Summary of study ST001349

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000919. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ3B This work is supported by NIH grant, U2C- DK119886.

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Study IDST001349
Study TitleMultiparous and Primiparous Simmental Dairy Cows
Study SummaryTargeted ESI-LC-MS/MS-based metabolomics was used to characterize metabolic alterations in the serum of cows, according to parities and feeding phase.
Institute
Institute of Animal Nutrition and Functional Plant Compounds
DepartmentDepartment for Farm Animals and Veterinary Public Health
LaboratoryChristian Doppler Laboratory for Innovative Gut Health Concepts of Livestock
Last NamePacífico
First NameCátia
AddressVeterinaerplatz 1
Emailcatia.pacifico@vetmeduni.ac.at
Phone06608389796
Submit Date2020-03-16
Total Subjects24
Num Males-
Num Females24
Analysis Type DetailLC-MS
Release Date2020-04-30
Release Version1
Cátia Pacífico Cátia Pacífico
https://dx.doi.org/10.21228/M8WQ3B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000919
Project DOI:doi: 10.21228/M8WQ3B
Project Title:Serum Metabolome of Simmental Dairy Cows Switched to High-Grain Diet during Early Lactation
Project Summary:In dairy cows, early lactation is associated with an energy deficit that is counteracted by increasing the amounts of grains in the diet. Yet, such diet often increases the odds for cows to develop digestive and systemic metabolic derailments, which can likely be detected as undesirable shifts in the serum metabolome. The present study aimed to identify the metabolic signatures of the serum metabolome of early lactation dairy cows fed high-grain diet.
Institute:Institute of Animal Nutrition and Functional Plant Compounds
Department:Department for Farm Animals and Veterinary Public Health
Laboratory:Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock
Last Name:Pacífico
First Name:Cátia
Address:Veterinaerplatz 1
Email:catia.pacifico@vetmeduni.ac.at
Phone:+436608389796

Subject:

Subject ID:SU001423
Subject Type:Mammal
Subject Species:Bos taurus
Taxonomy ID:9913
Gender:Female

Factors:

Subject type: Mammal; Subject species: Bos taurus (Factor headings shown in green)

