Summary of Study ST001184

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000797. The data can be accessed directly via it's Project DOI: 10.21228/M8NT2T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001184
Study TitleSulfonates in marine plankton
Study SummaryC2- and C3-sulfonates were quantified in marine plankton particulate samples.
Institute
University of Washington
DepartmentOceanography
LaboratoryIngalls
Last NameDurham
First NameBryndan
AddressBenjamin Hall Rm 330
Emailbpdurham@uw.edu
Phone206-685-4196
Submit Date2019-03-29
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2019-07-03
Release Version1
Bryndan Durham Bryndan Durham
https://dx.doi.org/10.21228/M8NT2T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000797
Project DOI:doi: 10.21228/M8NT2T
Project Title:Sulfonates in marine plankton
Project Summary:C2- and C3-sulfonates were quantified in marine plankton particulate samples.
Institute:University of Washington
Department:Oceanography
Laboratory:Ingalls
Last Name:Durham
First Name:Bryndan
Address:Benjamin Hall Rm 330
Email:bpdurham@uw.edu
Phone:206-685-4196
Funding Source:NSF and Simons Foundation
Publications:Durham et al., Nature Microbiology

Subject:

Subject ID:SU001252
Subject Type:Other
Subject Species:Marine Plankton
Species Group:Plankton

Factors:

Subject type: Other; Subject species: Marine Plankton (Factor headings shown in green)

mb_sample_id local_sample_id Replicate
SA082255160627_Smp_9R11
SA082256160627_Smp_6R11
SA082257160705_Smp_16R21
SA082258160705_Smp_17R11
SA082259160627_Smp_8R11
SA082260160705_Smp_15R11
SA082261160705_Smp_13R11
SA082262160627_Smp_3R11
SA082263160705_Smp_11R11
SA082264160705_Smp_12R11
SA082265160627_Smp_10R11
SA082266160705_Smp_18R11
SA082267160705_Smp_14R11
SA082268160627_Smp_2R11
SA082269160705_Smp_20R11
SA082270160627_Smp_4R11
SA082271160627_Smp_5R11
SA082272160705_Smp_19R11
SA082273160627_Smp_7R11
SA082274pooled sample1
SA082275160627_Smp_1R11
SA082276160705_Smp_14R22
SA082277160627_Smp_4R22
SA082278160705_Smp_13R22
SA082279160705_Smp_20R22
SA082280160705_Smp_12R22
SA082281160627_Smp_5R22
SA082282160627_Smp_6R22
SA082283160627_Smp_9R22
SA082284160627_Smp_10R22
SA082285160705_Smp_15R22
SA082286160627_Smp_8R22
SA082287160705_Smp_17R22
SA082288160627_Smp_3R22
SA082289160627_Smp_1R22
SA082290160711_Smp_11R22
SA082291160711_Smp_16R22
SA082292160627_Smp_7R22
SA082293160705_Smp_19R22
SA082294160627_Smp_2R22
SA082295160711_Smp_18R22
SA082296160627_Smp_6R33
SA082297160627_Smp_3R33
SA082298160627_Smp_7R33
SA082299160627_Smp_8R33
SA082300160627_Smp_10R33
SA082301160627_Smp_9R33
SA082302160711_Smp_14R33
SA082303160627_Smp_1R33
SA082304160705_Smp_17R33
SA082305160711_Smp_18R33
SA082306160627_Smp_4R33
SA082307160705_Smp_20R33
SA082308160705_Smp_19R33
SA082309160627_Smp_5R33
SA082310160705_Smp_16R33
SA082311160705_Smp_13R33
SA082312160627_Smp_2R33
SA082313160711_Smp_11R33
SA082314160705_Smp_15R33
SA082315160711_Smp_12R33
SA082316170117_Smp_Och_AA
SA082317161107_Smp_2090_AA
SA082318161107_Smp_SA30_AA
SA082319161107_Smp_SA42_AA
SA082320170117_Smp_SA48_AA
SA082321161116_Smp_SA16_AA
SA082322170117_Smp_SA11_AA
SA082323170117_Smp_DSS3_AA
SA082324161116_Smp_SA7_AA
SA082325161107_Smp_SA33_AA
SA082326170821_Smp_As9601_AA
SA082327161107_Smp_To_AA
SA082328161107_Smp_Tp_AA
SA082329170821_Smp_1771_AA
SA082330170821_Smp_1314_AA
SA082331170213_Smp_Pcx_AA
SA082332170213_Smp_Pt_AA
SA082333161107_Smp_371_AA
SA082334161107_Smp_Cy_AA
SA082335161107_Smp_Np_AA
SA082336161107_Smp_SA55_AA
SA082337170821_Smp_449_AA
SA082338161114_Smp_8102_AA
SA082339161116_Smp_SA60_AA
SA082340170117_Smp_SA36_AA
SA082341161116_Smp_SA59_AA
SA082342170821_Smp_2021_AA
SA082343170821_Smp_Nat_AA
SA082344180227_Smp_3430_AA
SA082345170117_Smp_SA53_AA
SA082346170821_Smp_1314P_AA
SA082347170821_Smp_1545_AA
SA082348161107_Smp_SA42_BB
SA082349170117_Smp_SA53_BB
SA082350170117_Smp_DSS3_BB
SA082351161107_Smp_371_BB
SA082352170821_Smp_As9601_BB
SA082353170821_Smp_1314P_BB
SA082354161107_Smp_Cy_BB
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Collection:

Collection ID:CO001246
Collection Summary:Cultured marine plankton cells grown to mid-to-late exponential phase were filtered onto 0.2-micron filters and extracted for metabolites as described in methods. Environmental plankton samples were collected onto 0.2-micron filters during a diel study (Diel_study_StationCastNumber) in the North Pacific.
Sample Type:Plankton cells

Treatment:

Treatment ID:TR001267
Treatment Summary:Marine plankton cells were cultured according to standard protocols, and environmental samples were collected in situ.

Sample Preparation:

Sampleprep ID:SP001260
Sampleprep Summary:Marine plankton cells were extracted using the described protocols.
Sampleprep Protocol Filename:SP_Ingalls_extraction_protocol.pdf

Chromatography:

Chromatography ID:CH001425
Methods Filename:CH_Ingalls_LC_protocol.pdf
Instrument Name:Waters Acquity I-Class
Column Name:SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
Column Temperature:30
Flow Rate:0.15 mL/min
Solvent A:85% acetonitrile/15% water; 10 mM ammonium carbonate
Solvent B:40% acetonitrile/60% water; 10 mM ammonium carbonate
Chromatography Type:HILIC

Analysis:

Analysis ID:AN001970
Analysis Type:MS
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
Chromatography ID:CH001425
Num Factors:9
Num Metabolites:5
Units:nanomolar in extraction vial
  
Analysis ID:AN001971
Analysis Type:MS
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
Chromatography ID:CH001425
Num Factors:9
Num Metabolites:5
Units:picomoles per liter of filtered seawater
  
Analysis ID:AN001972
Analysis Type:MS
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
Chromatography ID:CH001425
Num Factors:9
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST001184_AN001972_Results.txt
Units:peak area per liter of filtered seawater
  
Analysis ID:AN001973
Analysis Type:MS
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
Chromatography ID:CH001425
Num Factors:9
Has Mz:1
Has Rt:1
Rt Units:Seconds
Results File:ST001184_AN001973_Results.txt
Units:peak area
  
Analysis ID:AN001974
Analysis Type:MS
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
Chromatography ID:CH001425
Num Factors:9
Has Mz:1
Has Rt:1
Rt Units:Seconds
Results File:ST001184_AN001974_Results.txt
Units:Peak area
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