Summary of Study ST002165
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001378. The data can be accessed directly via it's Project DOI: 10.21228/M8M12K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002165 |
| Study Title | Untargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours - LCMS Study. |
| Study Type | Untargeted LCMS |
| Study Summary | Untargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours. Metabolic profiling of urine samples were performed by TripleTOF 5600+ (AB Sciex) in both ionization mode |
| Institute | Institute of Nuclear Medicine and Allied Sciences |
| Department | Institute of Nuclear Medicine and Allied Sciences |
| Laboratory | Metabolomics facility |
| Last Name | Rana |
| First Name | Poonam |
| Address | DRDO, Lucknow Road, Timarpur |
| poonamrana27@gmail.com | |
| Phone | +911123905319 |
| Submit Date | 2021-11-24 |
| Num Groups | 3 |
| Total Subjects | 21 |
| Num Males | 21 |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-05-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001378 |
| Project DOI: | doi: 10.21228/M8M12K |
| Project Title: | Untargeted metabolomics following radiation exposure |
| Project Type: | Untargeted metabolic profiling |
| Project Summary: | Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours using LC-MS and NMR. |
| Institute: | Institute of Nuclear Medicine and Allied Sciences |
| Department: | Metabolomics facility |
| Laboratory: | Metabolomics facility |
| Last Name: | Rana |
| First Name: | Poonam |
| Address: | DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India |
| Email: | poonam@inmas.drdo.in |
| Phone: | +911123905319 |
| Funding Source: | INM 313, UGC-JRF |
Subject:
| Subject ID: | SU002251 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6 |
| Age Or Age Range: | 8-10 weeks |
| Gender: | Male |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA207569 | R_5 Gy 5 | 5 Gy Irradiated |
| SA207570 | R_5 Gy 6 | 5 Gy Irradiated |
| SA207571 | R_5 Gy 4 | 5 Gy Irradiated |
| SA207572 | R_5 Gy 2 | 5 Gy Irradiated |
| SA207573 | R_5 Gy 1 | 5 Gy Irradiated |
| SA207574 | R_5 Gy 7 | 5 Gy Irradiated |
| SA207575 | R_5 Gy 3 | 5 Gy Irradiated |
| SA207576 | R_7.5 Gy 1 | 7.5 Gy Irradiated |
| SA207577 | R_7.5 Gy 7 | 7.5 Gy Irradiated |
| SA207578 | R_7.5 Gy 6 | 7.5 Gy Irradiated |
| SA207579 | R_7.5 Gy 4 | 7.5 Gy Irradiated |
| SA207580 | R_7.5 Gy 2 | 7.5 Gy Irradiated |
| SA207581 | R_7.5 Gy 3 | 7.5 Gy Irradiated |
| SA207582 | R_Ctrl 7 | Control |
| SA207583 | R_Ctrl 6 | Control |
| SA207584 | R_Ctrl 5 | Control |
| SA207585 | R_Ctrl 2 | Control |
| SA207586 | R_Ctrl 3 | Control |
| SA207587 | R_Ctrl 1 | Control |
| SA207588 | R_Ctrl 4 | Control |
| SA207589 | R_ QC9 | QC |
| SA207590 | R_ QC10 | QC |
| SA207591 | R_ QC8 | QC |
| SA207592 | R_ QC7 | QC |
| SA207593 | R_ QC11 | QC |
| SA207594 | R_ QC6 | QC |
| SA207595 | R_ QC14 | QC |
| SA207596 | R_ QC16 | QC |
| SA207597 | R_ QC15 | QC |
| SA207598 | R_ QC5 | QC |
| SA207599 | R_ QC13 | QC |
| SA207600 | R_ QC12 | QC |
| Showing results 1 to 32 of 32 |
Collection:
| Collection ID: | CO002244 |
| Collection Summary: | Animals were acclimatized for 48 hrs in polypropylene cages. Urine samples were collected at 24 hrs post radiation exposure. |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
| Collection Tube Temp: | 4℃ |
Treatment:
| Treatment ID: | TR002263 |
| Treatment Summary: | Random categorization was done into three groups: control, 5 Gy (sub-lethal) and 7.5 Gy (lethal) irradiated (seven animals in each group). Mice were kept for 12 hrs light and dark cycle with room temperature (19–23°C) and humidity (45–65%) conditions properly maintained. Mice were exposed to total-body gamma radiation (field size; 35x35 cm) of 5 Gy and 7.5 Gy through Tele 60Co unit γ- irradiation facility (Bhabhatron II, Panacea Medical, Bangalore, India) with dose rate of 1 Gy/min. Control mice were sham irradiated. |
| Treatment: | Radiation exposure |
| Treatment Dose: | 5 Gy and 7.5 Gy Gamma-60Cobalt irradiation |
Sample Preparation:
| Sampleprep ID: | SP002257 |
| Sampleprep Summary: | Urine samples (50 uL) were added to 200ul of water containing internal standards (caffeine, nitrobenzoic acid and acetaminophen) and were filtered through 3 kDa filter. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | On ice |
Chromatography:
| Chromatography ID: | CH002621 |
| Instrument Name: | ExionLC |
| Column Name: | Kinetex EVO C18 (100 x 2.1 mm,1.7um) |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN003547 |
| Analysis Type: | MS |
| Acquisition Parameters File: | MS_Parameter.pdf |
| Chromatography ID: | CH002621 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST002165_AN003547_Results.txt |
| Units: | Intensity |
| Analysis ID: | AN003548 |
| Analysis Type: | MS |
| Acquisition Parameters File: | MS_Parameter.pdf |
| Chromatography ID: | CH002621 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST002165_AN003548_Results.txt |
| Units: | Intensity |
