Summary of Study ST001321

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000898. The data can be accessed directly via it's Project DOI: 10.21228/M8MD6W This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001321
Study Title	Metabolomics in Escherichia coli mutant strains
Study Summary	Escherichia coli mutant strains were prepared for metabolomics analysis.
Institute	Manchester Institute of Biotechnology, University of Manchester
Department	Faculty of Science, University of Cadiz
Last Name	Valle
First Name	Antonio
Address	Avda. Republica Saharaui s/n
Email	antonio.valle@uca.es
Phone	0034 686588926
Submit Date	2019-07-09
Analysis Type Detail	LC-MS
Release Date	2020-03-02
Release Version	1

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Project:

Project ID:	PR000898
Project DOI:	doi: 10.21228/M8MD6W
Project Title:	Escherichia coli for succinic acid
Project Summary:	Succinic acid is a high-value product with industrial applications. This compound is produced by the bacteria Escherichia coli and its production has been increased by metabolic engineering strategies. In this work, metabolomics is used to analyze the effect of blocking of two competitive pathways of succinic acid to design new metabolic engineering strategies to improve succinic acid production.
Institute:	Manchester Institute of Biotechnology, University of Manchester;University of Cadiz
Department:	Faculty of Science, University of Cadiz
Last Name:	Valle
First Name:	Antonio
Address:	Avda. Republica Saharaui s/n
Email:	antonio.valle@uca.es
Phone:	0034 956 012820

Subject:

Subject ID:	SU001395
Subject Type:	Bacteria
Subject Species:	Escherichia coli
Taxonomy ID:	562
Species Group:	Bacteria

Factors:

Subject type: Bacteria; Subject species: Escherichia coli (Factor headings shown in green)

mb_sample_id	local_sample_id	Time
SA095502	171213_QC08	-
SA095503	171213_QC07	-
SA095504	171212_Blank02	-
SA095505	171212_QC06	-
SA095506	171212_QC10	-
SA095507	171212_QC07	-
SA095508	171213_QC05	-
SA095509	171213_QC04	-
SA095510	171213_QC03	-
SA095511	171213_QC02	-
SA095512	171212_QC08	-
SA095513	171213_QC01	-
SA095514	171213_QC06	-
SA095515	171212_QC05	-
SA095516	171212_QC02	-
SA095517	171213_Blank01	-
SA095518	171213_Blank02	-
SA095519	171213_QC11	-
SA095520	171212_QC01	-
SA095521	171212_Blank01	-
SA095522	171212_QC03	-
SA095523	171212_QC04	-
SA095524	171213_QC09	-
SA095525	171212_QC09	-
SA095526	171213_QC10	-
SA095527	171212_QC11	-
SA095528	171213_31	24 h
SA095529	171213_29	24 h
SA095530	171213_46	24 h
SA095531	171213_51	24 h
SA095532	171213_52	24 h
SA095533	171213_54	24 h
SA095534	171213_48	24 h
SA095535	171213_47	24 h
SA095536	171213_36	24 h
SA095537	171213_39	24 h
SA095538	171213_35	24 h
SA095539	171213_28	24 h
SA095540	171212_17	24 h
SA095541	171212_18	24 h
SA095542	171212_20	24 h
SA095543	171212_11	24 h
SA095544	171212_12	24 h
SA095545	171212_14	24 h
SA095546	171212_27	24 h
SA095547	171212_07	24 h
SA095548	171212_09	24 h
SA095549	171212_03	24 h
SA095550	171212_23	24 h
SA095551	171212_05	24 h
SA095552	171212_06	24 h
SA095553	171212_24	24 h
SA095554	171212_02	24 h
SA095555	171212_04	48 h
SA095556	171212_13	48 h
SA095557	171213_45	48 h
SA095558	171212_10	48 h
SA095559	171212_08	48 h
SA095560	171213_44	48 h
SA095561	171213_50	48 h
SA095562	171213_49	48 h
SA095563	171213_53	48 h
SA095564	171213_41	48 h
SA095565	171213_32	48 h
SA095566	171213_33	48 h
SA095567	171212_22	48 h
SA095568	171213_30	48 h
SA095569	171212_25	48 h
SA095570	171212_01	48 h
SA095571	171212_26	48 h
SA095572	171213_34	48 h
SA095573	171212_21	48 h
SA095574	171213_40	48 h
SA095575	171213_42	48 h
SA095576	171212_15	48 h
SA095577	171212_16	48 h
SA095578	171213_38	48 h
SA095579	171212_19	48 h
SA095580	171213_37	48 h
SA095581	171213_43	48 h

Showing results 1 to 80 of 80

Showing results 1 to 80 of 80

Collection:

Collection ID:	CO001390
Collection Summary:	Biomass samples were withdrawn for quenching with 60% methanol.
Sample Type:	Bacterial cells

Treatment:

Treatment ID:	TR001410
Treatment Summary:	The GC/MS data were firstly converted to mzXML format and imported into R. The data were then deconvolved by using eRah package. A total number of 612 features were detected and after removing features with over 20% RSD in the QCs.

Sample Preparation:

Sampleprep ID:	SP001403
Sampleprep Summary:	Metabolome were extracted from biomass snap-freezing in liquid nitrogen with 80% methanol and centrifuge at 15,000 x g -9, three times. The supernatant was dried with speed vacuum for 12 h and subsequently the samples were derivatized with methoxyamination followed by trimethylsylylation.

Chromatography:

Chromatography ID:	CH001611
Instrument Name:	Agilent 7200
Column Name:	Agilent VF-5ms (30 m x 0.25 mm, 0.25 µm)

Analysis:

Analysis ID:	AN002197
Laboratory Name:	SYNBIOCHEM (MIB, U. of Manchester)
Analysis Type:	MS
Chromatography ID:	CH001611
Num Factors:	3
Num Metabolites:	114
Units:	IU