Summary of Study ST001863
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001176. The data can be accessed directly via it's Project DOI: 10.21228/M8PT24 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001863 |
Study Title | Analysis of Bxa induced metabolic changes in epithelial cells |
Study Summary | Studying the metabolic impact of Bxa treatment to epithelial cells. |
Institute | Broad Institute of MIT and Harvard |
Department | Infectious Disease and Microbiome |
Laboratory | Xavier Lab |
Last Name | Brown |
First Name | Eric |
Address | 415 Main Street, Cambridge, Massachusetts, 02142, USA |
erbrown@broadinstitute.org | |
Phone | 6176826566 |
Submit Date | 2021-07-01 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-07-24 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR001176 |
Project DOI: | doi: 10.21228/M8PT24 |
Project Title: | Bxa induced change in metabolite secretion |
Project Summary: | An analysis of the secreted metabolites from HT-29 and Caco-2 cells treated with Bxa protein compared to a buffer control |
Institute: | Broad Institute of MIT and Harvard |
Department: | Infectious Disease and Microbiome |
Laboratory: | Xavier Lab |
Last Name: | Brown |
First Name: | Eric |
Address: | 415 Main Street, Cambridge, Massachusetts, 02142, USA |
Email: | erbrown@broadinstitute.org |
Phone: | 6176826566 |
Subject:
Subject ID: | SU001940 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | HT-29 and Caco-2 cells |
Species Group: | Mammals |
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA174430 | Caco2_bxa2 | Bxa |
SA174431 | Caco2_bxa1 | Bxa |
SA174432 | HT29_bxa3 | Bxa |
SA174433 | HT29_bxa1 | Bxa |
SA174434 | HT29_bxa2 | Bxa |
SA174435 | Caco2_bxa3 | Bxa |
SA174436 | Caco2_con3 | Control |
SA174437 | Caco2_con1 | Control |
SA174438 | HT29_con2 | Control |
SA174439 | HT29_con3 | Control |
SA174440 | HT29_con1 | Control |
SA174441 | Caco2_con2 | Control |
Showing results 1 to 12 of 12 |
Collection:
Collection ID: | CO001933 |
Collection Summary: | Cell supernatants were collected by centrifugation of the epithelial cells and frozen at -80 degrees until analysis. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR001952 |
Treatment Summary: | Cells were treated with 200nM Bxa protein and compared to a control of the same buffer but without protein. |
Sample Preparation:
Sampleprep ID: | SP001946 |
Sampleprep Summary: | Samples were prepped by adding isopropanol to the supernatant to extract metabolites and then injected on the machine |
Combined analysis:
Analysis ID | AN003020 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | ID-X |
Column | C8 |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Area under curve |
Chromatography:
Chromatography ID: | CH002238 |
Instrument Name: | ID-X |
Column Name: | C8 |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002809 |
Analysis ID: | AN003020 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Data were analyzed using Progenesis QI peak picking software ThermoScientific Trace Finder 5.0. |
Ion Mode: | POSITIVE |