Summary of study ST001191

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000803. The data can be accessed directly via it's Project DOI: 10.21228/M8WD6C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001191
Study TitleMetabolome of ginsenoside anti-tumor
Study TypeMetabonomics
Study SummaryMetabonome profiling analysis reveals the protein-metabolite interaction network of ginsenoside anti-tumor
Institute
Nankai University
DepartmentState Key Laboratory of Medicinal Chemical Biology
LaboratoryBai group
Last NameZhihua
First NameWang
Address38 Tongyan Road
Email15822278821@163.com
Phone02285358344
Submit Date2019-06-09
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Wang Zhihua Wang Zhihua
https://dx.doi.org/10.21228/M8WD6C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000803
Project DOI:doi: 10.21228/M8WD6C
Project Title:Metabonome of ginsenoside
Project Type:Metabonomics
Project Summary:Metabonome profiling analysis reveals the protein-metabolite interaction network of ginsenoside anti-tumor
Institute:Nankai University
Department:State Key Laboratory of Medicinal Chemical Biology
Laboratory:Bai group
Last Name:Zhihua
First Name:Wang
Address:38 Tongyan Road
Email:15822278821@163.com
Phone:02285358344

Subject:

Subject ID:SU001258
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA082709NEG C-1Control
SA082710NEG C-5Control
SA082711NEG C-6Control
SA082712NEG C-4Control
SA082713NEG C-3Control
SA082714NEG C-2Control
SA082715NEG QC-1QC
SA082716NEG QC-2QC
SA082717NEG QC-3QC
SA082718NEG S-6Sample
SA082719NEG S-2Sample
SA082720NEG S-1Sample
SA082721NEG S-3Sample
SA082722NEG S-4Sample
SA082723NEG S-5Sample
Showing results 1 to 15 of 15

Collection:

Collection ID:CO001252
Collection Summary:Total metabolites was extracted from the per group cell (~1x10e7) using 1 ml of methanolic acetonitrile aqueous solution (2:2:1, v/v), vortex for 60 s, hypothermia for 30 min, 2 times, place the protein for 1 h at -20 ° C, centrifuge at 14000 rcf for 20 min at 4 ° C, and freeze the supernatant. -80 ° C to save the sample
Sample Type:Tumor cells

Treatment:

Treatment ID:TR001273
Treatment Summary:After cells growth in 37°C for 12 h, 0.25mg/mL ginsenosides was fed of each well and continue cultured with for 12h

Sample Preparation:

Sampleprep ID:SP001266
Sampleprep Summary:Total metabolites was extracted from the per group cell (~1x10e7) using 1 ml of methanolic acetonitrile aqueous solution (2:2:1, v/v), vortex for 60 s, hypothermia for 30 min, 2 times, place the protein for 1 h at -20 ° C, centrifuge at 14000 rcf for 20 min at 4 ° C, and freeze the supernatant. -80 ° C to save the sample

Combined analysis:

Analysis ID AN001983
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system Agilent 1290
Column HILIC
MS Type ESI
MS instrument type QTOF
MS instrument name ABI Sciex 5600 TripleTOF
Ion Mode NEGATIVE
Units intensity

Chromatography:

Chromatography ID:CH001431
Instrument Name:Agilent 1290
Column Name:HILIC
Column Temperature:25 ° C
Flow Rate:0.3 mL/min
Solvent A:water + 25 mM ammonium acetate + 25 mM ammonia
Solvent B:Acetonitrile
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS001836
Analysis ID:AN001983
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The ESI source conditions after HILIC chromatographic separation are as follows: Ion Source Gas1 (Gas1): 60, Ion Source Gas 2 (Gas 2): 60, Curtain gas (CUR): 30, source temperature: 600 ° C, Ion Sapary Voltage Floating (ISVF) ± 5500 V (Positive and negative modes); TOF MS scan m/z range: 60-1000 Da, product ion scan m/z range: 25-1000 Da, TOF MS scan accumulation time 0.20 s/spectra, product ion scan accumulation time 0.05 s/spectra; secondary mass spectrometry is obtained using information dependent acquisition (IDA), and uses high sensitivity mode, Declustering potential (DP): ±60 V (both positive and negative modes), Collision Energy: 35±15 eV, IDA settings are as follows Exclude isotopes within 4 Da, Candidate ions to monitor per cycle: 6
Ion Mode:NEGATIVE
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