Summary of Study ST001488

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001003. The data can be accessed directly via it's Project DOI: 10.21228/M8210V This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001488
Study TitleMetabolomics of Ndufs4 KO brain regions (part - VIII)
Study SummaryUntargeted GC-TOF-MS analysis of Ndufs4 KO and WT mouse olfactory bulbs (OB)
North-West University
Last NameLouw
First NameRoan
AddressHofman Street
Phone+27 18 299 4074
Submit Date2020-09-11
Raw Data AvailableYes
Raw Data File Type(s)peg
Analysis Type DetailGC-MS
Release Date2021-09-10
Release Version1
Roan Louw Roan Louw application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR001003
Project DOI:doi: 10.21228/M8210V
Project Title:Ndufs4 KO mouse model metabolomics studies
Project Type:Multi-platform metabolomics analysis
Project Summary:Multi-platform metabolomics analysis of tissues and biofluids from the Ndufs4 knockout (Ndufs4-/-) mouse model of human Leigh syndrome
Institute:North-West University
Last Name:Louw
First Name:Roan
Address:Hofman Street
Phone:+27 18 299 4074


Subject ID:SU001562
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Ndufs4, n/02705 8
Age Or Age Range:45-50 days
Animal Animal Supplier:Jackson Laboratory (ME, USA)
Animal Light Cycle:12:12 h
Animal Feed:Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub
Animal Water:ad libitum


Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA125373OB1 19KO
SA125374OB1 21KO
SA125375OB2 27KO
SA125376OB1 16KO
SA125377OB1 24KO
SA125378OB1 15KO
SA125379OB1 3KO
SA125380OB1 10KO
SA125381OB1 14KO
SA125382OB2 29KO
SA125383OB2 32KO
SA125384OB2 41KO
SA125385OB2 42KO
SA125386OB2 E 4KO
SA125387OB2 38KO
SA125388OB2 36KO
SA125389OB2 33KO
SA125390OB2 34KO
SA125391OB2 35KO
SA125392OB2 40WT
SA125393OB2 39WT
SA125394OB1 9WT
SA125395OB1 11WT
SA125396OB1 12WT
SA125397OB1 13WT
SA125398OB1 8WT
SA125399OB1 7WT
SA125400OB1 2WT
SA125401OB1 4WT
SA125402OB1 5WT
SA125403OB1 17WT
SA125404OB1 18WT
SA125405OB2 28WT
SA125406OB2 30WT
SA125407OB2 31WT
SA125408OB2 37WT
SA125409OB2 26WT
SA125410OB2 25WT
SA125411OB1 20WT
SA125412OB1 22WT
SA125413OB1 23WT
SA125414OB1 1WT
Showing results 1 to 42 of 42


Collection ID:CO001557
Collection Summary:Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. The brain was removed and rinsed with saline solution (SABAX PBS; 0.9% NaCl (w/v), #7634, Adcock Ingram) to remove surrounding blood. The brain regions of interest, namely the anterior cortex (AC), brainstem (BS), cerebellum (CB) and olfactory bulbs (OB), were then dissected, snap-frozen in liquid nitrogen (within 15 minutes postmortem) and stored at − 80°C until used.
Sample Type:Brain


Treatment ID:TR001577
Treatment Summary:The animals did not receive any treatment

Sample Preparation:

Sampleprep ID:SP001570
Sampleprep Summary:BBrain regions were homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform).
Sampleprep Protocol Filename:GC_sample_prep_protocol.pdf

Combined analysis:

Analysis ID AN002465
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco GC-TOF
Units area


Chromatography ID:CH001807
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:GC


MS ID:MS002285
Analysis ID:AN002465
Instrument Name:Leco GC-TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:The LECO Corporation ChromaTOF® software (v 4.5x) was used for data acquisition and extraction. This included automatic baseline removal via the “spanning” tracking method (offset of 1; just above the noise) and auto smoothing, with the software’s Statistical Compare feature used to align peaks. Spectral matching was done using the NIST11 commercial library and an in-house mass spectral library in order to identify important analytes.