Summary of Study ST001858

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001163. The data can be accessed directly via it's Project DOI: 10.21228/M8CH74 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001858
Study TitleProteasome Inhibitor Related Cardiotoxicity (part II)
Study SummaryBALB/c mice were injected with CFZ (16 mg/kg, iv), BTZ (1 mg/kg, iv), CFZ/atRA (CFZ: 16 mg/kg iv, atRA: 1 mg/kg iv) or Captisol (Vehicle, iv) (6 mice per cohort). ECGs were recorded under anesthesia at the end of 1h treatment and the mice were sacrificed by cervical dislocation. Plasma was obtained after centrifugation by heart puncture. Samples were immediately frozen and stored at -80°C until analysis
Institute
University of Zurich
Last NameMendez
First NameMax
AddressRorschacherstrasse 95. 9007, St.Gallen
Emailmaxmendez@runbox.com
Phone+41 71 494 10 67
Submit Date2021-06-27
Num Groups4
Total Subjects24
Num Females24
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-06-01
Release Version1
Max Mendez Max Mendez
https://dx.doi.org/10.21228/M8CH74
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001163
Project DOI:doi: 10.21228/M8CH74
Project Title:Proteasome Inhibitor Related Cardiotoxicity
Project Type:MS Untargeted Metabolomics from Murine Hearts
Project Summary:In this study we aimed to examine the acute metabolomic changes following proteasome inhibition with Bortezomib or Carfilzomib in murine heart.
Institute:University of Zurich
Department:Department of Hematology-Oncology. Kantonsspital St. Gallen
Laboratory:Experimental Hematolgy-KSSG
Last Name:Mendez
First Name:Max
Address:Rorschacherstrasse 95, 9007, St. Gallen.
Email:maxmendez@runbox.com
Phone:+41 71 494 10 67

Subject:

Subject ID:SU001935
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Balb/c
Age Or Age Range:8-12 weeks old
Weight Or Weight Range:20-25 g
Gender:Female
Animal Feed:ad libitum
Animal Water:ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id class
SA174247pCFZ6_POSPcaseB
SA174248pCFZ5_POSPcaseB
SA174249pCFZ2_NEGPcaseB
SA174250pCFZ3_NEGPcaseB
SA174251pCFZ6_NEGPcaseB
SA174252pCFZ4_NEGPcaseB
SA174253pCFZ4_POSPcaseB
SA174254pCFZ5_NEGPcaseB
SA174255pCFZ1_POSPcaseB
SA174256pCFZ1_NEGPcaseB
SA174257pCFZ3_POSPcaseB
SA174258pCFZ2_POSPcaseB
SA174259pCFZ.ATRA4_POSPcaseC
SA174260pCFZ.ATRA3_POSPcaseC
SA174261pCFZ.ATRA5_NEGPcaseC
SA174262pCFZ.ATRA2_POSPcaseC
SA174263pCFZ.ATRA5_POSPcaseC
SA174264pCFZ.ATRA1_POSPcaseC
SA174265pCFZ.ATRA6_NEGPcaseC
SA174266pCFZ.ATRA6_POSPcaseC
SA174267pCFZ.ATRA1_NEGPcaseC
SA174268pCFZ.ATRA3_NEGPcaseC
SA174269pCFZ.ATRA2_NEGPcaseC
SA174270pCFZ.ATRA4_NEGPcaseC
SA174271pBTZ1_NEGPcaseD
SA174272pBTZ2_NEGPcaseD
SA174273pBTZ4_NEGPcaseD
SA174274pBTZ1_POSPcaseD
SA174275pBTZ3_POSPcaseD
SA174276pBTZ2_POSPcaseD
SA174277pBTZ5_POSPcaseD
SA174278pBTZ4_POSPcaseD
SA174279pBTZ3_NEGPcaseD
SA174280pBTZ6_NEGPcaseD
SA174281pBTZ5_NEGPcaseD
SA174282pBTZ6_POSPcaseD
SA174283pCTRL5_NEGPcontrol
SA174284pCTRL1_NEGPcontrol
SA174285pCTRL3_POSPcontrol
SA174286pCTRL4_POSPcontrol
SA174287pCTRL2_POSPcontrol
SA174288pCTRL6_NEGPcontrol
SA174289pCTRL3_NEGPcontrol
SA174290pCTRL6_POSPcontrol
SA174291pCTRL4_NEGPcontrol
SA174292pCTRL5_POSPcontrol
SA174293pCTRL1_POSPcontrol
SA174294pCTRL2_NEGPcontrol
SA174295QC_15_POSQC
SA174296QC_14_POSQC
SA174297QC_13_POSQC
SA174298QC_12_POSQC
SA174299QC_06_POSQC
SA174300QC_11_NEGQC
SA174301QC_12_NEGQC
SA174302QC_10_NEGQC
SA174303QC_09_NEGQC
SA174304QC_07_NEGQC
SA174305QC_08_NEGQC
SA174306QC_13_NEGQC
SA174307QC_14_NEGQC
SA174308QC_09_POSQC
SA174309QC_10_POSQC
SA174310QC_08_POSQC
SA174311QC_07_POSQC
SA174312QC_15_NEGQC
SA174313QC_06_NEGQC
SA174314QC_11_POSQC
Showing results 1 to 68 of 68

