Summary of Study ST001076

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000721. The data can be accessed directly via it's Project DOI: 10.21228/M8GM4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001076
Study TitleMulti-platform metabolomics data from Ndufs4-/- skeletal muscles (part - I)
Study TypeTargeted LC-MS/MS of Ndufs4-/- white quadriceps muscles
Study SummaryTargeted LC-MS/MS analysis of amino acids and acylcarnitines in Ndufs4-/- and WT mouse white quadriceps muscles.
Institute
North-West University
Last NameLouw
First NameRoan
AddressHofman Street
EmailRoan.Louw@nwu.ac.za
Phone+27 18 299 4074
Submit Date2018-10-17
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Roan Louw Roan Louw
https://dx.doi.org/10.21228/M8GM4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000721
Project DOI:doi: 10.21228/M8GM4Z
Project Title:Multi-platform metabolomics data from Ndufs4-/- skeletal muscles
Project Type:Multi-platform metabolomics study
Project Summary:Multi-platform metabolomics analysis of white quadriceps and soleus skeletal muscles from the Ndufs4 knockout (Ndufs4-/-) mouse model of human Leigh syndrome.
Institute:North-West University
Last Name:Louw
First Name:Roan
Address:Hofman Street
Email:Roan.Louw@nwu.ac.za
Phone:+27 18 299 4074

Subject:

Subject ID:SU001120
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Ndufs4, https://www.jax.org/strai n/02705 8
Age Or Age Range:45-50 days
Gender:Male
Animal Animal Supplier:Jackson Laboratory (ME, USA)
Animal Light Cycle:12:12 h
Animal Feed:Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub
Animal Water:ad libitum
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA072523MQ_14Ndufs4-/-
SA072524MQ_17Ndufs4-/-
SA072525MQ_19Ndufs4-/-
SA072526MQ_12Ndufs4-/-
SA072527MQ_8Ndufs4-/-
SA072528MQ_4Ndufs4-/-
SA072529MQ_5Ndufs4-/-
SA072530MQ_21Ndufs4-/-
SA072531MQ_10Ndufs4-/-
SA072532MQ_24Ndufs4-/-
SA072533MQ_37Ndufs4-/-
SA072534MQ_38Ndufs4-/-
SA072535MQ_40Ndufs4-/-
SA072536MQ_35Ndufs4-/-
SA072537MQ_34Ndufs4-/-
SA072538MQ_2Ndufs4-/-
SA072539MQ_26Ndufs4-/-
SA072540MQ_29Ndufs4-/-
SA072541MQ_23Ndufs4-/-
SA072542MQ_39WT
SA072543MQ_13WT
SA072544MQ_15WT
SA072545MQ_16WT
SA072546MQ_18WT
SA072547MQ_11WT
SA072548MQ_9WT
SA072549MQ_3WT
SA072550MQ_6WT
SA072551MQ_7WT
SA072552MQ_20WT
SA072553MQ_22WT
SA072554MQ_32WT
SA072555MQ_33WT
SA072556MQ_36WT
SA072557MQ_31WT
SA072558MQ_30WT
SA072559MQ_25WT
SA072560MQ_27WT
SA072561MQ_28WT
SA072562MQ_1WT
Showing results 1 to 40 of 40

Collection:

Collection ID:CO001114
Collection Summary:Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used.
Sample Type:Muscle

Treatment:

Treatment ID:TR001134
Treatment Summary:The animals did not receive any treatment.

Sample Preparation:

Sampleprep ID:SP001127
Sampleprep Summary:Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform).
Sampleprep Protocol Filename:LC_quadriceps_sample_prep_protocol.pdf

Chromatography:

Chromatography ID:CH001242
Instrument Name:Agilent 1200
Column Name:Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um)
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN001758
Analysis Type:MS
Chromatography ID:CH001242
Num Factors:2
Num Metabolites:44
Units:Normalized peak area
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