mb_sample_id local_sample_id Day Group Parity
SA097900cow 15_Day 1414 control multiparous
SA097901cow 3_Day 1414 control multiparous
SA097902cow 5_Day 1414 control multiparous
SA097903cow 13_Day 1414 control multiparous
SA097904cow 4_Day 1414 control multiparous
SA097905cow 22_Day 1414 control multiparous
SA097906cow 1_Day 1414 control multiparous
SA097907cow 12_Day 1414 control multiparous
SA097908cow 2_Day 1414 control primiparous
SA097909cow 21_Day 1414 control primiparous
SA097910cow 14_Day 1414 control primiparous
SA097911cow 11_Day 1414 control primiparous
SA097912cow 6_Day 1414 treatment multiparous
SA097913cow 24_Day 1414 treatment multiparous
SA097914cow 19_Day 1414 treatment multiparous
SA097915cow 10_Day 1414 treatment multiparous
SA097916cow 20_Day 1414 treatment multiparous
SA097917cow 9_Day 1414 treatment multiparous
SA097918cow 7_Day 1414 treatment multiparous
SA097919cow 18_Day 1414 treatment multiparous
SA097920cow 8_Day 1414 treatment primiparous
SA097921cow 16_Day 1414 treatment primiparous
SA097922cow 23_Day 1414 treatment primiparous
SA097923cow 17_Day 1414 treatment primiparous
SA097924cow 5_Day 2121 control multiparous
SA097925cow 22_Day 2121 control multiparous
SA097926cow 15_Day 2121 control multiparous
SA097927cow 13_Day 2121 control multiparous
SA097928cow 12_Day 2121 control multiparous
SA097929cow 1_Day 2121 control multiparous
SA097930cow 4_Day 2121 control multiparous
SA097931cow 3_Day 2121 control multiparous
SA097932cow 11_Day 2121 control primiparous
SA097933cow 14_Day 2121 control primiparous
SA097934cow 2_Day 2121 control primiparous
SA097935cow 21_Day 2121 control primiparous
SA097936cow 24_Day 2121 treatment multiparous
SA097937cow 6_Day 2121 treatment multiparous
SA097938cow 9_Day 2121 treatment multiparous
SA097939cow 7_Day 2121 treatment multiparous
SA097940cow 20_Day 2121 treatment multiparous
SA097941cow 19_Day 2121 treatment multiparous
SA097942cow 10_Day 2121 treatment multiparous
SA097943cow 18_Day 2121 treatment multiparous
SA097944cow 17_Day 2121 treatment primiparous
SA097945cow 8_Day 2121 treatment primiparous
SA097946cow 16_Day 2121 treatment primiparous
SA097947cow 23_Day 2121 treatment primiparous
SA097948cow 4_Day 4242 control multiparous
SA097949cow 3_Day 4242 control multiparous
SA097950cow 22_Day 4242 control multiparous
SA097951cow 13_Day 4242 control multiparous
SA097952cow 5_Day 4242 control multiparous
SA097953cow 1_Day 4242 control multiparous
SA097954cow 12_Day 4242 control multiparous
SA097955cow 15_Day 4242 control multiparous
SA097956cow 14_Day 4242 control primiparous
SA097957cow 11_Day 4242 control primiparous
SA097958cow 2_Day 4242 control primiparous
SA097959cow 21_Day 4242 control primiparous
SA097960cow 10_Day 4242 treatment multiparous
SA097961cow 19_Day 4242 treatment multiparous
SA097962cow 20_Day 4242 treatment multiparous
SA097963cow 18_Day 4242 treatment multiparous
SA097964cow 24_Day 4242 treatment multiparous
SA097965cow 6_Day 4242 treatment multiparous
SA097966cow 7_Day 4242 treatment multiparous
SA097967cow 9_Day 4242 treatment multiparous
SA097968cow 17_Day 4242 treatment primiparous
SA097969cow 16_Day 4242 treatment primiparous
SA097970cow 8_Day 4242 treatment primiparous
SA097971cow 23_Day 4242 treatment primiparous
SA097972cow 14_Day 77 control primiparous
SA097973cow 11_Day 77 control primiparous
SA097974cow 21_Day 77 control primiparous
SA097975cow 2_Day 77 control primiparous
SA097976cow 16_Day 77 treatment primiparous
SA097977cow 17_Day 77 treatment primiparous
SA097978cow 23_Day 77 treatment primiparous
SA097979cow 8_Day 77 treatment primiparous
Showing results 1 to 80 of 80

Collection:

Collection ID:CO001418
Collection Summary:Blood samples were collected before the morning feeding from the jugular vein of all animals using vacutainer tubes. Samples were allowed to clot at 25°C for 2 h and all tubes were then centrifuged at 2000 × g at 4°C for 15 min to separate serum. Serum was pipetted into 2 mL tubes and stored at -80°C for further analysis.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001438
Treatment Summary:The feed contained either no additive as a control (CON) or a mineral-based endotoxin binder (EB).

Sample Preparation:

Sampleprep ID:SP001431
Sampleprep Summary:Determination of the serum metabolome was carried out using a targeted metabolomics approach based on the Biocrates MxP® Quant 500 kit. Ten μL aliquots of the serum samples were processed according to the manufacturer’s instructions.

Combined analysis:

Analysis ID AN002243 AN002244 AN002245
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase None (Direct infusion)
Chromatography system Agilent 1290 Agilent 1290 Agilent 1290
Column - - -
MS Type ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name Sciex 6500+ QTrap Sciex 6500+ QTrap Sciex 6500+ QTrap
Ion Mode POSITIVE NEGATIVE POSITIVE
Units µM µM µM

Chromatography:

Chromatography ID:CH001649
Chromatography Summary:RP-LC-MS/MS
Instrument Name:Agilent 1290
Column Name:-
Column Temperature:50 °C
Solvent A:1 L of water + 2 mL of formic acid
Solvent B:1 L of acetonitrile + 2 ml of formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH001650
Chromatography Summary:FIA-MS/MS
Instrument Name:Agilent 1290
Column Name:-
Column Temperature:25 °C
Solvent A:none
Solvent B:290 mL of methanol plus 10 mL of Biocrates' FIA solvent ampule
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS002089
Analysis ID:AN002243
Instrument Name:Sciex 6500+ QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002090
Analysis ID:AN002244
Instrument Name:Sciex 6500+ QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
  
MS ID:MS002091
Analysis ID:AN002245
Instrument Name:Sciex 6500+ QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
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