Summary of Study ST001117

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000748. The data can be accessed directly via it's Project DOI: 10.21228/M80D69 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001117
Study Title	A Metabolomic study of hibernating Syrian hamster brain: in search of neuroprotective agents
Study Type	Multiplatform non-targeted metabolomics
Study Summary	hamster brain samples, divided in 3 groups: torpor, arousal, control group were compared via metabolomics analysis
Institute	Universidad CEU San Pablo
Laboratory	CEMBIO (Centre for Metabolomics and Bioanalysis)
Last Name	Gonzalez-Riano
First Name	Carolina
Address	Facultad de Farmacia, Universidad CEU San Pablo, Campus Monteprincipe, Boadilla del Monte, Boadilla del Monte, Madrid, 28668, Spain
Email	car.gonzalez@ceindo.ceu.es
Phone	00 34 91 3724753
Submit Date	2018-12-21
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	GC-MS/LC-MS
Release Date	2019-01-22
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000748
Project DOI:	doi: 10.21228/M80D69
Project Title:	A Metabolomic study of hibernating Syrian hamster brain: in search of neuroprotective agents
Project Summary:	hamster brain samples, divided in 3 groups: torpor, arousal, control group were compared via metabolomics analysis
Institute:	Universidad CEU San Pablo
Last Name:	Gonzalez-Riano
First Name:	Carolina
Address:	Facultad de Farmacia, Universidad CEU San Pablo, Campus Monteprincipe, Boadilla del Monte, Boadilla del Monte, Madrid, 28668, Spain
Email:	car.gonzalez@ceindo.ceu.es
Phone:	00 34 91 3724753

Subject:

Subject ID:	SU001163
Subject Type:	Mammal
Subject Species:	Mesocricetus auratus
Taxonomy ID:	10036
Species Group:	Mammals

Factors:

Subject type: Mammal; Subject species: Mesocricetus auratus (Factor headings shown in green)

mb_sample_id	local_sample_id	hibernation
SA076217	A1	arousal
SA076218	A11	arousal
SA076219	A10	arousal
SA076220	A3	arousal
SA076221	A6	arousal
SA076222	C4	control
SA076223	C5	control
SA076224	C3	control
SA076225	C2	control
SA076226	C1	control
SA076227	T10	torpor
SA076228	T11	torpor
SA076229	T5	torpor
SA076230	T2	torpor
SA076231	T4	torpor

Showing results 1 to 15 of 15

Showing results 1 to 15 of 15

Collection:

Collection ID:	CO001157
Collection Summary:	A total of 15 male 3-month-old Syrian hamsters had free access to food and water and were kept at 23C with an 8:16-h light/dark cycle for a four to six-week acclimatization period in our animal facility. All animals were euthanized by decapitation. Brains were then removed and immediately transferred to a N2(l)-containing recipient to freeze the tissues.
Sample Type:	Brain

Treatment:

Treatment ID:	TR001177
Treatment Summary:	the whole right hemisphere (300 mg approx.) was analyzed to decrease possible biological variability due to the brain region employed. Brain homogenate was prepared by adding cold (-20°C) methanol:water (1:1, v/v), (1:10 tissue:solvent). Tissue disruption was achieved with TissueLyser LT homogenizer (Qiagen, Germany) for metabolite extraction. Subsequently, 100 μL of brain tissue homogenate was vortex-mixed with 320 μL of methanol for 2 min, followed by the addition of 80 μL of MTBE for the extraction of non-polar compounds. Then, vials were rapidly capped and placed on a shaker for 1 h at room temperature. The extracted samples were centrifuged at 4000 g for 20 min at 20°C. For GC-MS analysis, 300 μL of supernatant was evaporated to dryness (SpeedVac Concentrator System, Thermo Fisher Scientific, Waltham, MA, USA). Methoxymation was then performed with 20 µL O-methoxyamine hydrochloride (15 mg/mL in pyridine) and vigorously vortex-mixed for 5 min. Vials were then incubated in darkness at room temperature for 16 hours. For silylation, 20 μL of BSTFA:TMCS (99:1) was added, vortex-mixed for 5 min, and capped vials were placed in the oven at 70°C for 1 h. Finally, 100 μL of heptane containing C18:0 methyl ester (10 ppm) as Internal Standard (IS) was added to each vial prior to injection. For LC-MS analysis, 90 μL of supernatant was transferred to an Ultra-High Performance Liquid Chromatography-Mass (UHPLC-MS) chromatography vial with insert and was directly injected into the system. For CE-MS analysis, 200 μL of initial brain homogenate was centrifuged separately at 16000 g for 30 min at 15°C. 150 μL of supernatant was evaporated to dryness using the SpeedVac, and re-suspended in 150 μL of 0.2 mM Methionine Sulfone (IS) in 0.1 M formic acid. Samples were vortex-mixed, sonicated, and then centrifuged at 16000 g for 20 min at 4°C. Finally, 100 μL of supernatant was transferred to a CE-MS vial for the analysis.

