Summary of Study ST001379
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000916. The data can be accessed directly via it's Project DOI: 10.21228/M8912S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001379 |
| Study Title | 2,3,7,8 -Tetrachlorodibenzo-p-dioxin Mediated Effects on Hepatic Coenzyme A (CoA) and Acetyl-CoA Levels (part-II) |
| Study Type | Dioxin |
| Study Summary | Experiment to study TCDD-elicited effects on hepatic Coenzyme A (CoA) and Acetyl-CoA levels in liver of male mice treated with sesame oil vehicle or 0.01-30 ug/kg TCDD every 4 days for 28 days. |
| Institute | Michigan State University |
| Department | Biochemistry and Molecular Biology |
| Laboratory | Zacharewski Lab |
| Last Name | Fling |
| First Name | Russell |
| Address | 1129 Farm Ln, East Lansing, MI, USA |
| flingrus@msu.edu | |
| Phone | (517) 884-2054 |
| Submit Date | 2020-04-01 |
| Num Groups | 9 |
| Total Subjects | 80 |
| Num Males | 29 |
| Study Comments | Fax: (517) 353-9334; ORCID: 0000-0002-6822-4962; ROLE: Graduate Student; Pubmed ID: -;Publication DOI: -; |
| Publications | - |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000916 |
| Project DOI: | doi: 10.21228/M8912S |
| Project Title: | TCDD-elicited effects on metabolite levels in liver of male mice treated with sesame oil |
| Project Type: | Untargetd Metabolomics |
| Project Summary: | Experiment to study TCDD-elicited effects on metabolite levels in liver of male mice treated with sesame oil vehicle or 0.01-30 ug/kg TCDD every 4 days for 28 days. |
| Institute: | Michigan State University |
| Department: | Biochemistry and Molecular Biology |
| Last Name: | Fling |
| First Name: | Russell |
| Address: | 1129 Farm Ln, East Lansing, MI,USA |
| Email: | flingrus@msu.edu |
| Phone: | (517) 884-2054 |
| Publications: | - |
Subject:
| Subject ID: | SU001453 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | postnatal day 56 |
| Gender: | Male |
| Animal Housing: | Cage Type: Innovive, Catalog#M-BTM, San Diego, CA; Bedding Type: cellulose fiber chips; |
| Animal Light Cycle: | 12:12 |
| Animal Feed: | 22/5 Rodent Diet |
| Animal Water: | Aquavive, Catalog#M-WB-300A, INNOVIVE, San Diego, CA |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | TCDD treatment(ug/kg) |
|---|---|---|
| SA100866 | mouse_33 | 0.3 |
| SA100867 | mouse_35 | 0.3 |
| SA100868 | mouse_38 | 0.3 |
| SA100869 | mouse_34 | 0.3 |
| SA100870 | mouse_37 | 0.3 |
| SA100871 | mouse_43 | 1 |
| SA100872 | mouse_46 | 1 |
| SA100873 | mouse_44 | 1 |
| SA100874 | mouse_42 | 1 |
| SA100875 | mouse_48 | 1 |
| SA100881 | mouse_61 | 10 |
| SA100882 | mouse_64 | 10 |
| SA100883 | mouse_63 | 10 |
| SA100884 | mouse_60 | 10 |
| SA100876 | mouse_49 | 3 |
| SA100877 | mouse_59 | 3 |
| SA100878 | mouse_53 | 3 |
| SA100879 | mouse_56 | 3 |
| SA100880 | mouse_50 | 3 |
| SA100885 | mouse_67 | 30 |
| SA100886 | mouse_68 | 30 |
| SA100887 | mouse_69 | 30 |
| SA100888 | mouse_66 | 30 |
| SA100889 | mouse_65 | 30 |
| SA100890 | mouse_2 | vehicle |
| SA100891 | mouse_1 | vehicle |
| SA100892 | mouse_6 | vehicle |
| SA100893 | mouse_7 | vehicle |
| SA100894 | mouse_8 | vehicle |
| Showing results 1 to 29 of 29 |
Collection:
| Collection ID: | CO001448 |
| Collection Summary: | Mice were taken down with CO2. Livers were excised and immediately frozen in liquid nitrogen. |
| Collection Protocol Comments: | Time-point: 28; Time-point Unit: DAYS; |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001468 |
| Treatment Summary: | oral gavage every 4 days for 28 days. 7 total exposures with sesame oil vehicle or 0.3-30 ug/kg TCDD. |
| Treatment Protocol Comments: | Administration Interval: 4; Administration Interval Unit: DAYS; Dose Unit: ug/kg; Number of Administrations: 7; Chemical Soucre: AccuStandard; Chemical Catalog Number: S-70982; Chemical Purity: -; Vehicle Substance: CHEBI 28119 |
| Treatment Compound: | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) |
| Treatment Route: | GAVAGE |
| Treatment Dose: | 0.3,1,3,10,30 |
| Treatment Dosevolume: | 0.1 ml |
| Treatment Vehicle: | Sesame oil |
| Animal Fasting: | 2 hrs before end-point |
| Animal Endp Euthanasia: | CO2 |
Sample Preparation:
| Sampleprep ID: | SP001461 |
| Sampleprep Summary: | Frozen liver tissue was homegenized in 4.6 ml h20:methanol (5:3) mixture and homegenized, 2.9 ml chloroform was added,vortexed and spun down at 5,000G. Top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
| Processing Method: | Liquid extraction |
| Processing Storage Conditions: | -80℃ |
Chromatography:
| Chromatography ID: | CH001689 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Ascentis Express C18 (2.1 x 5 cm) |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN002299 |
| Analysis Type: | MS |
| Operator Name: | Russ Fling |
| Chromatography ID: | CH001689 |
| Num Factors: | 6 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST001379_AN002299_Results.txt |
| Units: | normalized signal area |
