Summary of Study ST001863
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001176. The data can be accessed directly via it's Project DOI: 10.21228/M8PT24 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001863 |
| Study Title | Analysis of Bxa induced metabolic changes in epithelial cells |
| Study Summary | Studying the metabolic impact of Bxa treatment to epithelial cells. |
| Institute | Broad Institute of MIT and Harvard |
| Department | Infectious Disease and Microbiome |
| Laboratory | Xavier Lab |
| Last Name | Brown |
| First Name | Eric |
| Address | 415 Main Street, Cambridge, Massachusetts, 02142, USA |
| erbrown@broadinstitute.org | |
| Phone | 6176826566 |
| Submit Date | 2021-07-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-07-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001176 |
| Project DOI: | doi: 10.21228/M8PT24 |
| Project Title: | Bxa induced change in metabolite secretion |
| Project Summary: | An analysis of the secreted metabolites from HT-29 and Caco-2 cells treated with Bxa protein compared to a buffer control |
| Institute: | Broad Institute of MIT and Harvard |
| Department: | Infectious Disease and Microbiome |
| Laboratory: | Xavier Lab |
| Last Name: | Brown |
| First Name: | Eric |
| Address: | 415 Main Street, Cambridge, Massachusetts, 02142, USA |
| Email: | erbrown@broadinstitute.org |
| Phone: | 6176826566 |
Subject:
| Subject ID: | SU001940 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | HT-29 and Caco-2 cells |
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA174430 | Caco2_bxa2 | Bxa |
| SA174431 | Caco2_bxa1 | Bxa |
| SA174432 | HT29_bxa3 | Bxa |
| SA174433 | HT29_bxa1 | Bxa |
| SA174434 | HT29_bxa2 | Bxa |
| SA174435 | Caco2_bxa3 | Bxa |
| SA174436 | Caco2_con3 | Control |
| SA174437 | Caco2_con1 | Control |
| SA174438 | HT29_con2 | Control |
| SA174439 | HT29_con3 | Control |
| SA174440 | HT29_con1 | Control |
| SA174441 | Caco2_con2 | Control |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO001933 |
| Collection Summary: | Cell supernatants were collected by centrifugation of the epithelial cells and frozen at -80 degrees until analysis. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001952 |
| Treatment Summary: | Cells were treated with 200nM Bxa protein and compared to a control of the same buffer but without protein. |
Sample Preparation:
| Sampleprep ID: | SP001946 |
| Sampleprep Summary: | Samples were prepped by adding isopropanol to the supernatant to extract metabolites and then injected on the machine |
Combined analysis:
| Analysis ID | AN003020 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | ID-X |
| Column | C8 |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Area under curve |
Chromatography:
| Chromatography ID: | CH002238 |
| Instrument Name: | ID-X |
| Column Name: | C8 |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002809 |
| Analysis ID: | AN003020 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were analyzed using Progenesis QI peak picking software ThermoScientific Trace Finder 5.0. |
| Ion Mode: | POSITIVE |
