Summary of study ST001398

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000958. The data can be accessed directly via it's Project DOI: 10.21228/M8VM30 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001398
Study TitleMechanism of Trichloroethylene (TCE) toxicity in the placenta
Study TypeMS analysis
Study SummaryCells in culture will be exposed to S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a relevant metabolite of TCE. The first cell line used will be HTR-8/SVneo, originally derived from first trimester female human cytotrophoblast cells and immortalized with simian virus 40 large T antigen, this cell line models first-trimester placental extravillous trophoblasts in vitro. HTR-8/SVneo cells will be cultured for 24 hours followed by exposure to cell culture media (control) or 20 µM DCVC for 6 or 12 hours. Cells (cells frozen in cell culture dish) will then be collected (n=5) In the TCE aim of this study, we will utilize one cell model that phenotypically represents an important placental cell population.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2020-06-08
Publicationshttps://pubmed.ncbi.nlm.nih.gov/31951115/
Raw Data AvailableYes
Raw Data File Type(s).cd, .cg
Analysis Type DetailLC-MS
Release Date2020-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8VM30
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000958
Project DOI:doi: 10.21228/M8VM30
Project Title:Mechanism of Trichloroethylene (TCE) toxicity in placenta
Project Summary:This study will provide important information on the mechanism of TCE toxicity in the placenta
Institute:University of Michigan
Department:Environmental Health
Laboratory:Loch-Caruso Lab
Last Name:Loch-Caruso
First Name:Rita
Address:Ann Arbor, MI
Email:rlc@umich.edu
Phone:734-936-1256

Subject:

Subject ID:SU001472
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Hours of Exposure Treatment
SA113568S0003491112 hr 20 uM DCVC
SA113569S0003491212 hr 20 uM DCVC
SA113570S0003491312 hr 20 uM DCVC
SA113571S0003491412 hr 20 uM DCVC
SA113572S0003491512 hr 20 uM DCVC
SA113573S0003490812 hr No Treatment
SA113574S0003490912 hr No Treatment
SA113575S0003491012 hr No Treatment
SA113576S0003490712 hr No Treatment
SA113577S0003490612 hr No Treatment
SA113578S000349016 hr 20 uM DCVC
SA113579S000349036 hr 20 uM DCVC
SA113580S000349026 hr 20 uM DCVC
SA113581S000349046 hr 20 uM DCVC
SA113582S000349056 hr 20 uM DCVC
SA113583S000348976 hr No Treatment
SA113584S000348986 hr No Treatment
SA113585S000349006 hr No Treatment
SA113586S000348966 hr No Treatment
SA113587S000348996 hr No Treatment
Showing results 1 to 20 of 20

Collection:

Collection ID:CO001467
Collection Summary:Standard tissue culture sample collection method
Sample Type:Cultured cells

Treatment:

Treatment ID:TR001487
Treatment Summary:Exposure to cell culture media (control) or 20 µM S-(1,2-dichlorovinyl)-L-cysteine (DCVC) for 6 or 12 hours

Sample Preparation:

Sampleprep ID:SP001480
Sampleprep Summary:Standard TCS-plus assay sample preparation protocol
Sampleprep Protocol Filename:SP_A043_TCA_Plus_20170713.PDF

Combined analysis:

Analysis ID AN002337 AN002338
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1200 Agilent 1200
Column Phenomenex Luna NH2 (150 x 2.1mm, 3um) Phenomenex Luna NH2 (150 x 2.1mm, 3um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6520 QTOF Agilent 6520 QTOF
Ion Mode NEGATIVE NEGATIVE
Units area/ug protein pmol/ug

Chromatography:

Chromatography ID:CH001713
Chromatography Summary:HILIC NH2 chromatography
Instrument Name:Agilent 1200
Column Name:Phenomenex Luna NH2 (150 x 2.1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS002179
Analysis ID:AN002337
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Quantitative data analysis using Agilent Quant software
Ion Mode:NEGATIVE
  
MS ID:MS002180
Analysis ID:AN002338
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Semi-quantitative data analysis using Agilent Quant software
Ion Mode:NEGATIVE
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