Summary of study ST001063

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000713. The data can be accessed directly via it's Project DOI: 10.21228/M8HM48 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001063
Study TitleLipidomics analysis for aged mice epididymal adipose tissue (part-I)
Study TypeMS lipidomics analysis
Study SummaryLipidomics studies on samples from aged and young mice.
Institute
Takeda Pharmaceutical Company Limited
Last NameAndo
First NameAyumi
Address26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa 251-8555, Japan
Emailayumi.kawamura@takeda.com
Phone+81466-32-4216
Submit Date2018-09-18
Raw Data AvailableYes
Raw Data File Type(s).gda
Analysis Type DetailLC-MS
Release Date2019-10-11
Release Version1
Ayumi Ando Ayumi Ando
https://dx.doi.org/10.21228/M8HM48
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000713
Project DOI:doi: 10.21228/M8HM48
Project Title:Lipidomics analysis for aged mice organs
Project Type:MS lipidomics analysis
Project Summary:Lipidomics analysis for aged mice organs
Institute:Takeda Pharmaceutical Company Limited
Last Name:Ando
First Name:Ayumi
Address:26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa, 251-8555, Japan
Email:ayumi.kawamura@takeda.com
Phone:+81-466-32-4216

Subject:

Subject ID:SU001107
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J mice
Age Or Age Range:8 week (“young”) and 112 week (“old”).
Weight Or Weight Range:young: 20.2g (mean), aged: 34.3g (mean)
Gender:Male
Animal Animal Supplier:CLEA (Tokyo, Japan)
Animal Light Cycle:a 12-hour light/12-hour dark schedule
Animal Feed:CE-2, CLEA
Animal Water:tap water

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Age Tissue
SA071883Cortex_60_agedAged 112 weeks Cerebral Cortex
SA071884Cortex_58_agedAged 112 weeks Cerebral Cortex
SA071885Cortex_56_agedAged 112 weeks Cerebral Cortex
SA071886Cortex_54_agedAged 112 weeks Cerebral Cortex
SA071887Cortex_52_agedAged 112 weeks Cerebral Cortex
SA071888Adipose_60_agedAged 112 weeks Epididymal adipose tissue
SA071889Adipose_58_agedAged 112 weeks Epididymal adipose tissue
SA071890Adipose_52_agedAged 112 weeks Epididymal adipose tissue
SA071891Adipose_54_agedAged 112 weeks Epididymal adipose tissue
SA071892Adipose_56_agedAged 112 weeks Epididymal adipose tissue
SA071893Femoral_52_agedAged 112 weeks Femoral muscle
SA071894Femoral_58_agedAged 112 weeks Femoral muscle
SA071895Femoral_60_agedAged 112 weeks Femoral muscle
SA071896Femoral_56_agedAged 112 weeks Femoral muscle
SA071897Femoral_54_agedAged 112 weeks Femoral muscle
SA071898Liver_60_agedAged 112 weeks Liver
SA071899Liver_58_agedAged 112 weeks Liver
SA071900Liver_56_agedAged 112 weeks Liver
SA071901Liver_52_agedAged 112 weeks Liver
SA071902Liver_54_agedAged 112 weeks Liver
SA071903Cortex_59_youngYoung 8 weeks Cerebral Cortex
SA071904Cortex_57_youngYoung 8 weeks Cerebral Cortex
SA071905Cortex_51_youngYoung 8 weeks Cerebral Cortex
SA071906Cortex_55_youngYoung 8 weeks Cerebral Cortex
SA071907Cortex_53_youngYoung 8 weeks Cerebral Cortex
SA071908Adipose_59_youngYoung 8 weeks Epididymal adipose tissue
SA071909Adipose_57_youngYoung 8 weeks Epididymal adipose tissue
SA071910Adipose_55_youngYoung 8 weeks Epididymal adipose tissue
SA071911Adipose_51_youngYoung 8 weeks Epididymal adipose tissue
SA071912Adipose_53_youngYoung 8 weeks Epididymal adipose tissue
SA071913Femoral_57_youngYoung 8 weeks Femoral muscle
SA071914Femoral_59_youngYoung 8 weeks Femoral muscle
SA071915Femoral_55_youngYoung 8 weeks Femoral muscle
SA071916Femoral_53_youngYoung 8 weeks Femoral muscle
SA071917Femoral_51_youngYoung 8 weeks Femoral muscle
SA071918Liver_59_youngYoung 8 weeks Liver
SA071919Liver_57_youngYoung 8 weeks Liver
SA071920Liver_55_youngYoung 8 weeks Liver
SA071921Liver_53_youngYoung 8 weeks Liver
SA071922Liver_51_youngYoung 8 weeks Liver
Showing results 1 to 40 of 40

Collection:

Collection ID:CO001101
Collection Summary:All mice were killed by decapitation for sample collection. Collected tissues were immediately frozen by liquid nitrogen then stored -80 C until use. (Femoral, Cortex, Liver, Adipose) tissues.
Sample Type:Adipose tissue
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001121
Treatment Summary:Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics.

Sample Preparation:

Sampleprep ID:SP001114
Sampleprep Summary:Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics.

Combined analysis:

Analysis ID AN001739 AN001740
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Orbitrap XL Orbitrap XL
Column Waters XBridge C18 (50 x 2.1mm, 3um) Waters XBridge C18 (50 x 2.1mm, 3um)
MS Type ESI ESI
MS instrument type Single quadrupole Single quadrupole
MS instrument name Thermo LTQ XL Thermo LTQ XL
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH001230
Chromatography Summary:The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1])
Instrument Name:Orbitrap XL
Column Name:Waters XBridge C18 (50 x 2.1mm, 3um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001608
Analysis ID:AN001739
Instrument Name:Thermo LTQ XL
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001609
Analysis ID:AN001740
Instrument Name:Thermo LTQ XL
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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