Treatment ID:

TR001177

Treatment Summary:

the whole right hemisphere (300 mg approx.) was analyzed to decrease possible biological variability due to the brain region employed. Brain homogenate was prepared by adding cold (-20°C) methanol:water (1:1, v/v), (1:10 tissue:solvent). Tissue disruption was achieved with TissueLyser LT homogenizer (Qiagen, Germany) for metabolite extraction. Subsequently, 100 μL of brain tissue homogenate was vortex-mixed with 320 μL of methanol for 2 min, followed by the addition of 80 μL of MTBE for the extraction of non-polar compounds. Then, vials were rapidly capped and placed on a shaker for 1 h at room temperature. The extracted samples were centrifuged at 4000 g for 20 min at 20°C. For GC-MS analysis, 300 μL of supernatant was evaporated to dryness (SpeedVac Concentrator System, Thermo Fisher Scientific, Waltham, MA, USA). Methoxymation was then performed with 20 µL O-methoxyamine hydrochloride (15 mg/mL in pyridine) and vigorously vortex-mixed for 5 min. Vials were then incubated in darkness at room temperature for 16 hours. For silylation, 20 μL of BSTFA:TMCS (99:1) was added, vortex-mixed for 5 min, and capped vials were placed in the oven at 70°C for 1 h. Finally, 100 μL of heptane containing C18:0 methyl ester (10 ppm) as Internal Standard (IS) was added to each vial prior to injection. For LC-MS analysis, 90 μL of supernatant was transferred to an Ultra-High Performance Liquid Chromatography-Mass (UHPLC-MS) chromatography vial with insert and was directly injected into the system. For CE-MS analysis, 200 μL of initial brain homogenate was centrifuged separately at 16000 g for 30 min at 15°C. 150 μL of supernatant was evaporated to dryness using the SpeedVac, and re-suspended in 150 μL of 0.2 mM Methionine Sulfone (IS) in 0.1 M formic acid. Samples were vortex-mixed, sonicated, and then centrifuged at 16000 g for 20 min at 4°C. Finally, 100 μL of supernatant was transferred to a CE-MS vial for the analysis.

Sample Preparation:

Sampleprep ID:	SP001170
Sampleprep Summary:	the whole right hemisphere (300 mg approx.) was analyzed to decrease possible biological variability due to the brain region employed. Brain homogenate was prepared by adding cold (-20°C) methanol:water (1:1, v/v), (1:10 tissue:solvent). Tissue disruption was achieved with TissueLyser LT homogenizer (Qiagen, Germany) for metabolite extraction. Subsequently, 100 μL of brain tissue homogenate was vortex-mixed with 320 μL of methanol for 2 min, followed by the addition of 80 μL of MTBE for the extraction of non-polar compounds. Then, vials were rapidly capped and placed on a shaker for 1 h at room temperature. The extracted samples were centrifuged at 4000 g for 20 min at 20°C. For GC-MS analysis, 300 μL of supernatant was evaporated to dryness (SpeedVac Concentrator System, Thermo Fisher Scientific, Waltham, MA, USA). Methoxymation was then performed with 20 µL O-methoxyamine hydrochloride (15 mg/mL in pyridine) and vigorously vortex-mixed for 5 min. Vials were then incubated in darkness at room temperature for 16 hours. For silylation, 20 μL of BSTFA:TMCS (99:1) was added, vortex-mixed for 5 min, and capped vials were placed in the oven at 70°C for 1 h. Finally, 100 μL of heptane containing C18:0 methyl ester (10 ppm) as Internal Standard (IS) was added to each vial prior to injection. For LC-MS analysis, 90 μL of supernatant was transferred to an Ultra-High Performance Liquid Chromatography-Mass (UHPLC-MS) chromatography vial with insert and was directly injected into the system. For CE-MS analysis, 200 μL of initial brain homogenate was centrifuged separately at 16000 g for 30 min at 15°C. 150 μL of supernatant was evaporated to dryness using the SpeedVac, and re-suspended in 150 μL of 0.2 mM Methionine Sulfone (IS) in 0.1 M formic acid. Samples were vortex-mixed, sonicated, and then centrifuged at 16000 g for 20 min at 4°C. Finally, 100 μL of supernatant was transferred to a CE-MS vial for the analysis

Sampleprep ID:

SP001170

Sampleprep Summary:

Chromatography:

Chromatography ID:	CH001279
Instrument Name:	Agilent 7890A
Column Name:	Agilent 122-5532G
Chromatography Type:	GC

Chromatography ID:	CH001280
Instrument Name:	Agilent 1290 Infinity
Column Name:	Agilent Zorbax Eclipse Plus C8 (150 x 2.1mm, 1.8 um)
Chromatography Type:	Reversed phase

Chromatography ID:	CH001281
Instrument Name:	Agilent 7100 CE
Column Name:	Fused-silica capillary from Agilent Technologies
Chromatography Type:	CE

Chromatography ID:	CH001282
Instrument Name:	Agilent 1290 Infinity
Column Name:	Agilent Zorbax Eclipse Plus C8 (150 x 2.1mm, 1.8 um)
Chromatography Type:	Reversed phase

Analysis:

Analysis ID:	AN001813
Analysis Type:	MS
Chromatography ID:	CH001279
Num Factors:	3
Num Metabolites:	122
Units:	peak area

Analysis ID:	AN001814
Analysis Type:	MS
Chromatography ID:	CH001280
Num Factors:	3
Has Mz:	1
Has Rt:	1
Rt Units:	Minutes
Results File:	ST001117_AN001814_Results.txt
Units:	peak area

Analysis ID:	AN001815
Analysis Type:	MS
Chromatography ID:	CH001281
Num Factors:	3
Has Mz:	1
Has Rt:	1
Rt Units:	Minutes
Results File:	ST001117_AN001815_Results.txt
Units:	peak area

Analysis ID:	AN001816
Analysis Type:	MS
Chromatography ID:	CH001282
Num Factors:	3
Has Mz:	1
Has Rt:	1
Rt Units:	Minutes
Results File:	ST001117_AN001816_Results.txt
Units:	peak area