Summary of study ST001230

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000822. The data can be accessed directly via it's Project DOI: 10.21228/M8F69D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001230
Study TitleAquamin and Prevention of Colon Cancer (part-VI)
Study TypeMS analysis
Study SummaryWe propose to evaluate microbial and metabolic profiles in baseline and endpoint colonic mucosal, fecal, and serum samples from human patients at risk for CRC and enrolled in a 90-day phase I clinical trial. Patients will receive daily supplementation with calcium alone, a calcium-rich multimineral (Aquamin?), or placebo (maltodextrin) (n=10 per group). We hypothesize that dietary supplementation will correlate with CRC-protective metabolic profiles and that multimineral supplementation will generate more favorable profiles than calcium supplementation alone.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2019-07-23
Num Groups24
Total Subjects18
Study CommentsColorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer related death when both genders are combined. Epidemiologically, calcium intake has been protective against colonic adenomas and even colon cancer. Calcium supplementation has reduced the risk of colon adenoma formation in subjects with a history of previous colon polyps. The utility of calcium supplementation for colon cancer prevention is somewhat modulated by the modest or inconsistent level of protection afforded. Our preliminary data in mice and human enterocyte models shows that dietary supplementation with a multimineral supplement (Aquamin?) containing calcium in combination with 72 measureable trace minerals is more protective against tumors and epithelial growth dysregulation than calcium alone. One potential mechanism, supported by our rodent data, is that multimineral supplementation alters gut microbial populations to generate bile acid and short chain fatty acid (SCFA) profiles that are CRC-protective.
Raw Data AvailableYes
Raw Data File Type(s).m
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8F69D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000822
Project DOI:doi: 10.21228/M8F69D
Project Title:Aquamin and Prevention of Colon Cancer
Project Type:MS analysis
Project Summary:Effects of calcium and multi-mineral supplementation on gut microbes and microbially-derived metabolites in patients at risk for colon cancer
Institute:University of Michigan
Department:Pathology
Laboratory:Aslam Lab
Last Name:Aslam
First Name:Muhammad Nadeem
Address:Ann Arbor, MI
Email:mnaslam@umich.edu
Phone:734-936-1897

Subject:

Subject ID:SU001297
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time Point
SA087113S00031777Baseline
SA087114S00031775Baseline
SA087115S00031774Baseline
SA087116S00031778Baseline
SA087117S00031779Baseline
SA087118S00031763Baseline
SA087119S00031780Baseline
SA087120S00031773Baseline
SA087121S00031776Baseline
SA087122S00031767Baseline
SA087123S00031766Baseline
SA087124S00031772Baseline
SA087125S00031764Baseline
SA087126S00031768Baseline
SA087127S00031765Baseline
SA087128S00031769Baseline
SA087129S00031770Baseline
SA087130S00031771Baseline
SA087131S00031794Day 90
SA087132S00031792Day 90
SA087133S00031793Day 90
SA087134S00031795Day 90
SA087135S00031797Day 90
SA087136S00031791Day 90
SA087137S00031798Day 90
SA087138S00031796Day 90
SA087139S00031781Day 90
SA087140S00031784Day 90
SA087141S00031783Day 90
SA087142S00031782Day 90
SA087143S00031785Day 90
SA087144S00031786Day 90
SA087145S00031789Day 90
SA087146S00031788Day 90
SA087147S00031787Day 90
SA087148S00031790Day 90
Showing results 1 to 36 of 36

Collection:

Collection ID:CO001291
Collection Summary:NA
Sample Type:Feces

Treatment:

Treatment ID:TR001312
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP001305
Sampleprep Summary:Two-step solvent extraction. Supernatants are combined, dried, and re-suspended for LCMS separation by RPLC and measurements by ESI- QQQ MRM methods. Analytes are reported as uM, and CV's are generally <15%.
Sampleprep Protocol Filename:Bile_acids_analysis.doc

Combined analysis:

Analysis ID AN002043
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters Acquity BEH C18 (50 x 2mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units pmol/mg

Chromatography:

Chromatography ID:CH001483
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-I.m.zip
Instrument Name:Agilent 1260
Column Name:Waters Acquity BEH C18 (50 x 2mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001895
Analysis ID:AN002043
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NA
Ion Mode:POSITIVE
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