Summary of Study ST002042

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001289. The data can be accessed directly via it's Project DOI: 10.21228/M83D80 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002042
Study TitleLong-term metabolic profiling in transient middle cerebral artery occlusion (tMCAO) mouse model
Study Summarywe investigated the long-term metabolic changes caused by stroke in a transient middle cerebral artery occlusion (tMCAO).
Institute
KIST
Last NameNaeun
First NameYoon
Address5, Hwarang ro 14 gil, songbuk-gu, Seoul
Emailbetteryun@kist.re.kr
Phone82-10-9091-5149
Submit Date2021-12-23
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-05-31
Release Version1
Yoon Naeun Yoon Naeun
https://dx.doi.org/10.21228/M83D80
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001289
Project DOI:doi: 10.21228/M83D80
Project Title:Investigation of long-term metabolic alteration after stroke in tMCAO (transient middle cerebral artery occlusion) mouse model using metabolomics approach
Project Summary:Investigation of changes in brain damage and long-term metabolic changes caused by stroke in a transient middle cerebral artery occlusion (tMCAO)
Institute:Korea institute of Science and Technology
Last Name:Yoon
First Name:Naeun
Address:5, hwa-rang ro 14 gil, Seong-buk, Seoul, 02751, Korea
Email:Southbetteryun@kist.re.kr
Phone:029585145

Subject:

Subject ID:SU002163
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id time point
SA196440N_81-day
SA196441N_71-day
SA196442sham_11-day
SA196443sham_21-day
SA196444sham_41-day
SA196445sham_31-day
SA1964461dM_11-day
SA196447N_11-day
SA196448N_21-day
SA196449N_41-day
SA196450N_51-day
SA196451N_61-day
SA196452N_31-day
SA196453sham_51-day
SA1964541dM_61-day
SA1964551dM_71-day
SA1964561dM_91-day
SA1964571dM_51-day
SA1964581dM_41-day
SA1964591dM_21-day
SA1964601dM_31-day
SA1964611dM_101-day
SA1964621dM_81-day
SA1964631dM_131-day
SA1964641dM_111-day
SA1964651dM_121-day
SA1964661mM_11-month
SA1964671mM_21-month
SA1964681mM_41-month
SA1964691mM_51-month
SA196470sham_111-month
SA1964711mM_31-month
SA196472sham_141-month
SA196473sham_131-month
SA196474sham_121-month
SA196475sham_101-month
SA1964761wM_61-week
SA1964771wM_41-week
SA1964781wM_31-week
SA1964791wM_21-week
SA1964801wM_11-week
SA1964811wM_51-week
SA196482sham_61-week
SA196483sham_81-week
SA196484sham_91-week
SA1964851wM_71-week
SA196486sham_71-week
SA1964876mM_46-month
SA1964886mM_36-month
SA1964896mM_26-month
SA1964906mM_16-month
SA1964916mM_56-month
SA1964926mM_66-month
SA1964936mS_36-month
SA1964946mS_26-month
SA1964956mS_16-month
SA1964966mS_46-month
Showing results 1 to 57 of 57

Collection:

Collection ID:CO002156
Collection Summary:Plasma was obtained from the blood samples upon centrifugation and stored at −80 °C until analysis.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR002175
Treatment Summary:The mouse model was induced via MCAO using a 6-0 nylon monolayer suture. Animals were anesthetized with 2% isoflurane in 30% oxygen and 70% nitrous oxide using a face mask. The mice were anesthetized, and blood was collected by retro-orbital bleeding using sterile capillary tubes.

Sample Preparation:

Sampleprep ID:SP002169
Sampleprep Summary:plasma samples were processed through a simple protein precipitation technique using 150 µL of ice-cold methanol using plasma methanol ratio of 1:3. After the addition of methanol, the mixture was then vortexed and centrifuged at 20,800×g for 10 min at 4 °C.

Chromatography:

Chromatography ID:CH002508
Instrument Name:Orbitrap Velos Pro mass spectrometer system
Column Name:Waters ACQUITY UPLC BEH C18
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN003393
Analysis Type:MS
Chromatography ID:CH002508
Num Factors:4
Num Metabolites:44
Units:peak area
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