Summary of Study ST001191
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000803. The data can be accessed directly via it's Project DOI: 10.21228/M8WD6C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001191 |
| Study Title | Metabolome of ginsenoside anti-tumor |
| Study Type | Metabonomics |
| Study Summary | Metabonome profiling analysis reveals the protein-metabolite interaction network of ginsenoside anti-tumor |
| Institute | Nankai University |
| Department | State Key Laboratory of Medicinal Chemical Biology |
| Laboratory | Bai group |
| Last Name | Zhihua |
| First Name | Wang |
| Address | 38 Tongyan Road |
| 15822278821@163.com | |
| Phone | 02285358344 |
| Submit Date | 2019-06-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-07-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000803 |
| Project DOI: | doi: 10.21228/M8WD6C |
| Project Title: | Metabonome of ginsenoside |
| Project Type: | Metabonomics |
| Project Summary: | Metabonome profiling analysis reveals the protein-metabolite interaction network of ginsenoside anti-tumor |
| Institute: | Nankai University |
| Department: | State Key Laboratory of Medicinal Chemical Biology |
| Laboratory: | Bai group |
| Last Name: | Zhihua |
| First Name: | Wang |
| Address: | 38 Tongyan Road |
| Email: | 15822278821@163.com |
| Phone: | 02285358344 |
Subject:
| Subject ID: | SU001258 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA082709 | NEG C-1 | Control |
| SA082710 | NEG C-5 | Control |
| SA082711 | NEG C-6 | Control |
| SA082712 | NEG C-4 | Control |
| SA082713 | NEG C-3 | Control |
| SA082714 | NEG C-2 | Control |
| SA082715 | NEG QC-1 | QC |
| SA082716 | NEG QC-2 | QC |
| SA082717 | NEG QC-3 | QC |
| SA082718 | NEG S-6 | Sample |
| SA082719 | NEG S-2 | Sample |
| SA082720 | NEG S-1 | Sample |
| SA082721 | NEG S-3 | Sample |
| SA082722 | NEG S-4 | Sample |
| SA082723 | NEG S-5 | Sample |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO001252 |
| Collection Summary: | Total metabolites was extracted from the per group cell (~1x10e7) using 1 ml of methanolic acetonitrile aqueous solution (2:2:1, v/v), vortex for 60 s, hypothermia for 30 min, 2 times, place the protein for 1 h at -20 ° C, centrifuge at 14000 rcf for 20 min at 4 ° C, and freeze the supernatant. -80 ° C to save the sample |
| Sample Type: | Tumor cells |
Treatment:
| Treatment ID: | TR001273 |
| Treatment Summary: | After cells growth in 37°C for 12 h, 0.25mg/mL ginsenosides was fed of each well and continue cultured with for 12h |
Sample Preparation:
| Sampleprep ID: | SP001266 |
| Sampleprep Summary: | Total metabolites was extracted from the per group cell (~1x10e7) using 1 ml of methanolic acetonitrile aqueous solution (2:2:1, v/v), vortex for 60 s, hypothermia for 30 min, 2 times, place the protein for 1 h at -20 ° C, centrifuge at 14000 rcf for 20 min at 4 ° C, and freeze the supernatant. -80 ° C to save the sample |
Chromatography:
| Chromatography ID: | CH001431 |
| Instrument Name: | Agilent 1290 |
| Column Temperature: | 25 ° C |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 25 mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN001983 |
| Analysis Type: | MS |
| Chromatography ID: | CH001431 |
| Num Factors: | 3 |
| Num Metabolites: | 72 |
| Rt Units: | Seconds |
| Units: | intensity |
