Summary of Study ST001360

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000930. The data can be accessed directly via it's Project DOI: 10.21228/M8GH4X This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001360
Study TitleMaternal blood lipidomics associated with severe preeclampsia
Study TypeHuman sample study
Study SummaryQuantiative lipidomic (753 lipid species) study on blood samples (66 samples) from severe preeclampsia (44 samples) and control (20 samples) donors
Institute
University of Michigan
DepartmentDepartment of Computational Medicine and Bioinformatics
LaboratoryLana Garmire Lab
Last NameLiu
First NameYu
Address1600 Huron Parkway, NCRC Building 520, Ann Arbor, MI 48105
Emailuiluy@umich.edu
Phone973-817-1360
Submit Date2020-03-31
Num Groups2
Total Subjects64
Num Females64
Analysis Type DetailLC-MS
Release Date2022-04-01
Release Version1
Yu Liu Yu Liu
https://dx.doi.org/10.21228/M8GH4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000930
Project DOI:doi: 10.21228/M8GH4X
Project Title:Maternal blood lipidomics associated with severe preeclampsia
Project Type:MS quantitative analysis
Project Summary:Quantiative lipidomic (753 lipid species) study on blood samples (66 samples) from severe preeclampsia (44 samples) and control (20 samples) donors
Institute:University of Michigan
Department:Department of Computational Medicine and Bioinformatics
Laboratory:Lana Garmire Lab
Last Name:Liu
First Name:Yu
Address:1600 Huron Parkway, NCRC Building 520, Ann Arbor, MI, 48105, USA
Email:uiluy@umich.edu
Phone:973-817-1360

Subject:

Subject ID:SU001434
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample_Group
SA098899Control_7112Control
SA098900Control_7146Control
SA098901Control_7081Control
SA098902Control_6763Control
SA098903Control_6598Control
SA098904Control_4978Control
SA098905Control_7504Control
SA098906Control_8866Control
SA098907Control_1950Control
SA098908Control_7956Control
SA098909Control_7732Control
SA098910Control_6396Control
SA098911Control_7488Control
SA098912Control_7305Control
SA098913Control_6230Control
SA098914Control_5787Control
SA098915Control_5133Control
SA098916Control_5622Control
SA098917Control_6292Control
SA098918Control_5258Control
SA098919Preeclampsia_4365Preeclampsia
SA098920Preeclampsia_5237Preeclampsia
SA098921Preeclampsia_6161Preeclampsia
SA098922Preeclampsia_6971Preeclampsia
SA098923Preeclampsia_7596Preeclampsia
SA098924Preeclampsia_6213Preeclampsia
SA098925Preeclampsia_5525Preeclampsia
SA098926Preeclampsia_5512Preeclampsia
SA098927Preeclampsia_5346Preeclampsia
SA098928Preeclampsia_7808Preeclampsia
SA098929Preeclampsia_5228Preeclampsia
SA098930Preeclampsia_9064Preeclampsia
SA098931Preeclampsia_5851Preeclampsia
SA098932Preeclampsia_5521Preeclampsia
SA098933Preeclampsia_4877Preeclampsia
SA098934Preeclampsia_8557Preeclampsia
SA098935Preeclampsia_7012Preeclampsia
SA098936Preeclampsia_6677Preeclampsia
SA098937Preeclampsia_7655Preeclampsia
SA098938Preeclampsia_5524Preeclampsia
SA098939Preeclampsia_7393Preeclampsia
SA098940Preeclampsia_5541Preeclampsia
SA098941Preeclampsia_7570Preeclampsia
SA098942Preeclampsia_5073Preeclampsia
SA098943Preeclampsia_5764Preeclampsia
SA098944Preeclampsia_6350Preeclampsia
SA098945Preeclampsia_6508Preeclampsia
SA098946Preeclampsia_8107Preeclampsia
SA098947Preeclampsia_6603Preeclampsia
SA098948Preeclampsia_5035Preeclampsia
SA098949Preeclampsia_7395Preeclampsia
SA098950Preeclampsia_5094Preeclampsia
SA098951Preeclampsia_7297Preeclampsia
SA098952Preeclampsia_6770Preeclampsia
SA098953Preeclampsia_5447Preeclampsia
SA098954Preeclampsia_6780Preeclampsia
SA098955Preeclampsia_7825Preeclampsia
SA098956Preeclampsia_7376Preeclampsia
SA098957Preeclampsia_7563Preeclampsia
SA098958Preeclampsia_7468Preeclampsia
SA098959Preeclampsia_6127Preeclampsia
SA098960Preeclampsia_7565Preeclampsia
SA098961Preeclampsia_7242Preeclampsia
SA098962Preeclampsia_7718Preeclampsia
Showing results 1 to 64 of 64

