Summary of Study ST001782

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001113. The data can be accessed directly via it's Project DOI: 10.21228/M8TX2D This work is supported by NIH grant, U2C- DK119886.

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Study IDST001782
Study TitleExamining the Identified Differential Metabolites in Other Antipsychotics with a High Fatality Frequency (part IV)
Study Typeoriginal study
Study SummaryExamining the Identified Differential Metabolites (acetyl-L-carnitine, propionyl-L-carnitine and succinic acid) in Other Antipsychotics.
Institute
Hebei medical university
Last NameBai
First NameRui
AddressNo.9 Tiyu North Street, Chang'an District
Email15822925144@163.com
Phone18522889554
Submit Date2021-04-04
Analysis Type DetailLC-MS
Release Date2025-03-30
Release Version1
Rui Bai Rui Bai
https://dx.doi.org/10.21228/M8TX2D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001113
Project DOI:doi: 10.21228/M8TX2D
Project Title:Examination of Candidate Differential Metabolites for Lethal Chlorpromazine Poisoning Using a LC-MS-based Metabolomics Approach in Mice
Project Type:Original Research
Project Summary:With the innovation of metabolite detection technology and the application of chemometrics in biomarker identification, metabolomics has been widely used to identify endogenous differential metabolites after drug poisoning. In this study, ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was combined with advanced chemometric approaches to identify differential metabolites that are associated with lethal chlorpromazine (CPZ) poisoning and associated perturbed metabolic pathways. Differential metabolite specificity and stability were assessed by comparing the CPZ samples to other antipsychotics, such as perphenazine (PER), clozapine (CLO) and olanzapine (OLA), that are associated with a high fatality frequency and by comparing them to non-drug related deaths (NDRDs) associated with hypoxia. The results implicated three candidate differential metabolites (acetyl-L-carnitine, propionyl-L-carnitine and succinic acid) with a high and relatively stable sensitivity (85.7–100%) following predictive analysis. Additionally, no differential metabolites were identified when comparing CPZ to PRE, OLA and CLO, but all of the drugs showed a similar pharmacodynamic receptor profile. Overall, this study provides a methodological and theoretical basis for biomarker identification in lethal CPZ poisoning (LCP) cases.
Institute:new
Last Name:Bai
First Name:rui
Address:Hebei Province, Shijiazhuang 050018, P.R. China
Email:15822925144@163.com
Phone:18522889554

Subject:

