Summary of study ST001318

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000895. The data can be accessed directly via it's Project DOI: 10.21228/M80Q38 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001318
Study TitleAtg32-mediated mitophagy promotes heat stress tolerance in Saccharomyces cerevisiae by sustaining spermidine and nitric oxide levels
Study Typemetabolomic identification
Study SummaryLooking for mitochondrial metabolites.
Institute
University of California, Davis
Last NamePaglia
First NameKelly
Address451 Health Sciences Drive, Room 1313, Davis, CA, 95616, USA
Emailkpaglia@ucdavis.edu
Phone5307528129
Submit Date2020-02-06
Study CommentsWest Coast Metabolomics Center - UC Davis
Raw Data AvailableYes
Raw Data File Type(s).cdf
Analysis Type DetailGC-MS
Release Date2020-07-08
Release Version1
Kelly Paglia Kelly Paglia
https://dx.doi.org/10.21228/M80Q38
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000895
Project DOI:doi: 10.21228/M80Q38
Project Title:Atg32-mediated mitophagy promotes heat stress tolerance in Saccharomyces cerevisiae by sustaining spermidine and nitric oxide levels
Project Type:metabolomics
Project Summary:Looking for mitochondrial metabolites.
Institute:University of California, Davis
Last Name:Paglia
First Name:Kelly
Address:451 Health Sciences Drive, Room 1313, Davis, CA, 95616, USA
Email:kpaglia@ucdavis.edu
Phone:5307528129

Subject:

Subject ID:SU001392
Subject Type:Other
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932

Factors:

Subject type: Other; Subject species: Saccharomyces cerevisiae (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA095232WT_30 Rep 4_0041 - WT_30
SA095233WT_30 Rep 5_0051 - WT_30
SA095234WT_30 Rep 1_0011 - WT_30
SA095235WT_30 Rep 3_0031 - WT_30
SA095236WT_30 Rep 2_0021 - WT_30
SA095237datg3_30 Rep 3_0082 - datg3_30
SA095238datg3_30 Rep 2_0072 - datg3_30
SA095239datg3_30 Rep 5_0102 - datg3_30
SA095240datg3_30 Rep 1_0062 - datg3_30
SA095241datg3_30 Rep 4_0092 - datg3_30
SA095242datg32_30 Rep 2_0123 - datg32_30
SA095243datg32_30 Rep 3_0133 - datg32_30
SA095244datg32_30 Rep 1_0113 - datg32_30
SA095245datg32_30 Rep 4_0143 - datg32_30
SA095246datg32_30 Rep 5_0153 - datg32_30
SA095247WT_37 Rep 2_0174 - WT_37
SA095248WT_37 Rep 5_0204 - WT_37
SA095249WT_37 Rep 1_0164 - WT_37
SA095250WT_37 Rep 4_0194 - WT_37
SA095251WT_37 Rep 3_0184 - WT_37
SA095252datg3_37 Rep 4_0245 - datg3_37
SA095253datg3_37 Rep 3_0235 - datg3_37
SA095254datg3_37 Rep 5_0255 - datg3_37
SA095255datg3_37 Rep 1_0215 - datg3_37
SA095256datg3_37 Rep 2_0225 - datg3_37
SA095257datg32_37 Rep 4_0296 - datg32_37
SA095258datg32_37 Rep 5_0306 - datg32_37
SA095259datg32_37 Rep 2_0276 - datg32_37
SA095260datg32_37 Rep 3_0286 - datg32_37
SA095261datg32_37 Rep 1_0266 - datg32_37
Showing results 1 to 30 of 30

Collection:

Collection ID:CO001387
Collection Summary:Yeast cells (WT and mutants of datg3 and datg32 were grown at 30 or 37 degrees in YPGE media to logarithmic phase, quenched with -20 chilled metanol:water (3:2), spun at 1000g for 5 minutes and the supernatant was discarded. Pellets were then saved at -80.
Sample Type:Yeast cells

Treatment:

Treatment ID:TR001407
Treatment Summary:Three genotypes- WT, atg3 null and atg32 null cells were grown at 30 or 37 degree for each group. (Total 6 samples per treatment).

Sample Preparation:

Sampleprep ID:SP001400
Sampleprep Summary:Added 500 uL 3:3:2 and vortex before transferring to 2 mL eppendorf tube. Added another 500 uL 3:3:2 to sample tubes and vortexed before transferring to 2 mL eppendorf tube.

Combined analysis:

Analysis ID AN002194
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus III GC TOF
Ion Mode POSITIVE
Units normalized peak height

Chromatography:

Chromatography ID:CH001609
Instrument Name:Agilent 6890N
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS002041
Analysis ID:AN002194
Instrument Name:Leco Pegasus III GC TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:Raw files are processed directly after data acquisition and stored as ChromaTOF-specific.
Ion Mode:POSITIVE
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