Summary of study ST001648

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001054. The data can be accessed directly via it's Project DOI: 10.21228/M8FX20 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001648
Study TitleComprehensive dissection of primary metabolites in response to diverse abiotic stress in barley at seedling stage
Study TypeArtical
Study SummaryPlants will meet various abiotic stresses during their growth and development. One of the important strategies for plants to deal with the stress is involved in metabolic regulation, causing the dramatic changes of metabolite profiles. Metabolomic studies have been intensively conducted to reveal the responses of plants to abiotic stress, but most of them were limited to one or at most two abiotic stresses in a single experiment. In this study, we compared the metabolite profiles of barley seedlings exposed to seven abiotic stresses simultaneously, including drought, salt stress, aluminum (Al), cadmium (Cd), deficiency of nitrogen (N), phosphorus (P) and potassium (K). The results showed that metabolite profiles of barley under these stresses could be classified into three types: osmotic stresses (drought and salt); metal stresses (Al and Cd) and nutrient deficiencies (N, P and K deficiencies). Compared with the control, some metabolites (including polyamines, raffinose and piperonic acid) in plants exposed to all abiotic stresses changed significantly, while some other metabolites showed the specific change only under a certain abiotic stress, such as proline being largely increased by osmotic stress (drought and salinity), the P-containing metabolites being largely decreased under P deficiency, some amino acids (lysine, tyrosine, threonine, ornithine, glutamine and so on) showing the dramatic reduction in the plants exposed to N deficiencies, respectively. The current meta-analysis obtained a comprehensive view on the metabolic responses to various abiotic stress, and improved the understanding of the mechanisms for tolerance of barley to abiotic stress.
Institute
Zhengjiang University
DepartmentCrop Research Institute
LaboratoryBarley Research Group of Crop Institute
Last NameZhao
First NameHuifang
AddressWest Lake District, 866 Yuhangtang Road
Email11716028@zju.edu.cn
Phone18888923662
Submit Date2021-01-07
Num Groups1
Total Subjects1
Num Males2
Num Females1
PublicationsPLANT PHYSIOLOGY AND BIOCHEMISTRY
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailAPI-MS
Release Date2022-01-08
Release Version1
Huifang Zhao Huifang Zhao
https://dx.doi.org/10.21228/M8FX20
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001054
Project DOI:doi: 10.21228/M8FX20
Project Title:Comprehensive dissection of primary metabolites in response to diverse abiotic stress in barley at seedling stage
Project Type:Artical
Project Summary:Plants will meet various abiotic stresses during their growth and development. One of the important strategies for plants to deal with the stress is involved in metabolic regulation, causing the dramatic changes of metabolite profiles. Metabolomic studies have been intensively conducted to reveal the responses of plants to abiotic stress, but most of them were limited to one or at most two abiotic stresses in a single experiment. In this study, we compared the metabolite profiles of barley seedlings exposed to seven abiotic stresses simultaneously, including drought, salt stress, aluminum (Al), cadmium (Cd), deficiency of nitrogen (N), phosphorus (P) and potassium (K). The results showed that metabolite profiles of barley under these stresses could be classified into three types: osmotic stresses (drought and salt); metal stresses (Al and Cd) and nutrient deficiencies (N, P and K deficiencies). Compared with the control, some metabolites (including polyamines, raffinose and piperonic acid) in plants exposed to all abiotic stresses changed significantly, while some other metabolites showed the specific change only under a certain abiotic stress, such as proline being largely increased by osmotic stress (drought and salinity), the P-containing metabolites being largely decreased under P deficiency, some amino acids (lysine, tyrosine, threonine, ornithine? glutamine and so on) showing the dramatic reduction in the plants exposed to N deficiencies, respectively. The current meta-analysis obtained a comprehensive view on the metabolic responses to various abiotic stress, and improved the understanding of the mechanisms for tolerance of barley to abiotic stress.
Institute:Zhengjiang University
Department:Crop Research Institute
Laboratory:Barley Research Group of Crop Institute
Last Name:Zhao
First Name:Huifang
Address:West Lake District, 866 Yuhangtang Road
Email:11716028@zju.edu.cn
Phone:18888923662
Funding Source:National Key R&D Program of China; Natural Science Foundation of China; China Agriculture Research System
Publications:PLANT PHYSIOLOGY AND BIOCHEMISTRY

Subject:

Subject ID:SU001725
Subject Type:Plant
Subject Species:Hordeum vulgare L.
Taxonomy ID:4513

Factors:

