Summary of study ST001295

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000876. The data can be accessed directly via it's Project DOI: 10.21228/M8FX1M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001295
Study TitleEstimating Platelet Mitochondrial Function in Patients with Sepsis - WB NMRs (part-II)
Study Typesingle timepoint
Study SummaryRelationships between platelet mitochondrial oxygen consumption rates (mOCR) and metabolites in platelets as measured by quantitative 1H-NMR metabolomics in WB. Comparison of mitochondrial function and metabolomics in patients with sepsis and non-sepsis ED patients
Institute
University of Michigan; University of Mississippi; University of Minnesota
DepartmentClinical Pharmacy (UMich); Emergency Medicine (UMiss)
LaboratoryStringer NMR Metabolomics Laboratory
Last NameMcHugh
First NameCora
Address428 Church St, Ann Arbor, MI, 48103, USA
EmailNMRmetabolomics@umich.edu
Phone7343530164
Submit Date2019-12-13
Num Groups2
Total Subjects26
Num Males14
Num Females12
Raw Data AvailableYes
Raw Data File Type(s).par, pdata, info, .xml
Analysis Type DetailNMR
Release Date2020-05-26
Release Version1
Cora McHugh Cora McHugh
https://dx.doi.org/10.21228/M8FX1M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000876
Project DOI:doi: 10.21228/M8FX1M
Project Title:Estimating Platelet Mitochondrial Function in Patients with Sepsis
Project Type:Quantitative NMR Metabolomics
Project Summary:Sepsis patients and ill controls admitted to the ED had matched whole blood (WB) and isolated platelet samples collected. The control group was chosen to represent an acutely ill 127cohort of the same racial, sex, and age as the sepsis cohort. NMR was run on extracted WB and platelets to determine how the metabolome reflects mitochondrial function
Institute:University of Michigan; University of Mississippi; University of Minnesota
Department:Clinical Pharmacy (UMich); Emergency Medicine (UMiss)
Laboratory:Stringer NMR Metabolomics Laboratory
Last Name:McHugh
First Name:Cora
Address:428 Church St, Ann Arbor, MI, 48103, USA
Email:NMRmetabolomics@umich.edu
Phone:7343530164
Funding Source:GM111400, K23GM113041
Contributors:Kathleen Stringer, Mike Puskarich, Marc McCann

Subject:

Subject ID:SU001369
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:32-67
Gender:Male and female
Human Race:Caucasian (C) and African American (AA)
Human Ethnicity:non-hispanic
Human Inclusion Criteria:Sepsis: 1) Suspected or confirmed infection; 2) Any two of four criteria of systemic inflammatory response in ED(18); 3) Age ≥ 18; 4) Lactate ≥ 2.0 mmol/L; 5) Enrollment within 2 hours of initiation of quantitative resuscitation protocol. Controls: admitted to the emergency department and had no medical conditions that required chronic administration of medication expected to affect platelet function (aspirin, PGY12 inhibitors, etc)
Human Exclusion Criteria:Exclusion criteria were:1) any primary diagnosis other than sepsis; 2) established Do Not Resuscitate status; 3) transferred from another hospital with sepsis therapy already initiated; 4) cardiopulmonary resuscitation (chest compression or defibrillation) prior to enrollment; 5) patient or legal representative unable to understand and sign informed consent.

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Condition
SA094121233_69Control
SA094122233_67Control
SA094123233_62Control
SA094124233_70Control
SA094125233_76Control
SA094126233_80Control
SA094127233_78Control
SA094128233_42Control
SA094129233_71Control
SA094130233_61Control
SA094131233_47Control
SA094132233_51Control
SA094133233_52Control
SA094134233_54Control
SA094135233_74Sepsis
SA094136233_43Sepsis
SA094137233_72Sepsis
SA094138233_48Sepsis
SA094139233_75Sepsis
SA094140233_44Sepsis
SA094141233_77Sepsis
SA094142233_49Sepsis
SA094143233_79Sepsis
SA094144233_46Sepsis
SA094145233_68Sepsis
SA094146233_59Sepsis
SA094147233_58Sepsis
SA094148233_57Sepsis
SA094149233_56Sepsis
SA094150233_60Sepsis
SA094151233_63Sepsis
SA094152233_55Sepsis
SA094153233_66Sepsis
SA094154233_64Sepsis
SA094155233_50Sepsis
Showing results 1 to 35 of 35

Collection:

Collection ID:CO001364
Collection Summary:WB samples (12 mL each) were collected by direct venipuncture or from an indwelling line into K2 EDTA-containing tubes. Samples were flash frozen and stored at -80C.
Sample Type:Blood (whole)
Collection Method:direct venipuncture or indwelling line
Collection Frequency:once
Volumeoramount Collected:12mL
Storage Conditions:-80℃
Collection Vials:K2 EDTA-containing Vacutainer tubes (BD 134367863; Becton-Dickinson, Franklin Lakes, NJ USA)
Additives:K2 EDTA

Treatment:

Treatment ID:TR001384
Treatment Summary:No treatment. Sepsis and non-sepsis controls had platelets isolated. Mitochondrial function was measured via mitochondrial oxygen consumption (mOCR)

Sample Preparation:

Sampleprep ID:SP001377
Sampleprep Summary:Methanol:cholorform precipitation
Sampleprep Protocol ID:WB methanol Chlorform precipitation
Sampleprep Protocol Filename:233.5.1_-_HAPS_WB_MeOHCHCl3_Precipitation
Processing Method:precipitation
Processing Storage Conditions:On ice
Extraction Method:Methanol chloroform precipitation
Extract Enrichment:Lyophilization
Extract Storage:-80℃
Sample Resuspension:500uL 50mM sodium phosphate buffer in D2O
Sample Spiking:4.99 mM DSS internal standard

Analysis:

Analysis ID:AN002156
Laboratory Name:University of Michigan BioNMR core
Analysis Type:NMR
Acquisition Date:04/05/2018, 04/09/2018, 04/10/2018
Software Version:TopSpin 4.0.7
Operator Name:Larisa Yeomans, Jae Hyun Kim
Num Factors:2
Num Metabolites:31
Units:µM

NMR:

NMR ID:NM000158
Analysis ID:AN002156
Instrument Name:Bruker 18.8 Tesla (800 MHz) NMR spectrometer ascend
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
NMR Comments:Arrayed for water saturation frequency and 90deg. pulse width for each sample
Standard Concentration:10% (~0.5mM)
Spectrometer Frequency:800 MHz
NMR Probe:Triple resonance inverse detection TCI cryoprobe
NMR Solvent:D20
NMR Tube Size:5mm
Shimming Method:Auto shim (gradient shimming)
Pulse Sequence:noesygppr1d
Water Suppression:saturation at 80 Hz induced field strength
Pulse Width:5.5ms
Offset Frequency:around -178Hz
Presaturation Power Level:80db
Chemical Shift Ref Cpd:DSS-d6
Temperature:25
Number Of Scans:32
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