230 | CH001230 |
| MS ID | MS001608 | MS001609 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Orbitrap XL | Orbitrap XL |
| Column | Waters XBridge C18 (50 x 2.1mm,3um) | Waters XBridge C18 (50 x 2.1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo LTQ XL | Thermo LTQ XL |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001230 |
| Chromatography Summary: | The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1]) |
| Instrument Name: | Orbitrap XL |
| Column Name: | Waters XBridge C18 (50 x 2.1mm,3um) |
| Solvent A: | 100% water; 0.01% acetic acid; 1 mM ammonia |
| Solvent B: | 75% ethanol/25% isopropanol; 0.001% acetic acid; 0.2 mM ammonia |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001608 |
| Analysis ID: | AN001739 |
| Instrument Name: | Thermo LTQ XL |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001609 |
| Analysis ID: | AN001740 |
| Instrument Name: | Thermo LTQ XL |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
