Summary of Study ST001955

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001242. The data can be accessed directly via it's Project DOI: 10.21228/M85H78 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001955
Study TitleMetabonomics analysis reveals the physiological mechanism of promoting maize shoots growth under negative pressure to stabilize soil water content
Study SummaryThe purpose of this study is to analyze maize shoots growth under negative pressure to stabilize soil water content，Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system.
Institute
Heilongjiang Bayi Agricultural University
Last NameZhang
First NameJili
AddressHigh tech Zone, Longfei District, Daqing, Heilongjiang, 163319, China
Emailzhangjili12@163.com
Phone+86-13504899312
Submit Date2021-10-03
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailAPCI-MS
Release Date2021-12-15
Release Version1
Jili Zhang Jili Zhang
https://dx.doi.org/10.21228/M85H78
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001242
Project DOI:doi: 10.21228/M85H78
Project Title:Metabonomics analysis reveals the physiological mechanism of promoting maize shoots growth under negative pressure to stabilize soil water content
Project Summary:The purpose of this study is to analyze maize shoots growth under negative pressure to stabilize soil water content，Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system.
Institute:Heilongjiang Bayi Agricultural University
Last Name:Zhang
First Name:Jili
Address:High tech Zone, Longfei District, Daqing, Heilongjiang, 163319, China
Email:zhangjili12@163.com
Phone:86-13504899312

Subject:

Subject ID:SU002033
Subject Type:Plant
Subject Species:Zea mays
Taxonomy ID:4577
Species Group:Plants

Factors:

Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA184114DW3control
SA184115DW2control
SA184116DW4control
SA184117DW5control
SA184118DW6control
SA184119DW1control
SA184108SW3Moisture treatment
SA184109SW4Moisture treatment
SA184110SW6Moisture treatment
SA184111SW2Moisture treatment
SA184112SW5Moisture treatment
SA184113SW1Moisture treatment
Showing results 1 to 12 of 12

Collection:

Collection ID:CO002026
Collection Summary:A total of 121 differential metabolites (75 were increased and 46 were decreased) were identified in maize leaves cultivated under conditions of stable soil water content and dry-wet cycles.
Sample Type:new

Treatment:

Treatment ID:TR002045
Treatment Summary:Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system.

Sample Preparation:

Sampleprep ID:SP002039
Sampleprep Summary:Collected samples were thawed on ice, and metabolites were extracted from 20 µL of each sample using 120 µL of precooled 50% methanol buffer. Next, the mixture of metabolites was vortexed for 1 min, followed by incubation for 10 min at room temperature, and storing at -20°C overnight. On the next day, the mixture was centrifuged at 4,000 g for 20 min and the supernatant was subsequently transferred to 96 well plates. Samples were stored at ‐80°C prior to the LC‐MS analysis. Pooled quality control (QC) samples were also prepared by combining 10 μL of each extraction mixture

Chromatography:

Chromatography ID:CH002352
Instrument Name:TripleTOF 5600
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Chromatography Type:HILIC

Analysis:

Analysis ID:AN003180
Analysis Type:MS
Chromatography ID:CH002352
Num Factors:2
Num Metabolites:262
Units:μg/100ml
  
Analysis ID:AN003181
Analysis Type:MS
Chromatography ID:CH002352
Num Factors:2
Num Metabolites:376
Units:μg/100ml
  logo