Subject type: Plant; Subject species: Hordeum vulgare L. (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA151599root-13Root_Al-1
SA151600root-14Root_Al-2
SA151601root-15Root_Al-3
SA151602root-16Root_Al-4
SA151603root-17Root_Cd-1
SA151604root-18Root_Cd-2
SA151605root-19Root_Cd-3
SA151606root-20Root_Cd-4
SA151607root-1Root_Control-1
SA151608root-2Root_Control-2
SA151609root-3Root_Control-3
SA151610root-4Root_Control-4
SA151611root-9Root_Drought-1
SA151612root-10Root_Drought-2
SA151613root-11Root_Drought-3
SA151614root-12Root_Drought-4
SA151615root-29Root_K deficiency-1
SA151616root-30Root_K deficiency-2
SA151617root-31Root_K deficiency-3
SA151618root-32Root_K deficiency-4
SA151619root-21Root_N deficiency-1
SA151620root-22Root_N deficiency-2
SA151621root-23Root_N deficiency-3
SA151622root-24Root_N deficiency-4
SA151623root-25Root_P deficiency-1
SA151624root-26Root_P deficiency-2
SA151625root-27Root_P deficiency-3
SA151626root-28Root_P deficiency-4
SA151627root-5Root_Salt-1
SA151628root-6Root_Salt-2
SA151629root-7Root_Salt-3
SA151630root-8Root_Salt-4
SA151631shoot-13Shoot_Al-1
SA151632shoot-14Shoot_Al-2
SA151633shoot-15Shoot_Al-3
SA151634shoot-16Shoot_Al-4
SA151635shoot-17Shoot_Cd-1
SA151636shoot-18Shoot_Cd-2
SA151637shoot-19Shoot_Cd-3
SA151638shoot-20Shoot_Cd-4
SA151639shoot-1Shoot_Control-1
SA151640shoot-2Shoot_Control-2
SA151641shoot-3Shoot_Control-3
SA151642shoot-4Shoot_Control-4
SA151643shoot-9Shoot_Drought-1
SA151644shoot-10Shoot_Drought-2
SA151645shoot-11Shoot_Drought-3
SA151646shoot-12Shoot_Drought-4
SA151647shoot-29Shoot_K deficiency-1
SA151648shoot-30Shoot_K deficiency-2
SA151649shoot-31Shoot_K deficiency-3
SA151650shoot-32Shoot_K deficiency-4
SA151651shoot-21Shoot_N deficiency-1
SA151652shoot-22Shoot_N deficiency-2
SA151653shoot-23Shoot_N deficiency-3
SA151654shoot-24Shoot_N deficiency-4
SA151655shoot-25Shoot_P deficiency-1
SA151656shoot-26Shoot_P deficiency-2
SA151657shoot-27Shoot_P deficiency-3
SA151658shoot-28Shoot_P deficiency-4
SA151659shoot-5Shoot_Salt-1
SA151660shoot-6Shoot_Salt-2
SA151661shoot-7Shoot_Salt-3
SA151662shoot-8Shoot_Salt-4
Showing results 1 to 64 of 64

Collection:

Collection ID:CO001718
Collection Summary:Barley seedlings were allowed to grow up to two-leaf-stage in the normal hydroponic solution, and then exposed to various treatments after removing endosperm for eliminating its nutrient supply. The treatments included 20% PEG for drought stress, 150 mM NaCl for salt stress, 50 μM Al3+ at pH 4.5 for aluminum stress (Al stress, at the same time the nutrient solution with pH 4.5 and without Al addition used as control), 5 μM CdCl2 for cadmium stress (Cd stress), 0 mM N for N deficiency (replace KNO3, Ca(NO3).4H2O and (NH4)6Mo7O24 with 1mM CaCl2, 1mM KH2PO4 and 0.07 mM H2MoO4), 0 mM P for P deficiency (replace NH4H2PO4 with 1mM NH4Cl), 0 mM K for K deficiency (replace KNO3 with 1mM NaNO3). The hydroponic solution was renewed every four days. After twenty days of the treatments, the plants were harvested, and fresh and dry weight were recorded quickly. The dry tissues were used for mineral element concentration analysis with three biological replicates for each treatment. Meanwhile, fresh tissues were frozen in liquid nitrogen and stored at -80 ℃ for metabolite analysis with four biological replicates for each stress treatment.
Sample Type:Plant
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001738
Treatment Summary:The treatments included 20% PEG for drought stress, 150 mM NaCl for salt stress, 50 μM Al3+ at pH 4.5 for aluminum stress (Al stress, at the same time the nutrient solution with pH 4.5 and without Al addition used as control), 5 μM CdCl2 for cadmium stress (Cd stress), 0 mM N for N deficiency (replace KNO3, Ca(NO3).4H2O and (NH4)6Mo7O24 with 1mM CaCl2, 1mM KH2PO4 and 0.07 mM H2MoO4), 0 mM P for P deficiency (replace NH4H2PO4 with 1mM NH4Cl), 0 mM K for K deficiency (replace KNO3 with 1mM NaNO3).

Sample Preparation:

Sampleprep ID:SP001731
Sampleprep Summary:The frozen tissue was homogenized in liquid nitrogen and approximately 100 mg of powder sample was added into 2 ml vertical centrifuge tube. Then 1.4 ml of 100% methanol (pre-cooled at -20 °C) was added and vortexed thoroughly. Then the tubes were shaken for 10 min at 70 °C, followed by centrifugation at 11,000 g for 10 min. The supernatant was transferred into a new 10 ml sterile centrifuge tube, and 750 μl chloroform (−20 °C) and 1.5 ml deionized water (4 °C) were added. The mixture was vortexed and centrifuged at 2200 g for 15 min. The upper methanol/water phase was collected for metabolite analysis. A total of 150 μl collected solution was dried in a vacuum freeze dryer. The dried residue was derivatized in 40 μl of 15 mg ml-1 methoxylamine hydrochloride in pyridine for 120 min at 37 °C, followed by reaction with 70 μl of N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) reagent for another 30 min at 37 °C. After the reaction, the reacted solution was transferred to glass vial insert in 2 ml glass bottle (Agilent, USA) for GC-MS determination. Meanwhile, one blank derivatization reaction (with reagent) was also prepared as a control.

Combined analysis:

Analysis ID AN002694
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type API
MS instrument type GC-TOF
MS instrument name Agilent 7890A
Ion Mode UNSPECIFIED
Units pmoles/l

Chromatography:

Chromatography ID:CH001987
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS002492
Analysis ID:AN002694
Instrument Name:Agilent 7890A
Instrument Type:GC-TOF
MS Type:API
MS Comments:The retention time (RT) and mass spectra of each compound were analyzed using AMDIS (Automated Mass Spectral Deconvolution and Identification System) software (http://chemdata.nist.gov/).
Ion Mode:UNSPECIFIED
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