Summary of study ST001158

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000776. The data can be accessed directly via it's Project DOI: 10.21228/M8CH5C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001158
Study TitleThe gut microbiota plays a central role to modulate the plasma metabolome in response to chronic Angiotensin II infusion (part-II)
Study SummarySix week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. Our primary goal was to identify metabolites which were differentially regulated in conventional mice treated with AngII, but not in GF mice, indicating that these metabolites are microbial in origin. Following minipump implantation, animals were housed singly to prevent cross-contamination of microbiota. At the end of fourth week, feces and blood were collected. Both plasma and feces samples were processed and analyzed by using Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS/MS) for metabolite detection (Metabolon).
Institute
Johns Hopkins University
DepartmentDepartment of Physiology
LaboratoryPluznick Lab
Last NamePluznick
First NameJennifer
Address725 N. Wolfe St., WBSB 205, Baltimore, MD 21205
Emailjpluznick@jhmi.edu
Phone410 614 4660
Submit Date2019-03-11
Raw Data AvailableYes
Raw Data File Type(s).xlsx
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Jennifer Pluznick Jennifer Pluznick
https://dx.doi.org/10.21228/M8CH5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000776
Project DOI:doi: 10.21228/M8CH5C
Project Title:The gut microbiota plays a central role to modulate the plasma metabolome in response to chronic Angiotensin II infusion
Project Summary:Six week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. Our primary goal was to identify metabolites which were differentially regulated in conventional mice treated with AngII, but not in GF mice, indicating that these metabolites are microbial in origin. Following minipump implantation, animals were housed singly to prevent cross-contamination of microbiota. At the end of fourth week, feces and blood were collected. Both plasma and feces samples were processed and analyzed by using Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS/MS) for metabolite detection (Metabolon).
Institute:Johns Hopkins University
Last Name:Pluznick
First Name:Jennifer
Address:725 N. Wolfe St., WBSB 205, Baltimore, MD 21205
Email:jpluznick@jhmi.edu
Phone:410 614 4660
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