Subject ID:SU001859
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:7-8 weeks
Gender:Male and female
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Phenotype 1 Phenotype 2 Phenotype 3
SA165159FT4Cervical dislocation Non-drug related death Non-drug related death
SA165160FT3Cervical dislocation Non-drug related death Non-drug related death
SA165161FT2Cervical dislocation Non-drug related death Non-drug related death
SA165162MT1Cervical dislocation Non-drug related death Non-drug related death
SA165163MT3Cervical dislocation Non-drug related death Non-drug related death
SA165164MT5Cervical dislocation Non-drug related death Non-drug related death
SA165165MT4Cervical dislocation Non-drug related death Non-drug related death
SA165166FT1Cervical dislocation Non-drug related death Non-drug related death
SA165167MT2Cervical dislocation Non-drug related death Non-drug related death
SA165168FT5Cervical dislocation Non-drug related death Non-drug related death
SA165169FN5Drowning Non-drug related death Non-drug related death
SA165170FN4Drowning Non-drug related death Non-drug related death
SA165171FN3Drowning Non-drug related death Non-drug related death
SA165172MN1Drowning Non-drug related death Non-drug related death
SA165173MN2Drowning Non-drug related death Non-drug related death
SA165174MN5Drowning Non-drug related death Non-drug related death
SA165175MN4Drowning Non-drug related death Non-drug related death
SA165176MN3Drowning Non-drug related death Non-drug related death
SA165177FN2Drowning Non-drug related death Non-drug related death
SA165178FN1Drowning Non-drug related death Non-drug related death
SA165179FX1Hemorrhagic shock Non-drug related death Non-drug related death
SA165180FX4Hemorrhagic shock Non-drug related death Non-drug related death
SA165181FX3Hemorrhagic shock Non-drug related death Non-drug related death
SA165182FX5Hemorrhagic shock Non-drug related death Non-drug related death
SA165183MX2Hemorrhagic shock Non-drug related death Non-drug related death
SA165184MX5Hemorrhagic shock Non-drug related death Non-drug related death
SA165185MX4Hemorrhagic shock Non-drug related death Non-drug related death
SA165186MX3Hemorrhagic shock Non-drug related death Non-drug related death
SA165187FX2Hemorrhagic shock Non-drug related death Non-drug related death
SA165188MX1Hemorrhagic shock Non-drug related death Non-drug related death
SA165189FLS2Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165190FLS5Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165191MLS5Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165192FLS4Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165193MLS1Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165194MLS2Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165195MLS4Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165196FLS3Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165197MLS3Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165198FLS1Lethal CLO poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165199MCS5Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165200FCS1Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165201MCS2Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165202MCS3Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165203MCS4Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165204MCS1Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165205FCS5Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165206FCS3Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165207FCS4Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165208FCS2Lethal CPZ poisoning Lethal CPZ poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165209MOS2Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165210MOS4Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165211MOS5Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165212FOS2Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165213MOS3Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165214MOS1Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165215FOS3Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165216FOS4Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165217FOS5Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165218FOS1Lethal OLA poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165219FFS1Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165220FFS4Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165221FFS3Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165222FFS2Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165223FFS5Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165224MFS1Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165225MFS4Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165226MFS3Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165227MFS2Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165228MFS5Lethal PER poisoning PER, OLA, CLO lethal poisoning CPZ, PER, OLA, CLO lethal poisoning
SA165229FZ1Mechanical asphyxia Non-drug related death Non-drug related death
SA165230FZ4Mechanical asphyxia Non-drug related death Non-drug related death
SA165231FZ3Mechanical asphyxia Non-drug related death Non-drug related death
SA165232FZ2Mechanical asphyxia Non-drug related death Non-drug related death
SA165233FZ5Mechanical asphyxia Non-drug related death Non-drug related death
SA165234MZ1Mechanical asphyxia Non-drug related death Non-drug related death
SA165235MZ4Mechanical asphyxia Non-drug related death Non-drug related death
SA165236MZ3Mechanical asphyxia Non-drug related death Non-drug related death
SA165237MZ2Mechanical asphyxia Non-drug related death Non-drug related death
SA165238MZ5Mechanical asphyxia Non-drug related death Non-drug related death
Showing results 1 to 80 of 80

Collection:

Collection ID:CO001852
Collection Summary:plasma were isolated and then flash-frozen in liquid N2
Collection Protocol Filename:process_protocol.pdf
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR001872
Treatment Summary:Lethal poisoning animals were given different antisycotics (chlorpromazine，Olanzapine, perphenazine and clozapine) through gavage.Non-drug related death animal models according to previous study.Abdominal aortic blood was obtained after death.
Treatment Protocol Filename:process_protocol.pdf
Treatment:drug
Treatment Compound:chlorpromazine,Olanzapine, perphenazine and clozapine
Treatment Route:gavage
Treatment Dose:150mg/100g,80mg/100g,150mg/100g,80mg/100g,
Treatment Dosevolume:300ul
Treatment Doseduration:once
Treatment Vehicle:Gavage needle
Animal Acclimation Duration:one week
Animal Fasting:one night before experiment
Animal Endp Clinical Signs:stop breathing

Sample Preparation:

Sampleprep ID:SP001865
Sampleprep Summary:100 μL of plasma was added to tubes with ice-cold methanol (Vsample: Vextraction = 1:3). Each sample was then vortexed for 30 s, sonicated for 10 min in an ice-water bath, and incubated for 20 min at −20°C to allow protein precipitation. The mixtures were then centrifuged at 12,000 g for 10 min at 4°C.
Processing Storage Conditions:On ice
Extract Storage:On ice

Chromatography:

Chromatography ID:CH002145
Chromatography Summary:Separation was performed using a reverse-phase (C18) column (HSS T3 column; 2.1 mm × 100 mm, 1.8 μm; Waters), with a gradient elution of solution A (0.1% formic acid in water) and solution B (acetonitrile) of 0.3 mL/min and an injection volume of 5 μL. The elution gradient was set as follows: 0 min, 98% A; 1 min, 98% A; 12 min, 2% A; 16 min, 2% A; 16.1 min, 98% A; and 20 min, 98% A.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Rate:300ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Analytical Time:20min
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN002893
Analysis Type:MS
Chromatography ID:CH002145
Num Factors:8
Num Metabolites:328
Rt Units:Minutes
Units:peak area
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