Summary of study ST001250

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000837. The data can be accessed directly via it's Project DOI: 10.21228/M8H118 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001250
Study TitleLuminal metabolome profiles of mice developed dextran sulfate sodium (DSS) colitis
Study SummaryLuminal (cecal) metabolome of DSS-colitis mice and control mice were compared.
Institute
University of Michigan
DepartmentInternal Medicine
Last NameKamada
First NameNobuhiko
Address1150 W Medical center Dr, Ann Arbor, MI 48109, USA
Emailnkamada@umich.edu
Phone+1-734-763-2142
Submit Date2019-09-12
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2019-10-11
Release Version1
Nobuhiko Kamada Nobuhiko Kamada
https://dx.doi.org/10.21228/M8H118
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000837
Project DOI:doi: 10.21228/M8H118
Project Title:Luminal metabolome profiles of mice developed dextran sulfate sodium (DSS) colitis
Project Summary:Intestinal inflammation alters the composition of the gut microbiota thereby changing luminal metabolite profile. To examine the changes in the luminal metabolites during intestinal inflammation, specific pathogen-free mice were given dextran sulfate sodium (DSS) to induce colitis. Cecal contents from DSS-induced colitis mice and control mice were harvested and metabolite profiles of them were analyzed.
Institute:University of Michigan
Last Name:Kamada
First Name:Nobuhiko
Address:1150 W Medical center Dr, Ann Arbor, MI 48109, USA
Email:nkamada@umich.edu
Phone:+1-734-763-2142

Subject:

Subject ID:SU001318
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male and female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA091006101901Control
SA091007101905Control
SA091008101904Control
SA091009101902Control
SA091010101903Control
SA091011101910DSS
SA091012101909DSS
SA091013101906DSS
SA091014101907DSS
SA091015101908DSS
Showing results 1 to 10 of 10

Collection:

Collection ID:CO001312
Collection Summary:Cecal contents of DSS-treated and control mice were harvested.
Sample Type:Feces

Treatment:

Treatment ID:TR001333
Treatment Summary:SPF C57BL/6 mice were originally purchased from Jackson Laboratories. Mice were treated with 1.5% DSS for 5 days.

Sample Preparation:

Sampleprep ID:SP001326
Sampleprep Summary:Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol.

Combined analysis:

Analysis ID AN002075 AN002076
Analysis type MS MS
Chromatography type CE CE
Chromatography system Agilent CE Agilent CE
Column Fused-silica, i.d. 50 µm(cation) COSMO(+), i.d.50 μm(anion)
MS Type ESI ESI
MS instrument type Other Other
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS
Ion Mode POSITIVE NEGATIVE
Units nmol/g nmol/g

Chromatography:

Chromatography ID:CH001512
Instrument Name:Agilent CE
Column Name:Fused-silica, i.d. 50 µm(cation)
Internal Standard:20 μM of methionine sulfone
Chromatography Type:CE
  
Chromatography ID:CH001513
Instrument Name:Agilent CE
Column Name:COSMO(+), i.d.50 μm(anion)
Internal Standard:20 μM of D-camphor-10-sulfonic acid (CSA)
Chromatography Type:CE

MS:

MS ID:MS001926
Analysis ID:AN002075
Instrument Name:Agilent CE-TOFMS
Instrument Type:Other
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:POSITIVE
  
MS ID:MS001927
Analysis ID:AN002076
Instrument Name:Agilent CE-TOFMS
Instrument Type:Other
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:NEGATIVE
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