Collection:

Collection ID:CO001429
Collection Summary:We obtained samples from RMATRIX Hawaii Biorepository (HiBR), which obtained its own IRB approval. 44 maternal plasma samples from clinically diagnosed severe preeclampsia patients and 20 control samples (full-term deliveries without complications such as gestational diabetes) were selected.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001449
Treatment Summary:We obtained samples from RMATRIX Hawaii Biorepository (HiBR), which obtained its own IRB approval. 44 maternal plasma samples from clinically diagnosed severe preeclampsia patients and 20 control samples (full-term deliveries without complications such as gestational diabetes) were selected. Plasma samples were stored at − 80 °C until the time of analysis, which was conducted by the Michigan Regional Comprehensive Metabolomics Resource Core.

Sample Preparation:

Sampleprep ID:SP001442
Sampleprep Summary:Plasma samples were stored at − 80 °C until the time of analysis, which was conducted by the Michigan Regional Comprehensive Metabolomics Resource Core. Lipids were extracted using a modified BlighDyer Method. The extraction was performed using water/methanol/dichloromethane (2:2:2 v/v/v) at room temperature after the addition of internal standards. The organic layer was then collected and dried under a stream of nitrogen before being re-suspended in 100 μL of Buffer B (5%H2O:10%ACN:85%IPA containing 10 mM NH4OAc) and analyzed using a liquid chromatography tandem mass spectrometry (LC/MS/MS) lipidomics assay. The lipid extract was injected onto a 1.8 μm particle 50 × 2.1 mm internal diameter Waters Acquity HSS T3 column (Waters, Milford, MA) that was heated to 55 °C. For chromatographic elution, we used a linear gradient over a 20 min total run time. A 60% Solvent A and 40% Solvent B gradient was used for the first 10 min. Then the gradient was ramped in a linear fashion to 100% Solvent B which was maintained for 7 min. Thereafter, the system was switched back to 60% Solvent B and 40% Solvent A for 3 min. The flow rate used for these experiments was 0.4 mL/min and the injection volume was 5 μL. The column was equilibrated for 3 min before the next injection and run at a flow rate of 0.400uL/min for a total run time of 20 min. Data were acquired in positive and negative modes using data-dependent MS/MS with dynamic mass exclusion. Pooled human plasma samples and pooled experimental samples (prepared by combining small aliquots of each experimental sample) were used to control for the quality of sample preparation and analysis. A randomization scheme was used to distribute pooled samples within the set and a mixture of pure authentic standards was used to monitor instrument performance on a regular basis.
Processing Storage Conditions:-80℃
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN002264
Analysis type MS
Chromatography type HILIC
Chromatography system Waters Acquity H-Class
Column Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Ion trap
MS instrument name Waters Micromass QTOF Premier
Ion Mode UNSPECIFIED
Units normalized

Chromatography:

Chromatography ID:CH001663
Instrument Name:Waters Acquity H-Class
Column Name:Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:HILIC

MS:

MS ID:MS002108
Analysis ID:AN002264
Instrument Name:Waters Micromass QTOF Premier
Instrument Type:Ion trap
MS Type:ESI
MS Comments:Lipids were identified using the LIPIDBLAST computer-generated tandem MS library. This database contains 212,516 spectra covering 119,200 compounds representing 26 lipid classes, including phospholipids, glycerolipids, bacterial lipoglycan, and plant glycolipids. Quantification of lipids was completed using ABSCIEX MultiQuant software. Compounds with a relative standard deviation greater than 30% in the pooled samples were excluded.
Ion Mode:UNSPECIFIED
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