Summary of study ST001158

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000776. The data can be accessed directly via it's Project DOI: 10.21228/M8CH5C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001158
Study TitleThe gut microbiota plays a central role to modulate the plasma metabolome in response to chronic Angiotensin II infusion (part-II)
Study SummarySix week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. Our primary goal was to identify metabolites which were differentially regulated in conventional mice treated with AngII, but not in GF mice, indicating that these metabolites are microbial in origin. Following minipump implantation, animals were housed singly to prevent cross-contamination of microbiota. At the end of fourth week, feces and blood were collected. Both plasma and feces samples were processed and analyzed by using Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS/MS) for metabolite detection (Metabolon).
Institute
Johns Hopkins University
DepartmentDepartment of Physiology
LaboratoryPluznick Lab
Last NamePluznick
First NameJennifer
Address725 N. Wolfe St., WBSB 205, Baltimore, MD 21205
Emailjpluznick@jhmi.edu
Phone410 614 4660
Submit Date2019-03-11
Raw Data AvailableYes
Raw Data File Type(s).xlsx
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Jennifer Pluznick Jennifer Pluznick
https://dx.doi.org/10.21228/M8CH5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000776
Project DOI:doi: 10.21228/M8CH5C
Project Title:The gut microbiota plays a central role to modulate the plasma metabolome in response to chronic Angiotensin II infusion
Project Summary:Six week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. Our primary goal was to identify metabolites which were differentially regulated in conventional mice treated with AngII, but not in GF mice, indicating that these metabolites are microbial in origin. Following minipump implantation, animals were housed singly to prevent cross-contamination of microbiota. At the end of fourth week, feces and blood were collected. Both plasma and feces samples were processed and analyzed by using Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS/MS) for metabolite detection (Metabolon).
Institute:Johns Hopkins University
Last Name:Pluznick
First Name:Jennifer
Address:725 N. Wolfe St., WBSB 205, Baltimore, MD 21205
Email:jpluznick@jhmi.edu
Phone:410 614 4660

Subject:

Subject ID:SU001223
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male and female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample Treatment
SA080342JHOP-05731Feces Angiotensin II
SA080343JHOP-05730Feces Angiotensin II
SA080344JHOP-05732Feces Angiotensin II
SA080345JHOP-05740Feces Angiotensin II
SA080346JHOP-05739Feces Angiotensin II
SA080347JHOP-05729Feces Angiotensin II
SA080348JHOP-05728Feces Angiotensin II
SA080349JHOP-05744Feces Angiotensin II
SA080350JHOP-05743Feces Angiotensin II
SA080351JHOP-05742Feces Angiotensin II
SA080352JHOP-05727Feces Angiotensin II
SA080353JHOP-05741Feces Angiotensin II
SA080354JHOP-05738Feces Saline
SA080355JHOP-05737Feces Saline
SA080356JHOP-05733Feces Saline
SA080357JHOP-05724Feces Saline
SA080358JHOP-05723Feces Saline
SA080359JHOP-05722Feces Saline
SA080360JHOP-05725Feces Saline
SA080361JHOP-05726Feces Saline
SA080362JHOP-05735Feces Saline
SA080363JHOP-05734Feces Saline
SA080364JHOP-05721Feces Saline
SA080365JHOP-05736Feces Saline
Showing results 1 to 24 of 24

Collection:

Collection ID:CO001217
Collection Summary:Feces
Collection Protocol Filename:Sample_Collection-Feces_Pluznick.pdf
Sample Type:Feces
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001238
Treatment Summary:Six week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps.
Treatment Protocol Filename:Treatment_Protocol__Pluznick.pdf

Sample Preparation:

Sampleprep ID:SP001231
Sampleprep Summary:Samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.
Sampleprep Protocol Filename:Sample_Preparation_Pluznick.pdf

Combined analysis:

Analysis ID AN001916
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer
Column C18 column
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE
Units Peaks area

Chromatography:

Chromatography ID:CH001389
Methods Filename:Ultrahigh_Performance_LC_Method_Pluznick.pdf
Instrument Name:Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer
Column Name:C18 column
Chromatography Type:Reversed phase

MS:

MS ID:MS001772
Analysis ID:AN001916
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:None
Ion Mode:NEGATIVE
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