Collection:

Collection ID:CO001928
Collection Summary:Anesthesia was induced with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5%. Plasma was obtained by centrifugation after cervical dislocation under anesthesia. Samples were snap frozen in dry ice and kept at -80°C until analysis.
Sample Type:Blood (plasma)
Storage Conditions:Described in summary

Treatment:

Treatment ID:TR001947
Treatment Summary:Injections with CFZ (16 mg/kg, iv), BTZ (1 mg/kg, iv), CFZ+atRA (CFZ: 16 mg/kg iv, atRA: 1 mg/kg iv) or Captisol (Vehicle, iv)
Treatment Compound:Bortezomib (PS-34, Selleck Chemicals, S1013); Carfilzomib (PR-171, Selleck Chemicals, S2853); all-trans-Retinoic-Acid (Sigma-Aldrich, R2625); Captisol (Ligand Pharmaceuticals
Treatment Route:i.v
Treatment Dose:As described in treatment summary
Treatment Vehicle:Captisol
Animal Anesthesia:Induction with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5%
Animal Endp Euthanasia:1h
Animal Endp Tissue Coll List:1h

Sample Preparation:

Sampleprep ID:SP001941
Sampleprep Summary:1 Take 100 μL of each sample (including QC) into the corresponding 96-well plate; 2 Add 400 μL of precipitant (methanol: acetonitrile = 2:1, V/V) to the sample, seal the membrane with a film sealer, and shake for 1 min; 3 Place the sample in ice water for ultrasonication for 10 min; 4 Place the sonicated sample at -20°C for 2h; 5 Centrifuge the sample in a centrifuge (4,000 rpm * 4°C, 30 min), each sample taken 300 μL to the new 96-well plate; 6 Put the 96-well plate into a freeze dryer and drain it; 7 On the drained plate, add 300 μL of 10% methanol, mix with a Multi-Tube vorterer (parameter setting 2,500HZ, 3 min), and place in ice water for 10 min; 8 Centrifuge for 20 min (4,000 rpm * 4°C), take 250 μL of the supernatant on a new plate; 9 For each sample (excluding preparation QC), take 50 μL of each sample and mix into a QC sample, and the prepared supernatant is ready to be used.
Processing Storage Conditions:-80℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN003011 AN003012
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Xevo-TQ Waters Xevo-TQ
Ion Mode NEGATIVE POSITIVE
Units Peak Area Peak Area

Chromatography:

Chromatography ID:CH002232
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002800
Analysis ID:AN003011
Instrument Name:Waters Xevo-TQ
Instrument Type:QTOF
MS Type:ESI
MS Comments:capillary and sampling cone voltages were set at 2.0 kV and 40.0 V. TOF mass range was from 50 to 1200 Da and the scan time was 0.2 s.
Ion Mode:NEGATIVE
  
MS ID:MS002801
Analysis ID:AN003012
Instrument Name:Waters Xevo-TQ
Instrument Type:QTOF
MS Type:ESI
MS Comments:capillary and sampling cone voltages were set at 3.0 kV and 40.0 V, respectively
Ion Mode:POSITIVE
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